Production and some properties of crude alkaline proteases of indigenous Central Amazonian rhizobia strains

Oliveira, Arlem Nascimento de; Oliveira, Luiz Antonio de; Andrade, Jerusa Souza

doi:10.1590/s1516-89132010000500024

Cited by 8 publications

(7 citation statements)

References 47 publications

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“…Cell wall degrading enzymes like the protease and cellulase are produced by rhizobia which play a role in the degradation of the plant cell wall for infection thread formation and also in antagonism against pathogenic fungi. In congruence with our ndings, protease production has been reported in rhizobia strains by Oliveira et al (2010). Also, Robledo et al (2012) puri ed a cell-bound bacterial cellulase (CelC 2 ) enzyme from Rhizobium leguminosarum bv.…”

Section: Discussionsupporting

confidence: 87%

Ecological performance of multifunctional pesticide tolerant strains of Mesorhizobium sp. in chickpea with recommended pendimethalin, ready-mix of pendimethalin and imazethpyr, carbendazim and chlorpyrifos application

et al. 2022

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The present study was designed to screen the Mesorhizobium strains (50) for tolerance in four recommended pesticides for chickpea. It was followed by in-vitro development of robust pesticide tolerant strains by growth in pesticides amended media over several generations. Further, veri cation of the multifunctional traits of pesticide tolerant mesorhizobia under pesticide stress was conducted invitro. Among different pesticides, signi cantly high tolerance in Mesorhizobium strains was observed with recommended doses of pendimethalin (37%) and ready-mix (36%) followed by chlorpyrifos (31%) and carbendazim (30%), on an overall basis. Based on multifunctional traits, Mesorhizobium strains viz. MR2, MR17 and recommended MR33 were the most promising. Ecological performance of the potential Mesorhizobium strains alone and in dual-inoculation with recommended PGP rhizobacterium strain RB-1 (Pseudomonas argenttinensis JX239745.1) was further analyzed in eld following standard pesticide application in PBG-7 and GPF-2 chickpea varieties for two consecutive rabi seasons (2015 and 2016). Dual-inoculant treatments; recommended RB-1+MR33 (4.1%) and RB-1+MR2 (3.8%) signi cantly increased the grain yield over Mesorhizobium alone treatments viz MR33 and MR2, respectively. Grain yield in PBG7 variety was signi cantly affected (7.3%) by the microbial inoculant treatments over GPF2 variety. Therefore, the potential pesticide tolerant strains MR2 and MR33 can be further explored as compatible dual-inoculants with recommended RB-1 for chickpea under environmentally stressed conditions (pesticide application) at multiple locations. Our approach using robust multifunctional pesticide tolerant Mesorhizobium for bio-augmentation of chickpea with might be helpful in the formulation of effective bio-inoculants consortia in establishing successful chickpea-Mesorhizobium symbiosis.

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Section: Discussionsupporting

confidence: 87%

Ecological performance of multifunctional pesticide tolerant strains of Mesorhizobium sp. in chickpea with recommended pendimethalin, ready-mix of pendimethalin and imazethpyr, carbendazim and chlorpyrifos application

et al. 2022

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“…50 Upon exposure to the moist soil, R. tropici activity increases, and it can be speculated that proteases released by the bacteria and local changes in pH induced by bacterial activity accelerate SF degradation. 51,52 To test this hypothesis, we monitored over time the expression of proteases in R. tropici cultured in PBS and found a time-dependent increase in proteolytic enzyme content in the bacterial culture media (Figure S2a). A one-way ANOVA test was performed and showed a significant difference between the groups, followed by a Tukey test which showed a significant difference (p < 0.05) between day 0 and day 9, as well as between day 3 and day 9.…”

Section: ■ Results and Discussionmentioning

confidence: 99%

“…Upon exposure to the moist soil, R. tropici activity increases, and it can be speculated that proteases released by the bacteria and local changes in pH induced by bacterial activity accelerate SF degradation. , To test this hypothesis, we monitored over time the expression of proteases in R. tropici cultured in PBS and found a time-dependent increase in proteolytic enzyme content in the bacterial culture media (Figure S2a).…”

Section: Resultsmentioning

confidence: 99%

Degradation of Regenerated Silk Fibroin in Soil and Marine Environments

Zvinavashe

Barghouti

Cao

et al. 2022

ACS Sustainable Chem. Eng.

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There is a compelling need to find new materials that meet stringent performance requirements for application in food, water, and agriculture industries while addressing biodegradability, circular life cycle, and sustainable sourcing at scale. Regenerated silk fibroin (SF) is a structural biopolymer with applications in biomedicine, optoelectronics, food, water, and agriculture. Extracted from largely available Bombyx mori cocoons through a water-based process, SF is fabricated into advanced materials that have competitive performance and merits of natural origin and nontoxicity. As a protein, SF is considered slowly degradable in the human body, but as a material, it is known to be environmentally stable, and its biodegradation is mostly unknown. In this study, the degradation of SF in different soil and water environments is investigated. The effects of SF polymorphism, ionic strength, and the presence of microorganisms on proteinaceous material degradation are investigated. Modulation of beta-sheet content allowed us to control the degradation rate of SF films in soil of increasing NaCl concentration. Microbial activity was a key driver for silk degradation under different environmental conditions. Bacterial colonization accelerated silk film degradation, a process that was further enhanced by encapsulation of bacteria in SF materials at the point of material assembly. Together, these data show that SF biodegradation can be controlled by material design and by regulating the interaction with microorganisms present in the environment.

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“…strain R-986 and Bradyrhizobium sp. strain R-993 isolated from the soils of the Central Amazonian floodplain [24]. In addition, De Oliveira et al discovered that maximal protease activities were exhibited when the cell growth reached the stationary phase [24].…”

Section: International Scientific Conference On Microbial Biotechnolo...mentioning

confidence: 99%

Enzymatic activity of nitrogen-fixing bacteria isolated from Armenian saline soils

Toplaghaltsyan,

Karapetyan,

Keleshyan

et al. 2022

5th International Scientific Conference on Microbial Biotechnology

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Nitrogen-fixing bacteria with phytostimulating potential have great interest in the modern world. The aim of this work was to study the enzymatic activity of nitrogen-fixing strains Agrobacterium sp. M-1 and Agrobacterium sp. Y-2. Studies have shown that these strains are capable to produce proteases, lipases, cellulases, amylases and ureases, enzymes that play a vital role in maintaining soil health. In addition, these bacteria have several significant characteristics: salt tolerance, pH stability, maintaining of viability at low and high temperatures. Therefore, the above strains have every chance to become the basis for the creation of a multifunctional biofertilizer in the future

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Production and some properties of crude alkaline proteases of indigenous Central Amazonian rhizobia strains

Abstract: (β-mercaptoethanol), and organic solvents (acetone, hexane, methanol, 1-propanol and toluene).

Cited by 8 publications

References 47 publications

Ecological performance of multifunctional pesticide tolerant strains of Mesorhizobium sp. in chickpea with recommended pendimethalin, ready-mix of pendimethalin and imazethpyr, carbendazim and chlorpyrifos application

Ecological performance of multifunctional pesticide tolerant strains of Mesorhizobium sp. in chickpea with recommended pendimethalin, ready-mix of pendimethalin and imazethpyr, carbendazim and chlorpyrifos application

Degradation of Regenerated Silk Fibroin in Soil and Marine Environments

Enzymatic activity of nitrogen-fixing bacteria isolated from Armenian saline soils

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