Production and recovery of poly‐3‐hydroxybutyrate from <i>Methylobacterium</i> sp V49

Ghatnekar, M S; Pai, J. S.; Ganesh, Mani

doi:10.1002/jctb.570

Cited by 63 publications

(19 citation statements)

References 14 publications

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“…The methods of cell disruption can be divided into chemical (by using hypochlorite, acids, alkalis, or surfactants),74–76 enzymatic,77 and mechanical processes 78. All of these methods were developed with the purpose to dissolve or destroy the nonpolymeric cell materials (NPCMs).…”

Section: Polymer Recovery and Characterizationmentioning

confidence: 99%

Recent Advances in Polyhydroxyalkanoate Production by Mixed Aerobic Cultures: From the Substrate to the Final Product

Dias

Lemos

Serafim

et al. 2006

Macromolecular Bioscience

262

139

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Numerous bacteria have been found to exhibit the capacity for intracellular polyhydroxyalkanoates (PHA) accumulation. Current methods for PHA production at the industrial scale are based on their synthesis from microbial isolates in either their wild form or by recombinant strains. High production costs are associated with these methods; thus, attempts have been made to develop more cost-effective processes. Reducing the cost of the carbon substrates (e.g., through feeding renewable wastes) and increasing the efficiency of production technologies (including both fermentation and downstream extraction and recovery) are two such examples of these attempts. PHA production processes based on mixed microbial cultures are being investigated as a possible technology to decrease production costs, since no sterilization is required and bacteria can adapt quite well to the complex substrates that may be present in waste material. PHA accumulation by mixed cultures has been found under various operational conditions and configurations at both bench-scale and full-scale production. The process known as "feast and famine" or as "aerobic dynamic feeding" seems to have a high potential for PHA production by mixed cultures. Enriched cultures submitted to a transient carbon supply can synthesize PHA at levels comparable to those of pure cultures. Indeed, the intracellular PHA content can reach around 70% of the cell dry weight, suggesting that this process could be competitive with pure culture PHA production when fully developed. Basic and applied research of the PHA production process by mixed cultures has been carried out in the past decade, focusing on areas such as microbial characterization, process configuration, reactor operational strategies, process modeling and control, and polymer characterization. This paper presents a review of the PHA production process with mixed cultures, encompassing the findings reported in the literature as well as our own experimental results in relation to each of these areas.

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Section: Polymer Recovery and Characterizationmentioning

confidence: 99%

Recent Advances in Polyhydroxyalkanoate Production by Mixed Aerobic Cultures: From the Substrate to the Final Product

Dias

Lemos

Serafim

et al. 2006

Macromolecular Bioscience

262

139

View full text Add to dashboard Cite

show abstract

“…Several NPCM solubilizing approaches based on chemicals (sodium hypochlorite) [20]; surfactants (sodium dodecyl sulphate (SDS), palmitoyl carnitine) [9], [37]; surfactant-chelate combination (Triton X-100 and betaine with EDTA) [8], [35]; enzymatic (commercial enzymes such as bromelain, pancreatin; microbial Cytophaga and Microbiospora sp. lysing enzymes [23], [31], [35]; ionic liquids [13]; supercritical fluids [11], [24]; γ-irradation [12]; mechanical aids [12], [17], [25], [54]; dissolved air flotation [56]; osmotic cell fragility [16], [48]; spontaneous liberation [30] etc. to name a few have been reported for PHA recovery from NPCM [34].…”

Section: Introductionmentioning

confidence: 99%

Biolytic extraction of poly(3-hydroxybutyrate) from Bacillus megaterium Ti3 using the lytic enzyme of Streptomyces albus Tia1

