Production and properties of inulinase fromPenicillium sp. NFCC 2768 grown on inulin-rich vegetal infusions

Continuous generation of fructose from Taraxacum officinale tap root extract and inulin by immobilized inulinase in a packed-bed reactor

“…dandelion, dahlia, asparagus, sunflower, lettuce root and Jerusalem artichoke (Housseiny, 2014;Mansouri et al, 2013;Rawat et al, 2015b;Singh & Singh, 2014). Kango (2008) reported A. niger NK-126, an isolate from onion peels, to produce 55 U/ml of inulinase in four days using dandelion tap root extract.…”

“…Protein precipitate thus obtained was suspended in appropriate volume of sodium acetate buffer (200 mM, pH 5.0). Inulinase was partially purified by precipitating total protein using ethanol followed by fractionation using DEAECellulose-52 (Hi-media, Mumbai) ion exchange chromatographic column of size 2.5×50 cm (internal diameter × height) (Rawat et al, 2015b). Optical densities of eluted column fractions were measured at 280 nm and these were examined for protein concentration (Lowry, 1951) and inulinase activity.…”

Optimization of Inulin Hydrolysis by Penicillium lanosocoeruleum Inulinases and Efficient Conversion Into Polyhydroxyalkanoates

“…A novel strain of Penicillium subrubescens (FBCC 1632 T ) isolated from soil has been found to produce up to 7.7 U/mL −1 when tested on pure inulin ( Mansouri et al, 2013 ), while a production level of 1 U/mL −1 has been reported for Penicillium sp. NFCC 2768, and this strain is much more effective as inulinase producer (up to 3.9 U/mL −1 ) when grown on inulin-rich vegetable infusions with respect to pure inulin ( Rawat et al, 2015b ). Similarly, four Penicillium strains, selected from a fungal library of inulinase producers on inulin-rich plant extract, displayed a production level ranging from 0.5 to 2.7 U/mL −1 ( Rawat et al, 2015a ).…”

“…A similar profile was already reported for inulinase production in shake-flask fermentations of many fungal species, i.e., Penicillium sp. ( Rawat et al, 2015b ), Aspergillus fumigatus ( Chikkerur et al, 2018 ), Aspergillus niger ( Mahmoud et al, 2011 ), and Aspergillus tritici ( Singh et al, 2020b ).…”

Response surface methodology to optimize inulinase production by a newly isolated Penicillium amphipolaria strain by solid-state fermentation of Saccharum arundinaceum

“…An I/S ratio of 2.56 was obtained from A. avus ATCC 16883 [13], a ratio of 3.38 was obtained from P. oxalicum BGPUP-4 [21], a ratio in the range of 0.53-0.66 was obtained from Penicillium sp. NFCC 2768 [34], a ratio of 0.57-2.49 was obtained from Rhizopus microspores 13aIV [35].…”