Production and Purification of Statins from Pleurotus ostreatus (Basidiomycetes) Strains

Alarcón, Julio; Águila, Sergio; Arancibia-Ávila, Patricia; Fuentes, Oscar; Zamorano-Ponce, Enrique; Hernández, Margarita

doi:10.1515/znc-2003-1-211

Cited by 72 publications

(44 citation statements)

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“…43 A synthetic compound, modeled from the structure of the natural statins, is Lipitor, the leading drug of the entire pharmaceutical industry in terms of market (∼ $14 billion per year) for many years.…”

Section: Anti-rejection Drugs (Agents That Suppress the Immune System)mentioning

confidence: 99%

Production of valuable compounds by molds and yeasts

Demain

Martens

2016

J Antibiot

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where he has contributed in a major way to the reputation of this department for many years. He also served as an Adjunct Professor at the University of Geneva. Among Julian's areas of study and accomplishment are fungal toxins including α-sarcin, chemical synthesis of triterpenes, mode of action of streptomycin and other aminoglycoside antibiotics, biochemical mechanisms of antibiotic resistance in clinical isolates of bacteria harboring resistance plasmids, their origins and evolution, secondary metabolism of microorganisms, structure and function of bacterial ribosomes, antibiotic resistance mutations in yeast ribosomes, cloning of resistance genes from an antibiotic-producing microbe, gene cloning for industrial purposes, engineering of herbicide resistance in useful crops, bleomycin-resistance gene in clinical isolates of Staphylococcus aureus and many other topics. He has been an excellent teacher, lecturing in both English and French around the world, and has organized international courses. Julian has also served on the NIH study sections, as Editor for several international journals, and was one of the founders of the journal Plasmid. We expect the impact of Julian's accomplishments to continue into the future.

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Section: Anti-rejection Drugs (Agents That Suppress the Immune System)mentioning

confidence: 99%

Production of valuable compounds by molds and yeasts

Demain

Martens

2016

J Antibiot

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“…Los cuerpos fructíferos (1.5 kg) fueron macerados en una solución de HCl (2 L, 0.1 N, pH 2-3) durante 24 h en agitación (Alarcón et al, 2003). La extracción de compuestos se hizo con acetato de etilo (1 L) hasta agotamiento.…”

Section: Extracción De Compuestosunclassified

“…Fruiting bodies (1.5 kg) were macerated in an HCI solution (2 L, 0.1 N, pH 2-3) for 24 h with stirring (Alarcón et al, 2003). Compound extraction was done with ethyl acetate (1 L) until exhaustion.…”

Section: Extraction Of Compoundsmentioning

confidence: 99%

“…Por otro lado, durante la década pasada, P. ostreatus fue catalogado como alimento funcional, al igual que otros hongos, dados sus efectos benéficos para la salud. La propiedad principal que se atribuye a la especie es la capacidad de reducir el colesterol debido a la presencia de lovastatina (Alarcón et al, 2003;Trigos & Suárez, 2010;Wasser, 2011). Asimismo, los extractos de dicho hongo han sido investigados por sus propiedades hipoglucemiante, antitrombótica, antiinflamatoria, antioxidante e inmunomoduladora, lo que le confiere potencial terapéutico para el tratamiento de varias enfermedades crónico-degenerativas (Chegwin-Angarita, Nieto-Ramirez, Atehortúa, & Sepúlve-da, 2012).…”

Section: Introductionunclassified

“…On the other hand, P. ostreatus was classified in the past decade, like other mushrooms, as a functional food due to its beneficial health effects. The main property attributed to this species is the ability to lower cholesterol due to the presence of lovastatin (Alarcón et al, 2003;Trigos & Suárez, 2010;Wasser, 2011). In addition, extracts of this mushroom have been investigated for their hypoglycemic, antithrombotic, anti-inflammatory, antioxidant and immunomodulatory properties, which give it therapeutic potential for the treatment of several chronic degenerative diseases (Chegwin-Angarita, Nieto-Ramirez, Atehortúa, & Sepúlveda, 2012).…”

Section: Introductionmentioning

confidence: 99%

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AISLAMIENTO E IDENTIFICACIÓN DE ESTEROLES DE UNA CEPA COMERCIAL DE Pleurotus sp.

