Production and Purification of Novel Hypocholesterolemic Peptides from Lactic Fermented Spirulina platensis through High Hydrostatic Pressure-Assisted Protease Hydrolysis

Chen, Guan-Wen; Yang, Meng-Hsuan

doi:10.3390/catal11080873

Cited by 12 publications

(4 citation statements)

References 46 publications

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“…Released α-amino group content, which represents the reactive amino acid, peptide, or protein containing a primary amine was determined with slight modifications through the methods used by Church et al 8 and Chen & Yang. 9 The reaction was conducted using o-phthaldialdehyde reagent added to the sample. The OPA adducted content was quantified by measuring the absorbance value at 340 nm and using Leu-Gly as a standard.…”

Section: Determination Of Soluble Protein α-Amino Group and Amino Aci...mentioning

confidence: 99%

The protein profile of perch essence and its improvement of metabolic syndrome in vitro

Lin¹,

Chang²,

Chang³

et al. 2022

MOJFPT

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The objective of this study was to analyze the protein profile and amino acid composition of perch essence processed by high-pressure extraction compared with mackerel, and milkfish. The inhibitory activities of three essences were determined against digestive enzymes relevant to the metabolic syndrome such as the angiotensin-converting enzyme (ACE), pancreatic lipase, α-amylase, and α-glucosidase. The results showed that perch essence exhibits the highest recovery by using the same extraction method for all three fish species. The amounts of all hydrolyzed amino acids, except histidine, were approximately double in perch compared with the other two fish. The perch essence contained the most abundant soluble protein and amino acids composition related to metabolic syndrome. In vitro anti-hypertensive, hypoglycemic, and anti-obesity potency of perch essence exhibited the highest among the three fishes. Although the amounts of free amino acids were similar in the three fish species, the molecular weight distribution from gel filtration chromatographic analysis indicated that perch essence contained 32.4% of peptides with a molecular weight of less than 2.3 kDa. This study suggested that naturally occurring bioactive peptides in perch essence might potentially serve as a good source of functional food supplements for metabolic syndrome.

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Section: Determination Of Soluble Protein α-Amino Group and Amino Aci...mentioning

confidence: 99%

The protein profile of perch essence and its improvement of metabolic syndrome in vitro

Lin¹,

Chang²,

Chang³

et al. 2022

MOJFPT

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“…In vitro investigations indicated that HPP and SGQR suppressed the HMGCoAR activity, declined HMGCoAR and squalene synthases’ messenger RNA (mRNA) and protein expression (triple drop), and promoted the expression of low‐density lipoprotein receptor (LDLR) (1.2‐ to 1.8‐fold). The research led by Chen and Yang (2021) focused on the proteolytic potential of lactic fermented Spirulina platensis hypocholesterolemic peptides by the high hydrostatic pressure‐assisted (HHP; 100 or 0.1 MPa) protease (Peptidase R) hydrolysis for 6 h (HHP‐FH‐PR6) hydrolysis, via HMGCoAR inhibitory action. The capability of HMGCoAR inhibition was favorable with the IC 50 value of 2.9 μg/ml.…”

Section: Health‐promoting Effects Of Food Bapsmentioning

confidence: 99%

An updated review on food‐derived bioactive peptides: Focus on the regulatory requirements, safety, and bioavailability

