Production and structural elucidation of exopolysaccharide from endophytic Pestalotiopsis sp. BC55

Mahapatra, Santanu Kar; Banerjee, Debdulal

doi:10.1016/j.ijbiomac.2015.11.035

Cited by 41 publications

(27 citation statements)

References 33 publications

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“…The strain, A. thailandicum may be a new endophytic fungal genus/species in producing EPS compared to other EPSproducing strains observed in this study. Mahapatra and Banerjee (2016) already reported the EPS production by Pestalotiopsis sp. BC55 isolated from Andrographis paniculata with the highest yield (4.32 g/L) detected during 3.76 days of incubation with glucose as C-source.…”

Section: Resultsmentioning

confidence: 99%

Screening of endophytic fungi from oil palm (Elaeis guineensis) in producing exopolysaccharides

et al. 2021

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Abstract. Yurnaliza Y, Jamilah I, Hartanto A, Lutfia A. 2021. Screening of endophytic fungi from oil palm (Elaeis guineensis) in producing exopolysaccharides. Biodiversitas 22: 1467-1473. Exopolysaccharides (EPS) are microbial polysaccharides with a high biotechnological application in many fields due to their bioactivity and biocompatibility. Endophytic fungi are potential agents for producing EPS even though they remained under-exploited, especially those originating as indigenous strains from oil palm (Elaeis guineensis Jacq.). The present study reports on EPS production by a collection of endophytic fungi isolated from the petioles of oil palm under submerged fermentation. The culture medium was formulated with sucrose as C-source to trigger the secretion of EPS by the strains. Isolation of EPS was done through absolute ethanol precipitation in cold conditions. A total of 29 endophytic fungal strains was recovered from the foliar part of the plants. Molecular identification based on ITS-rDNA region and phylogenetic construction of each lineage revealed the dominant species from Pestalotiopsidaceae (syn. Amphisphaeriaceae), followed by Hypoxylaceae, Pleosporaceae, Didymosphaeriaceae, Sporocadaceae, and other minor families. In general, most isolates were ascomycetous fungi along with a basidiomycetous and zygomycetous fungus. After fermentation for 10 days, three fungal endophytes identified as Annulohypoxylon thailandicum P8 (Hypoxylaceae), Pseudopestalotiopsis simitheae P1 (Pestalotiopsidaceae), and Diaporthe eucalyptorum P11 (Diaporthaceae) secreted EPS higher in values than the others. The most prominent strain was A. thailandicum P8 with a yield of >50 mg/100 mL of filtrate solution. Based on FT-IR spectral analysis, the EPS product was detected to contain carboxyl, carbonyl, glycosidic bonds, and hydroxyl groups hereafter confirmed the characteristics of exopolysaccharides. The results demonstrated that the EPS may be evaluated for its bioactivity in a future study.

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Section: Resultsmentioning

confidence: 99%

Screening of endophytic fungi from oil palm (Elaeis guineensis) in producing exopolysaccharides

et al. 2021

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“…Interestingly the results shown in present study corroborated with EPS (4.320 ± 0.022 g/L) and biomass production from an endophytic fungus Pestalotiopsi s sp. BC55 at optimised conditions [ 35 ]. But in present study the isolate produced relatively high quantity of EPS (4.97 g/L ± 0.25) than those from other reported marine fungi (34, 35).…”

Section: Resultsmentioning

confidence: 99%

Chemical characterization and antioxidant properties of exopolysaccharides from mangrove filamentous fungi Fusarium equiseti ANP2

Prathyusha

Sheela

Bramhachari

2018

Biotechnology Reports

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“…Recently, and Mahapatra and Banerjee (2016) have showed that organic nitrogenous compounds supported higher biomass and EPS yield than the inorganic ones. Supplementation of both inorganic and organic nitrogen in the growth medium at 0.1% (w/v) level showed discrete variation in the growth and polymer production by the isolate B. cereus RCR 08, however maximum EPS production (9.48 g/L) was recorded in presence of ammonium chloride and was followed by organic nitrogenous compounds such as tryptone, casamino acid and beef extract ( Figure 5).…”

Section: Resultsmentioning

confidence: 99%

“…(Wingender et al, 1999) are biosynthesized by bacteria and fungi via intracellular or extracellular processes (Freitas et al, 2011). In recent years, a variety of structurally different EPSs with bioactive potentials have been reported from endophytes (Guo et al, 2014;Mahapatra and Banerjee, 2016;Liu et al, 2017 ).…”

Section: Introductionmentioning

confidence: 99%

Production and partial characterization of extracellular polysaccharide from endophytic Bacillus cereus RCR 08

Mukherjee¹,

Das²,

Sharma³

et al. 2017

Biosci. Biotech. Res. Comm

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The present study focuses attention on the production of extracellular polysaccharide (EPS) by bacterial endophytes of Ricinus communis L. Among the 28 endophytic bacterial isolates screened for EPS production, a potent isolate identifi ed as Bacillus cereus RCR 08 (GenBank accession number MF159112) produced signifi cant amount of EPS in mineral salts medium under batch culture. In single factor system of analysis, glucose and ammonium chloride were most suitable carbon and nitrogen sources respectively for EPS production. Maximum growth (7.1 g/L) and EPS yield (10.24 g/L) was attained when glucose and ammonium chloride were used in the ratio of 25:1. The isolated polymer contained carbohydrate (88.8%), protein (3.18%), RNA (6.0%) as well as DNA (3.2%) and showed characteristic FTIR absorption spectrum with peaks at 3404, 2,933, 1,655, and 1,042 cm −1. The emulsifying activity of the EPS was more or less comparable with Tween 80. Though the EPS failed to show any antibacterial activity, it exhibited moderate DPPH radical scavenging activity and displayed a dose-dependent cytotoxic activity against hepato cellular carcinoma (Huh 7.5) cell line in MTT assay. A detailed physico-chemical analysis is, therefore, essential to assess the signifi cance and potential importance of this endophytic EPS in biotechnology.

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Production and structural elucidation of exopolysaccharide from endophytic Pestalotiopsis sp. BC55

Cited by 41 publications

References 33 publications

Screening of endophytic fungi from oil palm (Elaeis guineensis) in producing exopolysaccharides

Screening of endophytic fungi from oil palm (Elaeis guineensis) in producing exopolysaccharides

Chemical characterization and antioxidant properties of exopolysaccharides from mangrove filamentous fungi Fusarium equiseti ANP2

Production and partial characterization of extracellular polysaccharide from endophytic Bacillus cereus RCR 08

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