Production, characterization and kinetic model of biosurfactant produced by lactic acid bacteria

Vallejo, Carolina Montoya; Restrepo, María Alejandra Flórez; Duque, Fernando León Guzmán; Díaz, José Alfredo Jáuregui

doi:10.1016/j.ejbt.2021.06.001

Cited by 21 publications

(15 citation statements)

References 34 publications

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“…Once produced, biosurfactants can be excreted to the medium [23]. By considering that biosurfactants favor cellular membrane permeability, this manuscript was conceived to evaluate the presence of Lactobacillus plantarum, a lactic acid bacteria, which has been previously evaluated for its ability to produce biosurfactants which do not inhibit E. coli growth [24].…”

Section: Resultsmentioning

confidence: 99%

Enhancement of Electricity Production in Microbial Fuel Cells Using a Biosurfactant-Producing Co-Culture

Montoya-Vallejo,

Gil Posada,

Quintero-Díaz

2023

Molecules

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Microbial fuel cells are bio-electrochemical devices that enable the conversion of chemical energy into bioelectricity. In this manuscript, the use of biosurfactants (Tween 80 and surfactin) and the effect of coculturing E. coli and L. plantarum were used to investigate the generation of bioelectricity coming from an H-type microbial fuel cell. In this setup, E. coli acts as an electron donor while L. plantarum acts as an in situ biosurfactant producer. It was observed that the use of exogenous surfactants enhanced electricity production compared to conventional E. coli cultures. The utilization of Tween 80 and surfactin increased the power generation from 204 µW m−2 to 506 µW m−2 and 577 µW m−2, respectively. Furthermore, co-culturing E. coli and L. plantarum also resulted in a higher power output compared to pure cultures (132.8% more when compared to using E. coli alone and 68.1% more when compared to using L. plantarum alone). Due to the presence of surfactants, the internal resistance of the cell was reduced. The experimental evidence collected here clearly indicates that the production of endogenous surfactants, as well as the addition of exogenous surfactants, will enhance MFC electricity production.

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Section: Resultsmentioning

confidence: 99%

Enhancement of Electricity Production in Microbial Fuel Cells Using a Biosurfactant-Producing Co-Culture

Montoya-Vallejo,

Gil Posada,

Quintero-Díaz

2023

Molecules

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“…The BS production is governed by several factors; medium components (carbon and nitrogen), salt concentrations, pH, temperature, agitation speed, and aeration (Montoya Vallejo et al, 2021). The OFAT strategy was applied in optimization of carbon, nitrogen sources, temperature, agitation, and inoculum size for BS production (Figures 5 and 6).…”

Section: Resultsmentioning

confidence: 99%

Bioprocess optimization for glycopeptide biosurfactant production by means of Lactobacillus delbrueckii: Design expert laden approach

Goyal

Singh²

2022

Food Processing Preservation

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A bacterial strain, Lactobacillus delbrueckii, was isolated and screened for high potential of biosurfactant (BS) production yield. The current research emphasizes the optimization of process parameters through OFAT (one factor at a time) and RSM (response surface methodology) to achieve optimal BS production. Therefore, xylose and peptone were screened as the best carbon and nitrogen sources respectively by means of OFAT, along with temperature (37°C), inoculum size (5%), and fermentation time (39 h). Furthermore, optimization of xylose (1% w/v), peptone (0.5%w/v), and pH (6.5) through Box–Behnken statistical design was validated by ANOVA (analysis of variance) and found significant (p < .05) with optimal BS yield (7.31 g/L). The solvent‐extracted glycopeptide BS displayed cationic nature, with thermal (0–120°C), pH (2–12), and NaCl (2%–12% w/v) stability. The functional attributes of BS like antiadhesive and antimicrobial activity toward Gram‐positive and Gram‐negative bacteria and emulsifying potency against various vegetable oils make it worthwhile and applicable. Practical applications The recent applications of BS and hazards of pathogenic biofilm make it challenging to produce enhanced yield of BS. In light of this, present work is focused to optimize yield from Lactobacillus delbrueckii for its applications as antibiofilm and bioemulsifier agent in various industries. Furthermore, the glycoproteinaceous BS from microbe in question is itself unique and novel. Additionally, the statistical design of optimization provides experiment modeling, production rate, and significance of opted design which makes the whole process feasible to explore new possibilities for BS production.

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“…(rhamnolipids) [35,36,37]. For the other microorganisms, almost researches performed on biosurfactants emphasized their functional groups characterization [1,2,3,38,39,40]. These biosurfactants have yet to be fully characterized at structural level.…”

Section: Severalmentioning

confidence: 99%

“…including bacteria, molds and yeasts [1,2]. They are either excreted extracellularly or remain bound to the producing cells [3]. They are synthetized by microorganisms through different metabolism routes depending of the nature and the composition of the substrates (Fig.…”

Section: Introductionmentioning

confidence: 99%

Methods of Purification and Characterization of Biosurfactants: An Overview

Mouafo

Pahane

Arsène

et al. 2023

JABB

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Biosurfactants are amphiphilic molecules produced by several microorganisms including bacteria, filamentous fungi and yeasts. They are classified according to their chemical composition (glycolipids, lipopeptides, glycoproteins, glycolipopeptides, phospholipids) and their molecular weight (low and high molecular weight). Biosurfactants are currently used in several industrial fields (food industry, pharmacy, medicine, detergents, agriculture, cosmetics, oil recovery and bioremediation) owning to some of their specific properties such as their low toxicity, biocompatibility, biodegradability, environmentally-friendly, stability under extreme conditions (temperature, pH and salinity), structural diversity, production using renewable low-cost substrates, agreement with green chemistry and sustainability. Despite the properties of biosurfactants, there are few studies leading to their structural characterization. The most reported structures are from biosurfactants produced by Bacillus spp. (surfactins, fengycins, lichenysines) and Pseudomonas spp. (rhamnolipids). The majority of researches performed on biosurfactants emphasized their functional groups characterization. These biosurfactants have yet to be fully characterized at structural level. Hence, in this review, we highlight the different purification and structural characterization techniques which can be combined to provide information on the structure of the biosurfactants independently of its complexity. This will lead to enhance their application in some highly pointed industrial fields such as medicine and pharmacy.

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Production, characterization and kinetic model of biosurfactant produced by lactic acid bacteria

Cited by 21 publications

References 34 publications

Enhancement of Electricity Production in Microbial Fuel Cells Using a Biosurfactant-Producing Co-Culture

Enhancement of Electricity Production in Microbial Fuel Cells Using a Biosurfactant-Producing Co-Culture

Bioprocess optimization for glycopeptide biosurfactant production by means of Lactobacillus delbrueckii: Design expert laden approach

Methods of Purification and Characterization of Biosurfactants: An Overview

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