Production and immobilization of lipase PCL and its application in synthesis of α-linolenic acid-rich diacylglycerol

Abstract

α‐Linolenic acid‐rich diacylglycerol has been demonstrated with promising health promotion functions. This study examined the production, immobilization of lipase PCL, and its application in the synthesis of diacylglycerol by esterification of α‐linolenic acid with glycerol. The resin ECR8806 was selected as an effective support for the immobilization of lipase PCL. Fourier transform infrared and Laser scanning confocal microscope analysis proved that the lipase was successfully immobilized on the resin. Compared with the free PCL, the immobilized one exhibited higher temperature tolerance. Under optimized reaction conditions, a DAG content of 54.49% were obtained. After further purified by molecular distillation, the purity of DAG was up to 99.28%. During esterification, the immobilized PCL was quite stable and retained more than 91.60% of its initial activity after 10 cycles. These new findings on the immobilized PCL will make it to be a prospective enzyme in oils and fats industry. Practical applications Immobilized PCL, a monoacylglycerol and diacylglycerol lipase, was first employed to synthesis of a high purity α‐linolenic acid‐rich diacylglycerol. The improved biocatalytic performance and reusability of the lipase make it to be a promising catalyst in industrial application.

Effect of Purification Methods on the Physicochemical and Thermodynamic Properties and Crystallization Kinetics of Medium-Chain, Medium–Long-Chain, and Long-Chain Diacylglycerols

“…The purification process can be a single step, multiple steps, or a combination of different techniques. Most of the literature reported on the effect of purification in increasing the DAG concentration or purity. ,− In some other work, characterization of purified DAG in terms of thermodynamic properties (crystallization and melting temperatures), crystal polymorphs, iodine value, fatty acid compositions, slip melting point, and solid fat content profile had been performed. , Nonetheless, a comprehensive characterization of DAG before and after purification has not been reported. It is vital to have a holistic characterization on produced DAG, especially when being applied as an emulsifier or crystallization modifier.…”

Enzymatic Synthesis of Diacylglycerol-Enriched Oil by Two-Step Vacuum-Mediated Conversion of Fatty Acid Ethyl Ester and Fatty Acid From Soy Sauce By-Product Oil as Lipid-Lowering Functional Oil

“…These results were attributable to the higher conversion of EE and FFA to DAG through a two-step vacuum-mediated catalysis by 1,3-specific lipase ANL-MARE. Previous studies have reported that a single FFA or EE are transformed into DAG through a single reaction process ( 21 , 37 ), and FFA, as an acyl donor, has shown a higher reaction rates and conversions than EE ( 42 ). However, few studies performed one-pot conversion of the mixture substrate of EE and FFA to DAG by an enzymatic method.…”

Current Status and Future Perspectives of Supports and Protocols for Enzyme Immobilization

“…Liu et al (2018) [244] immobilized lipases on electrospray fibers with some reinforcement materials such as P(GMA-co-MA)-g-PEO (poly(glycidyl methacrylate-co-methyl acrylate)-g-polyethylene oxide). It is noteworthy that the activity and stability of the derivative were improved, and consequently, the derivative became more versatile, able to be applied in various fields.…”

“…Biopolymers and biocomposites are used to produce different types of lipase matrices, conjugated or not, with a broad range of ligands of distinguished properties and nature, as mentioned in recent studies: membranes, resins, nanofibers, beads, nanoparticles, nanotubes, scaffolds, colloidal particles, and nanowhiskers [203,244,298,305,308,309,312]. In the study of Elias et al (2018) [309], the biocatalyst consisting of a biopolymer carrier of nanocellulose/chitosan was prepared by extraction of nanocellulose from palm oil frond leaves, followed by consecutive crosslinking with chitosan and the Candida rugosa lipase, both using glutaraldehyde as crosslinking agent [309,320].…”