Production and release of pigments by culture of transformed hairy root of red beet

Taya, Masahito; Mine, Koji; Kino‐oka, Masahiro; Tone, Setsuji; Ichi, Takahito

doi:10.1016/0922-338x(92)90227-l

Cited by 70 publications

(16 citation statements)

References 13 publications

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“…This indicated that the phosphate level in the culture medium correlated with production of target compounds. It was interesting to note that Taya et al [43] reported that betalains production increased by fourfold when hairy roots of Similarly, hairy roots of P. corylifolia produced isoflavones in the absence of phosphate in medium but impaired the growth.…”

Section: Discussionmentioning

confidence: 97%

Impact of nutrient components on production of the phytoestrogens daidzein and genistein by hairy roots of Psoralea corylifolia

2010

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Transformed hairy roots of Psoralea corylifolia were established by infection with Agrobacterium rhizogenes LBA 9402. The aim of this work was to elucidate the effects of media constituents on production of the phytoestrogenic isoflavones daidzein and genistein. A. rhizogenes strain LBA 9402 harboring Ri plasmid was used to transform stem segments of in vitro seedlings. The resultant hairy roots were confirmed by polymerase chain reaction (PCR) and exhibited Ri T-DNA. Transformed hairy root clones were cultured in Murashige and Skoog's (MS) medium altered with different concentrations of NH(4) (+) and NO(3) (-) and their growth and production of isoflavones were assessed. Biomass and productivity increased when MS medium was supplemented with NH(4) (+) and NO(3) (-) at a ratio of 20:10. Increased yield of daidzein was obtained when sucrose level in the culture medium increased, whereas decreased level of sucrose favored genistein production. The hairy roots produced the highest levels of daidzein (2.06% dry wt.) and genistein (0.37% dry wt.) in the presence of low concentrations of PO(4) (3-). Hairy roots secreted trace amounts of daidzein and genistein into the culture medium. The present results demonstrated that the productivity of daidzein was 2.2-fold more than that of untransformed roots.

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Section: Discussionmentioning

confidence: 97%

Impact of nutrient components on production of the phytoestrogens daidzein and genistein by hairy roots of Psoralea corylifolia

2010

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“…For instance, changes in betacyanin/betaxanthin ratios and concentrations of betacyanins have been reported in various betalain-producing plants cultured in in vitro systems [30,31]. Further, flow cytometric analysis has recently shown that hairy root cultures from the beetroot B. vulgaris cv.…”

Section: Cultivation Of Plant Cells and Tissues Under In Vitromentioning

confidence: 97%

Antioxidant Activity and Phenolic Content of Betalain Extracts from Intact Plants and Hairy Root Cultures of the Red Beetroot Beta vulgaris cv. Detroit Dark Red

Georgiev

Weber

Kneschke

et al. 2010

Plant Foods Hum Nutr

357

187

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Betalains are water-soluble plant pigments that are widely used as food colorants, and have a wide range of desirable biological activities, including antioxidant, anti-inflammatory, hepatoprotective, anti-cancer properties. They can be produced from various plants, notably beetroot, but betalain products obtained in this way also have some undesirable properties and are difficult to standardize. A potentially attractive alternative is to use hairy root cultures. In the study reported here, we found that betalain extracts obtained from hairy root cultures of the red beetroot B. vulgaris cv. Detroit Dark Red also had higher antioxidant activity than extracts obtained from mature beetroots: six-fold higher 2,2-dyphenyl-1-picrylhydrazyl radical scavenging ability (90.7% inhibition, EC(50) = 0.11 mg, vs 14.2% inhibition, EC(50) = 0.70 mg) and 3.28-fold higher oxygen radical absorbance capacity (4,100 microM TE/g dry extract, vs 1,250 microM TE/g dry extract). The high antioxidant activity of the hairy root extracts was associated with increased concentrations (more than 20-fold) of total phenolic concomitant compounds, which may have synergistic effects with betalains. The presence of 4-hydroxybenzoic acid, caffeic acid, catechin hydrate, and epicatechin were detected in both types of extract, but at different concentrations. Rutin was only present at high concentration (1.096 mg.g(-1) dry extract) in betalain extracts from the hairy root cultures, whereas chlorogenic acid was only detected at measurable concentrations in extracts from intact plants.

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“…However, later it was realized that large variations occur in conventionally grown beets and the extraction of compounds from fi eld-grown materials has its own set of problems . Ever since hairy roots were reported, red beet was again the material of choice for research (Taya et al 1992(Taya et al , 1994Thimmaraju et al 2003aThimmaraju et al , b, 2004 . Later Pavlov et al ( 2005aPavlov et al ( , b , 2007, Neelwarne and Thimmaraju ( 2009 ) and Suresh et al 2004 ) made substantial contributions towards product formation and down-stream recovery of the betalains, substantiating the commercial feasibility of such processes.…”

Section: Commercial Realities Of Plant In Vitro Culturesmentioning

confidence: 98%

Techno-commercial Aspects of Relevance to Red Beet

Neelwarne

2012

Red Beet Biotechnology

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While Chap. 1 of this book provides an overview of what has been researched in red beet, this chapter tries to put red beet into the newer arena of diverting its current progress towards newer technologies and applications. Beets have unlimited applications, from food, fodder, fuel and pharmaceutical to ornamentals and environmental cleansing agents; this means that the allied technologies span from moderate to very high-tech ventures. Scienti fi cally, as a model system, beet has contributed continuously to our knowledge of cellular physiologies of primary metabolism, energy conservation in the form of sugar synthesis and sequestration processes of primary and secondary metabolites. In the fi eld of genetics, their ever-diversifying nature and amazing adaptability to newer environments have mesmerized scientists. Red beet occupies a position among the top ten vegetables for its antioxidant capacity, cancer-prevention activities and instant blood pressure control. However, it is essential to supplement the current knowledge with innovative scienti fi c methods in terms of validation, characterization and further onward integration of processes through advanced mechanotronics to develop mainstream food products. Treating in vitro cultures and normal beet plant with innovative biotechnologies such as metabolic engineering and genetic modi fi cation is expected to signi fi cantly improve the sustainable production of a wide array of food, pharmaceuticals and bio-energy products. To realize these potentials, the probable technological leaps that are needed are discussed in this chapter.

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Production and release of pigments by culture of transformed hairy root of red beet

Cited by 70 publications

References 13 publications

Impact of nutrient components on production of the phytoestrogens daidzein and genistein by hairy roots of Psoralea corylifolia

Impact of nutrient components on production of the phytoestrogens daidzein and genistein by hairy roots of Psoralea corylifolia

Antioxidant Activity and Phenolic Content of Betalain Extracts from Intact Plants and Hairy Root Cultures of the Red Beetroot Beta vulgaris cv. Detroit Dark Red

Techno-commercial Aspects of Relevance to Red Beet

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