Israni

Thapa

Shivakumar

2018

Journal of Genetic Engineering and Biotechnology

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The applicability of Streptomyces sp. cell lytic enzymes for devising a simple and competent biological polyhydroxyalkanoate (PHA) recovery approach from Bacillus megaterium cells was investigated. B. megaterium strain Ti3 produced 50% (w/w) PHA using glucose as carbon source. The intracellular PHA was recovered employing a non-PHA accumulating actinomycetes (Tia1) identified as Streptomyces albus, having potent lytic activity against living and heat inactivated B. megaterium. Interestingly, maximum biomass (2.53 ± 0.6 g/L by 24 h) of the lytic actinomycete was obtained in PHA production medium itself thus circumventing the prior actinomycete acclimatization just by co-inoculation with B. megaterium as an inducer. Maximum lytic activity was observed at pH 6.0, 40 °C, 220 mg of biomass and 33.3 mL of concentrated culture filtrate in a 100 mL reaction mixture. Preliminary biochemical investigations confirmed the proteolytic and caseinolytic nature of the lytic enzyme. PHA yield of 0.55 g/g by co-inoculation extraction approach was comparable with the conventional sodium hypochlorite based extraction method. Interestingly, S. albus also demonstrated a broad spectrum lytic potential against varied Gram-negative and Gram-positive PHA producers highlighting the extensive applicability of this biolytic PHA recovery approach. The lytic enzyme retained almost 100% relative activity on storage at −20 °C upto two months. 1H Nuclear magnetic resonance analysis of the extracted polymer confirmed it as a homopolymer composed of 3-hydroxybutyrate monomeric units. This is the first report on Streptomyces sp. based biological and eco-friendly, intracellular PHA recovery from Bacillus spp.

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“…Other bacterial genera ( Methylobacterium, Pelomonas , and Sphingomonas ) had high relative abundances on fresh tea leaves, which greatly decreased during the Fu Brick manufacturing process. Methylobacterium is known for the production of poly-β-hydroxybutyrate (Bourque et al, 1992 ; Yellore et al, 2000 ; Ghatnekar et al, 2002 ) and was identified as one of the core bacteria during the pile fermentation processing stage (Liu et al, 2019 ). It is also one of the predominant genera of raw Pu'er tea, which is another type of fermented tea derived from the leaves of C. sinensis var.…”

Section: Discussionmentioning

confidence: 99%

Microbial Succession and Interactions During the Manufacture of Fu Brick Tea

et al. 2022

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Fu Brick tea is a very popular post-fermented tea that is known for its “golden flower fungus,” Aspergillus cristatus, which becomes the dominant microbe during the maturation process. This study used both culture-dependent methods and high-throughput sequencing to track microbial succession and interactions during the development of the golden flower fungus, a crucial component of the manufacturing process of Fu Brick tea. Among the bacterial communities, Klebsiella and Lactobacillus were consistently cultured from both fresh tea leaves and in post-fermentation Fu Brick tea. Methylobacterium, Pelomonas, and Sphingomonas were dominant genera in fresh tea leaves but declined once fermentation started, while Bacillus, Kluyvera, and Paenibacillus became dominant after piling fermentation. The abundance of A. cristatus increased during the manufacturing process, accounting for over 98% of all fungi present after the golden flower bloom in the Fu Brick tea product. Despite their consistent presence during culture work, network analysis showed Lactobacillus and Klebsiella to be negatively correlated with A. cristatus. Bacillus spp., as expected from culture work, positively correlated with the presence of golden flower fungus. This study provides complete insights about the succession of microbial communities and highlights the importance of co-occurrence microbes with A. cristatus during the manufacturing process of Fu Brick tea.

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Production and recovery of poly‐3‐hydroxybutyrate from Methylobacterium sp V49

Cited by 63 publications

References 14 publications

Recent Advances in Polyhydroxyalkanoate Production by Mixed Aerobic Cultures: From the Substrate to the Final Product

Recent Advances in Polyhydroxyalkanoate Production by Mixed Aerobic Cultures: From the Substrate to the Final Product

Biolytic extraction of poly(3-hydroxybutyrate) from Bacillus megaterium Ti3 using the lytic enzyme of Streptomyces albus Tia1

Microbial Succession and Interactions During the Manufacture of Fu Brick Tea

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