Production, characterization and reactivity of monoclonal antibodies to porcine reproductive and respiratory syndrome virus

Drew, Trevor W.; Meulenberg, J.J.M.; Sands, J. J.; Paton, David

doi:10.1099/0022-1317-76-6-1361

Cited by 101 publications

(78 citation statements)

References 22 publications

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“…On the other hand, the antigenic variability of PRRS virus was recognized by monoclonal antibodies [2,16] and field isolates of PRRS virus varied in their susceptibility to ADE [30]. Thus the antigenic change in PRRS virus under the presence of specific antibody should be taken into consideration in the phenomenon of prolonged duration of viremia in the conventional pigs.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Experimental Infection of Maternally Immune Pigs with Porcine Reproductive and Respiratory Syndrome(PRRS) Virus.

Shibata

Mori

Uruno

1998

The Journal of Veterinary Medical Science

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ABSTRACT. To investigate the influence of maternal antibody to porcine reproductive and respiratory syndrome (PRRS) virus infection, the following examination was done using conventional and SPF pigs. Ten 17-day-old conventional pigs with maternal antibody against PRRS virus and 6 44-day-old SPF pigs seronegative were inoculated intranasally with 10 5.0 TCID 50 of PRRS virus. Two conventional and 4 SPF pigs were served as non-inoculated control. In conventional pigs, coughing and febrile response were observed after inoculation, and mean rate of weight gain reduced. One of the inoculated conventional pigs died on post-inoculation-day (PID) 28 and Haemophilus parasuis was isolated from the lung. Although febrile response was also observed in the inoculated SPF pigs, reduction in weight gain rate was not recognized. Virus was isolated from all the sera of inoculated conventional and SPF pigs except one conventional pig between PID 7 and 49, and between PID 7 and 28, respectively. Onset of viremia in the several conventional pigs delayed. Virus was isolated from the tissues of the 5 conventional pigs on PID 65 and from the tissues of the dead pig. On the other hand, virus was not isolated from the tissues of non-inoculated conventional pigs, and inoculated and non-inoculated SPF pigs. At the virus inoculation, antibodies by the indirect fluorescent antibody (IFA) assay against PRRS virus were detected in the sera of conventional pigs with antibody titers of 1:20. Antibody titers gradually decreased after inoculation and rose from PID 21 or 28 and were between 1:160 and 1:640 on PID 63. Virus neutralization (VN) antibody titers were 1:2 or 1:4 at the inoculation and gradually decreased. Apparent rise in VN antibody titer was not observed after the inoculation. In the sera of control pigs, both antibody titers gradually decreased and did not rise. In the sera of the SPF pigs, antibodies by the IFA assay were first detected on PID 7 or 14. The titers of antibodies rose and reached their maximum with 1:320 to 1:2,560 on PID 21 to 35. VN antibodies were first detected on PID 42 to 56 and thereafter, the titers ranged between 1:1 to 1:4. Control SPF pigs were free of antibody throughout the examination. Antigenic variability was not recognized between the inoculated and recovered viruses by the VN test. The prolonged duration of viremia and virus isolation from the tissues on PID 65 in conventional pigs with low maternal antibody might support the present of antibody-dependent enhancement activity of PRRS virus infection. -KEY WORDS: antibody-dependent enhancement, experimental infection, maternal antibody, PRRS virus, swine.

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Section: Discussionmentioning

confidence: 99%

“…SAM are the predominant cells for PRRS virus replication in vivo [18,21]. Antigenic variations among PRRS virus isolates have been demonstrated by serological assays using polyclonal [26] and monoclonal antibodies [2,16].…”

Section: Methodsmentioning

confidence: 99%

Experimental Infection of Maternally Immune Pigs with Porcine Reproductive and Respiratory Syndrome(PRRS) Virus.

Shibata

Mori

Uruno

1998

The Journal of Veterinary Medical Science

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“…Briefly, cytocentrifuge preparations and cryostat sections, fixed in 4% paraformaldehyde, were treated with Triton X-100 (0.1%) at 4°C for 2 min. The preparations were first incubated with a pool of monoclonal antibodies (MAb) against the PRRSV nucleocapsid protein (dilution 1/100 of WBE1 and WBE4-6) [9]. Subsequently, the preparations were subjected to an enzymatic incorporation of digoxygenin-labelled nucleotide with TdT and then incubated with goat anti-mouse TexasRed (dilution 1/100) (Amersham Biosciences, Buckinghamshire, United Kingdom).…”

