Production and Purification of Cellulase Enzyme by Endophytic<i>Bacillus</i>sp. Isolated from<i>Rhizophora Mucronata</i>

Anu, C. J.; Christy, Priscilla Helen; Jijo, C.J.

doi:10.5958/2230-732x.2014.00257.5

Cited by 3 publications

(1 citation statement)

References 6 publications

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“…In a similar study, the specific activity of the cellulase enzyme from Bacillus sp. was 41.25 IU/mg after dialysis [ 48 ]. In the current study, the Sephadex G-100 was used to concentrate the purified cellulase enzyme, which was collected on 10 fractions, and the highest cellulase activity was 92.02 U/mL with a specific activity of 110.95 U/mg, as shown in Table 5 .…”

Section: Resultsmentioning

confidence: 99%

Synthesis, Optimization, and Characterization of Cellulase Enzyme Obtained from Thermotolerant Bacillus subtilis F3: An Insight into Cotton Fabric Polishing Activity

Fouda,

Alshallash,

Atta

et al. 2023

J. Microbiol. Biotechnol.

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The efficacy of 40 bacterial isolates obtained from hot spring water samples to produce cellulase enzymes was investigated. As a result, the strain Bacillus subtilis F3, which was identified using traditional and molecular methods, was selected as the most potent for cellulase production. Optimization was carried out using one-factor-at-a-time (OFAT) and BOX-Behnken Design to detect the best conditions for the highest cellulase activity. This was accomplished after an incubation period of 24 h at 45°C and pH 8, with an inoculum size of 1% (v/v), 5 g/l of peptone as nitrogen source, and 7.5 g/l of CMC. Moreover, the best concentration of ammonium sulfate for cellulase enzyme precipitation was 60% followed by purification using a dialysis bag and Sephadex G-100 column chromatography to collect the purified enzyme. The purified cellulase enzyme was characterized by 5.39-fold enrichment, with a specific activity of 54.20 U/mg and a molecular weight of 439 kDa. There were 15 amino acids involved in the purified cellulase, with high concentrations of 160 and 100 mg/l for glycine and proline respectively. The highest stability and activity of the purified cellulase was attained at pH 7 and 50°C in the presence of 150 ppm of CaCl 2 , NaCl, and ZnO metal ions. Finally, the biopolishing activity of the cellulase enzyme, as indicated by weight loss percentages of the cotton fabric, was dependent on concentration and treatment time. Overall, the thermotolerant B. subtilis F3 strain has the potential to provide highly stable and highly active cellulase enzyme for use in biopolishing of cotton fabrics.

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Section: Resultsmentioning

confidence: 99%

Synthesis, Optimization, and Characterization of Cellulase Enzyme Obtained from Thermotolerant Bacillus subtilis F3: An Insight into Cotton Fabric Polishing Activity

Fouda,

Alshallash,

Atta

et al. 2023

J. Microbiol. Biotechnol.

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Diversity and Biotechnological Potential of Endophytic Microorganisms Associated with Tropical Mangrove Forests

Sebastianes

Azevedo

Lacava

2017

Diversity and Benefits of Microorganisms From the Tropics

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Microbial cellulases – Diversity & biotechnology with reference to mangrove environment: A review

Behera

Sethi²,

Mishra³

et al. 2017

Journal of Genetic Engineering and Biotechnology

220

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Cellulose is an abundant natural biopolymer on earth, found as a major constituent of plant cell wall in lignocellulosic form. Unlike other compounds cellulose is not easily soluble in water hence enzymatic conversion of cellulose has become a key technology for biodegradation of lignocellulosic materials. Microorganisms such as aerobic bacteria, fungi, yeast and actinomycetes produce cellulase that degrade cellulose by hydrolysing the β-1, 4-glycosidic linkages of cellulose. In contrast to aerobic bacteria, anaerobic bacteria lack the ability to effectively penetrate into the cellulosic material which leads to the development of complexed cellulase systems called cellulosome. Among the different environments, the sediments of mangrove forests are suitable for exploring cellulose degrading microorganisms because of continuous input of cellulosic carbon in the form of litter which then acts as a substrate for decomposition by microbe. Understanding the importance of cellulase, the present article overviews the diversity of cellulolytic microbes from different mangrove environments around the world. The molecular mechanism related to cellulase gene regulation, expression and various biotechnological application of cellulase is also discussed.

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Production and Purification of Cellulase Enzyme by EndophyticBacillussp. Isolated fromRhizophora Mucronata

Cited by 3 publications

References 6 publications

Synthesis, Optimization, and Characterization of Cellulase Enzyme Obtained from Thermotolerant Bacillus subtilis F3: An Insight into Cotton Fabric Polishing Activity

Synthesis, Optimization, and Characterization of Cellulase Enzyme Obtained from Thermotolerant Bacillus subtilis F3: An Insight into Cotton Fabric Polishing Activity

Diversity and Biotechnological Potential of Endophytic Microorganisms Associated with Tropical Mangrove Forests

Microbial cellulases – Diversity & biotechnology with reference to mangrove environment: A review

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