Production and Performance of Gynogenetic Sugarbeet Lines

Abstract

In vitro techniques were used to obtain haploid and doubled haploid lines of gynogenetic origin in sugarbeet (Beta vulgaris L.). Two pathways of regeneration were available for unfertilized ovule culture-embryogenesis and organogenesis. Regenerants were obtained from pri mary explants of diploid and tetraploid donor plants on MS nutrient medium supplemented with benzyla minopurine and sucrose. Flow cytometry indicated spontaneous polyploidization in all primarily haploid ovule-derived lines that originated from Belarusian culti vars. Mixoploidy that occured in doubled haploids was overcome through micropropagating apices of generative shoots. Performance of sugarbeet plants of ovule origin and hybrids produced with these lines indicated that prom ising breeding lines could be selected from gynogenetic lines.

Registration of Sugarbeet Doubled Haploid Line KDH13 with Resistance to Beet Curly Top

“…hree of the 20 plantlets were diploid. Diplodization was due to spontaneous chromosomal doubling, an established phenomenon in tissue culture (Svirshchevskaya and Dolezel, 2000). he remaining 17 haploid plants were treated with colchicine (Hansen et al, 1995) for diploidization but without success.…”

Registration of Sugarbeet Doubled Haploid Line KDH13 with Resistance to Beet Curly Top

“…Three of the 20 plantlets were diploid. Diplodization was due to spontaneous chromosomal doubling, an established phenomenon in tissue culture (Svirshchevskaya and Dolezel, 2000). The remaining 17 haploid plants were treated with colchicine (Hansen et al, 1995) for diploidization but without success.…”

An EMS mutagenesis protocol for sugar beet and isolation of non-bolting mutants

“…Here, seeds were chosen because microspore or anther culture of beet is not possible. Doubled haploids can only be produced by cultivating unpollinated ovules (Lux et al 1990, Svirshchevskaya andDolezel 2000).…”