Study on the 3‐hydroxy‐3‐methyl‐glutaryl <scp>CoA</scp> reductase inhibitory properties of <i>Agaricus bisporus</i> and extraction of bioactive fractions using pressurised solvent technologies

Gil-Ramírez, Alicia; Clavijo, Cristina; Marimuthu, Ponnusamy; Ruiz-Rodrı́guez, Alejandro; Navarro-Rubio, María; Pérez, María Paz Sáez; Marín, Francisco; Reglero, Guillermo; Soler‐Rivas, Cristina

doi:10.1002/jsfa.6102

Cited by 26 publications

(22 citation statements)

References 31 publications

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“…This indication was also noticed in previous studies . The ExB extract was also able to inhibit 59% of the enzyme activity indicating that perhaps other compounds soluble in supercritical CO 2 (more lipophilic molecules) were also interfering with the HMGCR activity as was also previously noticed by Gil‐Ramirez et al (2013) for SFE extracts obtained from Agaricus bisporus .…”

Section: Resultssupporting

confidence: 85%

Extraction of bioactive compounds against cardiovascular diseases from Lentinula edodes using a sequential extraction method

Morales

Piris

Ruiz-Rodrı́guez

et al. 2018

Biotechnology Progress

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Three extraction methods were sequentially combined to obtain fractions from Lentinula edodes (shiitake mushrooms) containing bioactive compounds against cardiovascular diseases (CVDs). Fruiting bodies were first extracted with plain water, obtained residue was then submitted to supercritical fluid extraction (SFE) and remaining residue submitted to hot water extraction. Sequential design allowed reutilization of the nonextracted material as raw material for the successive extractions increasing extraction yields and separating interesting compounds. Obtained fractions contained different amounts of ß-glucans, chitins, eritadenine, lenthionine, ergosterol, proteins/peptides and phenolic compounds conferring them different bioactivities. Water soluble fractions showed high antioxidant activities (ABTS and DPPH scavenging capacity and reducing power), they were also able to inhibit one of the main enzymes involved in hypertension (angiotensin-I converting enzyme) and the key enzyme of cholesterol metabolism (3-hydroxy-3-methylglutaryl coenzyme A reductase). The latter inhibitory activity was also noticed in SFE extracts although ergosterol and other lipid-like molecules were isolated. Dietary fibers were separated in the third extraction. Therefore, with this sequential extraction procedure bioactive compounds against CVDs can be selectively separated from a single batch of shiitake powder. © 2018 American Institute of Chemical Engineers Biotechnol. Prog., 34:746-755, 2018.

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Section: Resultssupporting

confidence: 85%

Extraction of bioactive compounds against cardiovascular diseases from Lentinula edodes using a sequential extraction method

Morales

Piris

Ruiz-Rodrı́guez

et al. 2018

Biotechnology Progress

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“…In addition, polyunsaturated fatty acids are present in sufficient quantity to bind to the bad cholesterol present in the human body and to remove it successfully from the organism [32]. Different studies describe this beneficial effect of fungi on blood cholesterol levels [33][34]. Among the different species of fungi, the Pleurotus species is distinguished as a potential regulator of cholesterol metabolism.…”

Section: Discussionmentioning

confidence: 99%

Study of Acute Toxicity and the Effect of the Aqueous Extract of a Formulation of Three Edibles Mushrooms on Oxidative Stress Induced in Rats

Blanche¹

2019

WJFST

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Edible mushrooms, especially Pleurotus spp. contain a wide variety of biomolecules that can play a protective role against oxidative damage. We investigate the effect of formulation of the aqueous extract of three edibles mushrooms commonly consumed in Cameroon on oxidative stress induced in rats induced by lead acetate: Pleurotus pulmonarius, Pleurotus floridanus and Pleurotus sajor-caju. The formulation made of the three species (1w:1w:1w) were dissolved in distilled water for acute toxicity (at 2000 mg/kg of bw) and protective effect (at 400 mg/kg of bw for 21 days) against oxidative stress induced in vivo with lead acetate (35 mg/kg of bw for 3 days). Several parameters were investigated in the serum and organs homogenous such as lipid profile, hepatic and oxidative stress markers. No particular sign of toxicity of the extract were revealed. After lead administration, significant increases were found in serum transaminases activity, creatinine, and lipid profile associated with a decrease of serum protein content and High Density Lipoprotein. In addition, significant increase in lipid peroxidation expressed as the concentration of malondialdehyde (MDA) and decreased level of reduced glutathione (GSH), superoxide dismutase (SOD) and catalase (CAT) activities in serum, liver and kidney showed oxidative stress due to lead exposure. The pre-treatment with aqueous extract of the mushroom's formulation showed protective effects against lead induced hepato-renal damage by improving antioxidant parameters, as well as ascorbic acid used as a reference. In conclusion, these mushrooms may be used as a natural source of antioxidants for food supplements against oxidative stress.

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“…However, ergosterol was present in the hexane fraction, which might be regarded as an inhibitor of HMGR activity. [40][41][42]…”

Section: A In Vitro Studiesmentioning

confidence: 99%

Evaluation of the Antioxidative and Hypo-cholesterolemic Effects of Lingzhi or Reishi Medicinal Mushroom, Ganoderma lucidum (Agaricomycetes), in Ameliorating Cardiovascular Disease

Rahman

Abdullah

Aminudin

2018

Int J Med Mushrooms

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Oxidative stress (OS) and hypercholesterolemia have been linked with a heightened risk of cardiovascular disease (CVD). Because of the numerous drawbacks of synthetic antioxidants and cholesterol-lowering drugs, natural antioxidative and hypocholesterolemic agents are of immense importance. This study was designed to determine both the OS-attenuating and cholesterol-lowering capacities of the hot water extract (HWE) and of five solvent-solventpartitioned fractions of Ganoderma lucidum. In vitro antioxidative performance of G. lucidum HWE and fractions was measured through DPPH free radical scavenging, Folin-Ciocalteu assay, lipid peroxidation inhibition, and human low-density lipoprotein (LDL) oxidation inhibition. In vivo antioxidative performance of G. lucidum was assessed by measuring the plasma and liver antioxidative enzymatic activities (catalase, glutathione peroxidase, and superoxide dismutase) in G. lucidum HWE-fed rats. In the CVD tests, the HWE at 200 mg/kg b.w. lowered plasma levels of total cholesterol, triacylglycerol, and LDL cholesterol and increased high-density lipoprotein cholesterol. The current findings indicate the therapeutic potentiality of G. lucidum as an OS-attenuating and hypocholesterolemic agent en route to withstanding CVD complications.

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Study on the 3‐hydroxy‐3‐methyl‐glutaryl CoA reductase inhibitory properties of Agaricus bisporus and extraction of bioactive fractions using pressurised solvent technologies

Cited by 26 publications

References 31 publications

Extraction of bioactive compounds against cardiovascular diseases from Lentinula edodes using a sequential extraction method

Extraction of bioactive compounds against cardiovascular diseases from Lentinula edodes using a sequential extraction method

Study of Acute Toxicity and the Effect of the Aqueous Extract of a Formulation of Three Edibles Mushrooms on Oxidative Stress Induced in Rats

Evaluation of the Antioxidative and Hypo-cholesterolemic Effects of Lingzhi or Reishi Medicinal Mushroom, Ganoderma lucidum (Agaricomycetes), in Ameliorating Cardiovascular Disease

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