Márquez-Fernández

Pacheco

Trigos

2014

Rev Cha Se Cie For y del Amb

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RESUMENC inco compuestos de una cepa comercial del hongo Pleurotus sp. se obtuvieron a partir de extracciones con acetato de etilo y metanol. Los compuestos se aislaron por medio de técnicas como la cromatografía en columna y en capa fina. Los siguientes esteroles fueron identificados mediante espectroscopía RMN 1 H: 1) ergosta-5, 7, 22-trien-3β-ol (ergosterol), 2) 5α, 8α-epidioxi-22E-ergosta-6, 22-dien-3β-ol (peróxido de ergosterol), 3) 3β, 5α, 6β, 9α-tetrahidroxiergosta-7, 22-dieno, 4) 3β, 5α, 6β, 9α-tetrahydroxyergosta-7, 22-dien y 5) 3β, 5α, 9α-trihidroxiergosta-7, 22-dien-6-ona. ABSTRACTF ive compounds in a commercial strain of the mushroom Pleurotus sp. were obtained from ethyl acetate and methanol extracts. The compounds were isolated using techniques such as column and thin layer chromatography. The following sterols were identified by 1 H NMR spectroscopy: 1) ergosta-5,7,22-trien-3β-ol (ergosterol), 2) 5α, 8α-epidioxy-22E-ergosta-6, 22-dien-3β-ol (ergosterol peroxide), 3) 3β,5α,6β,9α-tetrahidroxiergosta-7,22-dieno, 4) 3β, 5α, 6β, 9α-tetrahydroxyergosta-7, 22-dien and 5) 3β, 5α, 9α-trihidroxiergosta-7,22-dien-6-one.

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Study on the 3‐hydroxy‐3‐methyl‐glutaryl CoA reductase inhibitory properties of Agaricus bisporus and extraction of bioactive fractions using pressurised solvent technologies

et al. 2013

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BACKGROUND Agaricus bisporus mushrooms were able to lower cholesterol levels in hypercholesterolaemic rats and it was suggested that dietary fibre might inhibit cholesterol absorption. However, A. bisporus extracts were also able to inhibit the 3‐hydroxy‐3‐methyl‐glutaryl CoA reductase (HMGCR, the key enzyme in the cholesterol biosynthetic pathway) and this might also contribute to the observed lowering of cholesterol levels in serum. RESULTS The methanol–water extracts obtained from A. bisporus were able to inhibit up to 60% the HMGCR activity using an in vitro assay. The HMGCR inhibitory capacities depended on cultivation conditions, strains, etc. The potential inhibitors were not statins, they might be β‐glucans able to scavenge the substrate and impair the enzymatic reaction. They were present during all mushroom developmental stages and similarly distributed through all the tissues including the parts discarded as a by‐product. Accelerated solvent extractions using 1:1 ethanol–water as pressurised solvent (10.7 MPa, 25°C, five cycles of 5 min) were more effective in the extraction of the HMGCiR inhibitor(s) than supercritical fluid extractions (9 MPa, 40°C) using CO2 with 10% ethanol. CONCLUSION A mushroom cultivation and two extraction procedures were optimised to obtain fractions from A. bisporus with high HMGCR inhibitory activities to design novel ingredients for hypocholesterolaemic functional foodstuffs. © 2013 Society of Chemical Industry

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Production and Purification of Statins from Pleurotus ostreatus (Basidiomycetes) Strains

Abstract: Pleurotus ostreatus strains were cultured in liquid medium and on wheat straw. The yields of lovastatin were compared.

Cited by 72 publications

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Production of valuable compounds by molds and yeasts

Production of valuable compounds by molds and yeasts

AISLAMIENTO E IDENTIFICACIÓN DE ESTEROLES DE UNA CEPA COMERCIAL DE Pleurotus sp.

Study on the 3‐hydroxy‐3‐methyl‐glutaryl CoA reductase inhibitory properties of Agaricus bisporus and extraction of bioactive fractions using pressurised solvent technologies

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