Production and genetic improvement of a novel antimycotic agent, Saadamycin, against Dermatophytes and other clinical fungi from Endophytic Streptomyces sp. Hedaya48

Abstract

As a part of our ongoing efforts towards finding novel antimycotic agents from marine microflora of the Red Sea, vanillin, 5,7-dimethoxy-4-p-methoxylphenylcoumarin and the new antimycotic compound saadamycin were isolated from endophytic Streptomyces sp. Hedaya48. The producing strain was isolated from the Egyptian sponge Aplysina fistularis and subjected to different UV irradiation doses. A mutant strain Ah22 with 10.5-fold (420 mg/l as compared to 40 mg/l produced by the parental strain) improved saadamycin production was isolated. Production of saadamycin from mutant Ah22 was enhanced to 2.26-fold (950 mg/l) and 2.38-fold (1000 mg/l) under optimized culture conditions in batch culture and bioreactors, respectively. Both saadamycin and 5,7-dimethoxy-4-p-methoxylphenylcoumarin exhibited significant antimycotic activity against dermatophytes and other clinical fungi.

Identification and Testing of Antidermatophytic Oxaborole-6-Benzene Sulphonamide Derivative (OXBS) from Streptomyces atrovirens KM192347 Isolated from Soil

“…Aliquots of starch nitrate broth (250 mL for each) in 500 mL Erlenmeyer flasks (Gomhuria, Cairo, Egypt) were inoculated by (1% v/v) spore suspensions of actively growing cells in the mid-log phase after four days of growth of the S. atrovirens strain. The inoculated samples were incubated at different agitation rates (0, 50, 100, 150, 200, and 250 rpm) for different times (3-14 days), at different incubation temperatures (15,20,25,30,35,40,45, and 50 • C) and different pH values (4)(5)(6)(7)(8)(9)(10)(11)(12). Then CFCS were prepared by centrifuging the culture (5000Xg for 10 min at 4 • C).…”

“…Dermatophytes that have the ability to invade keratinized tissues such as skin, hair and nails of both human and animals cause dermatophytosis. The important dermatophytes genera are Trichophyton, Microsporum, and Epidermatophyton [6]. The detection of antifungal resistance within fungal pathogens is increasing [7].…”

Bioprospecting Sponge-Associated Microbes for Antimicrobial Compounds

“…Invasive candidiasis is accounted as the most common nosocomial fungal infection resulting in an average mortality rate between 25%–38% [ 103 ]. El-Gendy et al [ 107 ] isolated Streptomyces sp. Hedaya 48 from the sponge Aplysina fistularis and identified two compounds: the novel compound saadamycin ( 13 ) and the known compound 5,7-dimethoxy-4- p -methoxylphenylcoumarin ( 14 ) ( Figure 3 ).…”

“…Streptomyces sp. Hedaya48 is currently the most potent sponge-associated bacterial isolate for antifungal activities with the production of saadamycin and 5,7-dimethoxy-4- p -methoxylphenylcoumarin [ 107 ]. In addition, isolation of the anti-Trypanosoma and anti-Leishmania compounds valinomycin, staurosporine and butenolide from Streptomyces sp.…”

Marine Pharmacology in 2009–2011: Marine Compounds with Antibacterial, Antidiabetic, Antifungal, Anti-Inflammatory, Antiprotozoal, Antituberculosis, and Antiviral Activities; Affecting the Immune and Nervous Systems, and other Miscellaneous Mechanisms of Action

“…strain Hedaya 48 isolated from the Egyptian sponge Aplysina fistularis [56], and two novel depsipeptides, theopapuamides B & C ( 41 , 42 ), isolated from the Indonesian marine sponge Siliquariaspongia mirabilis [57]. Future mechanism of action studies with these potent compounds will hopefully characterize their molecular pharmacology.…”