Production by quantitative photolithographic synthesis of individually quality checked DNA microarrays

Beier, Markus; Hoheisel, Jörg D.

doi:10.1093/nar/28.4.e11

Cited by 92 publications

(48 citation statements)

References 11 publications

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“…(i) Photolithographic synthesis used by Affymetrix, suitable for making high density microarrays [12], (ii) Maskless Array Synthesis Technology used by Nimblegen and Febit [11] and (iii) inkjet technology used by Rosetta, Agilent, and Oxford Gene Technology [11]. a) Photolithographic synthesis: Here, oligonucleotides are synthesized onto a glass surface due to its optical features and inactivity towards solvents which may be used during the assay [13]. This method of photolithography is adapted from the semiconductor industry for manufacturing silicon chips [14].…”

Section: In-situ Synthesismentioning

confidence: 99%

Microarray Based Genotyping: A Review

2014

J Cancer Sci

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Microarray technology has become a very popular tool in the realm of current molecular biology tools, and combined with the increasing knowledge and accuracy gained by advances in human genome sequencing, this technique is an ideal candidate for performing multiplex high-throughput genetic analysis at a relatively low cost. DNA microarrays are widely used for genotyping and gene expression thus aiding molecular diagnosis. As with any evolving technique, one needs to be careful while interpreting the data so as not to overestimate or underestimate the signals. In this review we discuss the evolution of microarray as a molecular biology tool, the strengths and limitations of most commonly used microarray techniques, and finally we hope to provide the reader with a look into the future of microarray in clinical diagnosis.

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Section: In-situ Synthesismentioning

confidence: 99%

Microarray Based Genotyping: A Review

2014

J Cancer Sci

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“…Standard NPPOC-phosphoramidite chemistry was adopted (Beier and Hoheisel, 2000;Giegrich et al, 1998) for array synthesis. The glass substrate was first clamped in the synthesis chamber, and then the chamber connected to the DNA synthesizer.…”

Section: Array Synthesismentioning

confidence: 99%

Microfluidic ARray Synthesizer (MArS) for rapid preparation and hybridization of custom DNA microarray

Cheng

Chen

2009

Biotech & Bioengineering

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The purpose of this work is to demonstrate a simple method that integrates custom DNA array synthesis and hybridization in a microfluidic channel. Commercial DLP projectors and a classical oligonucleotide synthesizer were integrated with a microfluidic chamber and constitute a Microfluidic ARray Synthesizer (MArS). Utilizing the benefit of microfluidics, the MArS produces two custom oligonucleotide arrays with reagent consumption for one array. The quality of the two arrays was examined by hybridization and shown to be almost identical. Post-synthesis deprotection of the array and hybridization performed in the same microfluidic chamber reduced overall DNA assay time by two orders. In addition, mismatch discrimination was enhanced using the microfluidic chamber. In summary, a simple method to build a custom array synthesizer is demonstrated and its performance is examined.

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“…Four individually accessible microchannels (referred to as arrays), etched into the silica layer of the DNA-processor, were connected to the microfluidic system of the geniom device. Using standard DNA-synthesis reagents and 3'-phosphoramidites with a photolabile protecting group (Hasan et al 1997;Beier and Hoheisel, 2000), oligonucleotides were synthesized in parallel in all four translucent arrays of one reaction carrier. Prior to synthesis, the glass surface was activated by coating with a silane-bound spacer.…”

Section: Oligonucleotide Probe Selectionmentioning

confidence: 99%