Section: Double-labelling Experimentsmentioning

confidence: 99%

Apoptosis in the lungs of pigs infected with porcine reproductive and respiratory syndrome virus and associations with the production of apoptogenic cytokines

et al. 2003

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-Apoptosis was studied in the lungs of pigs during an infection with a European strain of porcine reproductive and respiratory syndrome virus (PRRSV) and it was examined if cytokines were involved in the induction of apoptosis. Twenty-two 4-to 5-week-old gnotobiotic pigs were inoculated intranasally with 10 6.0 TCID 50 of the Lelystad virus and euthanised between 1 and 52 days post inoculation (PI). The lungs and broncho-alveolar lavage (BAL) cells were assessed both for virus replication and apoptosis; BAL fluids were examined for interleukin (IL)-1, tumour necrosis factor-alpha and IL-10. Double-labellings were conducted to determine the relation between virus replication and apoptosis and to identify the apoptotic cells. Apoptosis occurred in both infected and non-infected cells. The percentages of infected cells, which were apoptotic, ranged between 9 and 39% in the lungs and between 13 and 30% in the BAL cells. The majority of apoptotic cells were non-infected. Non-infected apoptotic cells in the lungs were predominantly monocytes/macrophages, whereas those in the broncho-alveolar spaces were predominantly lymphocytes. The peak of apoptosis in the lungs at 14 days PI was preceded by a peak of IL-1 and IL-10 production at 9 days PI, suggesting a possible role of these cytokines in the induction of apoptosis in non-infected interstitial monocytes/macrophages. However, the latter hypothesis was not confirmed in vitro, since blood monocytes or alveolar macrophages did not undergo apoptosis after treatment with recombinant porcine IL-1 or IL-10. pig / lung / porcine reproductive and respiratory syndrome virus / apoptosis / cytokine

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“…To assess the accuracy of the TaqMan ® assay in differentiating EAV from other arteriviruses, a series of 10-fold dilutions of viral RNA were carried out on the isolate H2 (Humberside) of porcine reproductive and respiratory syndrome virus (PRRSV) [5]. In addition, DNA (Qiagen, Crawley, UK) from equine herpesvirus-1 (RACH strain) and a field isolate of equine herpesvirus-2 were tested by the TaqMan ® assay.…”

Section: Virus Detection By Virus Isolation (Vi)mentioning

confidence: 99%

Use of an internal standard in a closed one-tube RT-PCR for the detection of equine arteritis virus RNA with fluorescent probes

et al. 2003

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-Routine detection of equine arteritis virus (EAV) can be achieved by virus isolation (VI) in cell culture, or by the amplification of viral genome by molecular methods. To simplify molecular diagnosis, a number of different Reverse Transcriptase Polymerase Chain Reaction (RT-PCR) and RT-nested PCR (RT-nPCR) assays were compared, and a one-tube method was developed and optimised utilizing a fluorogenic probe (TaqMan ® ). An artificial RNA template (Mimic) and associated probe were also constructed to provide in-tube validation of the RT-PCR system. To assess the utility of the RT-PCR TaqMan ® assay, 28 different isolates of EAV representing different genetic groups of American and European strains were tested. Furthermore, the ability of VI and RT-PCR TaqMan ® assay to detect EAV in different biological matrices such as semen, nasal and faecal swabs and blood was compared. All 28 EAV strains were detected by the RT-PCR TaqMan ® assay. The results of TaqMan ® and VI testing were in agreement for 30 of the 33 semen samples and all of the 50 other clinical specimens examined: the RT-PCR TaqMan ® assay detected 18 positive semen samples, three more than VI. In conclusion, the one-tube RT-PCR TaqMan ® assay is a rapid, reliable method for the detection of EAV.equine arteritis virus / RT-PCR / mimic / fluorescent probe / TaqMan ®

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Production, characterization and reactivity of monoclonal antibodies to porcine reproductive and respiratory syndrome virus

Cited by 101 publications

References 22 publications

Experimental Infection of Maternally Immune Pigs with Porcine Reproductive and Respiratory Syndrome(PRRS) Virus.

Experimental Infection of Maternally Immune Pigs with Porcine Reproductive and Respiratory Syndrome(PRRS) Virus.

Apoptosis in the lungs of pigs infected with porcine reproductive and respiratory syndrome virus and associations with the production of apoptogenic cytokines

Use of an internal standard in a closed one-tube RT-PCR for the detection of equine arteritis virus RNA with fluorescent probes

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