Patil

Usman

Zhang

et al. 2022

Comp Rev Food Sci Food Safe

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Food‐derived bioactive peptides (BAPs) are recently utilized as functional food raw materials owing to their potential health benefits. Although there is a huge amount of scientific research about BAPs’ identification, purification, characterization, and physiological functions, and subsequently, many BAPs have been marketed, there is a paucity of review on the regulatory requirements, bioavailability, and safety of BAPs. Thus, this review focuses on the toxic peptides that could arise from their primary proteins throughout protein extraction, protein pretreatment, and BAPs’ formulation. Also, the influences of BAPs’ length and administration dosage on safety are summarized. Lastly, the challenges and possibilities in BAPs’ bioavailability and regulatory requirements in different countries were also presented. Results revealed that the human studies of BAPs are essential for approvals as healthy food and to prevent the consumers from misinformation and false promises. The BAPs that escape the gastrointestinal tract epithelium and move to the stomach are considered good peptides and get circulated into the blood using different pathways. In addition, the hydrophobicity, net charge, molecular size, length, amino acids composition/sequences, and structural characteristics of BAPs are critical for bioavailability, and appropriate food‐grade carriers can enhance it. The abovementioned features are also vital to optimize the solubility, water holding capacity, emulsifying ability, and foaming property of BAPs in food products. In the case of safety, the possible allergenic and toxic peptides often exhibit physiological functions and could be produced during the hydrolysis of food proteins. It was also noted that the production of iso‐peptides bonds and undesirable Maillard reaction might occur during protein extraction, sample pretreatments, and peptide synthesis.

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“…An ELISA was performed to evaluate the allergenic property of the treated b-LG using the method of Chen and Yang [17]. To estimate the amount of b-LG hydrolysates-IgE in the samples, each polystyrene microplate well was precoated by 50 µL of b-LG-specific IgE in phosphate buffer (20 mM, pH 7.5) and then washed three times using the buffer including 0.05% (w/v) Tween 20.…”

Section: Estimation Of Peptide Molecular Weight (Mw) Distributionmentioning

confidence: 99%

“…For example, the anti-inflammatory effect and antioxidant activity of phosvitin have been improved by HHP [16]. However, to date, it is still unclear how hydrolysis under HHP contributes to more effective proteolysis [17].…”

Section: Introductionmentioning

confidence: 99%

Proteolysis of β-Lactoglobulin Assisted by High Hydrostatic Pressure Treatment for Development of Polysaccharides-Peptides Based Coatings and Films

Yang

Niazi

et al. 2022

Coatings

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Peptides usually have many bioactive functions. The variety of peptide binding and the modularity of the components allow for their application to additional tissues and materials; hence broadening the range of possible coatings and films. β-lactoglobulin (b-LG) forms spherical microgels or can be used in the formation of coated particles, with the core formed by aggregated b-LG and the coat by polysaccharides. The enzymatic proteolysis of b-LG assisted by high hydrostatic pressure (HHP) treatment was studied. Pretreatment of HHP enhanced the hydrolysis degree (DH) of b-LG. The highest value of DH without pretreatment was 24.81% at 400 MPa, which increased to 27.53% at 200 MPa with pretreatment, suggesting a difference in the DH of b-LG caused by the processing strategy of HHP. Molecular simulation suggested that the flexible regions of b-LG, e.g., Leu140-Ala142 and Asp33-Arg40, might contribute to enzymatic proteolysis. The b-LG hydrolysate exhibited the highest capacity of scavenging free DPPH and OH radicals at 200 MPa. In addition, the 1–2 kDa and 500–1000 Da peptides fractions significantly increased from 10.53% and 9.78% (under 0.1 MPa) to 12.37% and 14.95% under 200 MPa, respectively. The higher yield of short peptides under HHP contributed to the antioxidant capacity of b-LG hydrolysates. Enzymatic hydrolysis also largely reduced the immunoreactivity of b-LG, which is of high importance in the practical application of b-LG in the field of coatings and films in regard to biocompatibility. Hydrolysis of b-LG assisted by high-pressure treatment showed promising potential in the preparation of bioactive peptides for further development of polysaccharide-peptide-based coatings and films.

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Production and Purification of Novel Hypocholesterolemic Peptides from Lactic Fermented Spirulina platensis through High Hydrostatic Pressure-Assisted Protease Hydrolysis

Cited by 12 publications

References 46 publications

The protein profile of perch essence and its improvement of metabolic syndrome in vitro

The protein profile of perch essence and its improvement of metabolic syndrome in vitro

An updated review on food‐derived bioactive peptides: Focus on the regulatory requirements, safety, and bioavailability

Proteolysis of β-Lactoglobulin Assisted by High Hydrostatic Pressure Treatment for Development of Polysaccharides-Peptides Based Coatings and Films

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