Production of 2,3-butanediol by Enterobacter cloacae from corncob-derived xylose

Zhang, Cuiying; Li, Wei; Wang, Dongsheng; Guo, Xuewu; Ma, Lijuan; Xiao, Dongguang

doi:10.3906/biy-1506-66

Cited by 9 publications

(8 citation statements)

References 32 publications

(44 reference statements)

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“…The highest BDO titer of 81.4 g/L using xylose solution from corncob hydrolysate and corn liquor (20 g/L) has been reported by E. cloacae with a conversion yield of 0.39 g/g and a productivity of 0.72 g/L·h . Unlike our work where forced pH fluctuation was employed, the pH was controlled in the case of other bacterial strains.…”

Section: Discussionmentioning

confidence: 81%

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Integrated Fermentative Production and Downstream Processing of 2,3-Butanediol from Sugarcane Bagasse-Derived Xylose by Mutant Strain of Enterobacter ludwigii

Amraoui

Narisetty

Coulon

et al. 2021

ACS Sustainable Chem. Eng.

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In this study, a mutant strain of Enterobacter ludwigii developed in our previous work, was evaluated to utilize pure xylose as the sole carbon and energy source for 2,3-butanediol (BDO) production. Later, this strain was also investigated on detoxified and nondetoxified xylose-rich hydrolysate obtained from hydrothermally pretreated sugarcane bagasse (SCB) for BDO production. Supplementing the fermentation medium with 0.2% w/v yeast extract improved cell growth (31%), BDO titer (43%), and yield (41%) against the synthetic medium devoid of any complex nitrogen source. The fed-batch culture with cyclic control of pH resulted in a BDO production of 71.1 g/L from pure xylose with overall yield and productivity of 0.40 g/g and 0.94 g/L·h, respectively. While BDO titer, yield, and productivity of 63.5 g/L, 0.36 g/g, and 0.84 g/L·h, were acheived with detoxified hydrolysate, respectively. In contrast, 32.7 g/L BDO was produced from nondetoxified hydrolysate with a conversion yield of 0.33 g/g and a productivity of 0.43 g/L·h. BDO accumulated on pure xylose and detoxified SCB hydrolysate was separated by aqueous two-phase system (ATPS) method using (NH4)2SO4 as salting-out agent and isopropanol as an extractant, resulting in the BDO recovery of more than 85%. The results achieved in the current work exemplify a step toward industrial BDO production from cost-effective hemicellulosic hydrolysates by E. ludwigii.

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Section: Discussionmentioning

confidence: 81%

“…Summary of Microbial BDO Production from Pure/Crude Xylose /g and a productivity of 0.72 g/L•h 31. Unlike our work where forced pH fluctuation was employed, the pH was controlled in the case of other bacterial strains.…”

mentioning

confidence: 97%

Integrated Fermentative Production and Downstream Processing of 2,3-Butanediol from Sugarcane Bagasse-Derived Xylose by Mutant Strain of Enterobacter ludwigii

Amraoui

Narisetty

Coulon

et al. 2021

ACS Sustainable Chem. Eng.

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“…2,3-Butanediol (2,3-BDO) is an important platform compound with applications in aerospace, food, pharmaceuticals, chemicals, and fuels (Priya and Lal 2019). Along with the increasing problems of crude oil scarcity, rising fossil fuel prices, and the everincreasing-environmental pollution, the synthesis of 2,3-BDO by biological methods has regained widespread attention (Zhang et al 2016). The main substrates used for the biosynthesis of 2,3-BDO are glucose and glycerol.…”

Section: Introductionmentioning

confidence: 99%

Utilization of corncob hydrolysate enables 2,3-butanediol production in Enterobacter cholerae

Wang

Zhi

et al. 2022

BioRes

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2,3-Butanediol (2,3-BDO) is an important industrial diol that could function in various fields. Currently, there are many substrates used for 2,3-BDO biosynthesis, but studies using green carbon sources such as corncob hydrolysate as a substrate are lacking. As a widely distributed waste lignocellulose-derived substrate, corncob hydrolysate is nutrient-rich and cost-effective. The present study evaluated 2,3-BDO production via an Enterobacter cholerae strain using corncob hydrolysate as carbon source. Chemical component analysis showed that concentrated corncob hydrolysate contained 233 g/L total sugar and showed no inhibitory effect, but it was beneficial for 2,3-BDO synthesis. Optimization experiments for fermentation resulted in a titer of 47.23 g/L 2,3-BDO with a yield of 0.30 g/g and a productivity rate of 0.66 g/L•h. This study is expected to provide insights for large-scale bioproduction of bulk chemicals utilizing corncob hydrolysates.

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“…The 2,3-BD industry has been more popular as of recent because of the increased research on 2,3-BD conversion from renewable sugars and agricultural wastes. − Previous studies have shown that high titers of 78.9 g/L 2,3-BD can be produced from Klebsiella pneumoniae and 81.4 g/L from Enterobacter cloacae . However, these organisms are classified as risk 2 and pose certain health concerns .…”

Section: Introductionmentioning

confidence: 99%

“…8−11 Previous studies have shown that high titers of 78.9 g/L 2,3-BD can be produced from Klebsiella pneumoniae 12 and 81.4 g/L from Enterobacter cloacae. 13 However, these organisms are classified as risk 2 and pose certain health concerns. 14 Organisms generally regarded as safe (GRAS) pose less risk and include several common Bacillus species.…”

Section: ■ Introductionmentioning

confidence: 99%

Thermophilic and Alkaliphilic Bacillus licheniformis YNP5-TSU as an Ideal Candidate for 2,3-Butanediol Production

O’Hair

Jin

et al. 2020

ACS Sustainable Chem. Eng.

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2,3-Butanediol (2,3-BD) is an important platform chemical that can be produced biologically. It is currently fermented below 40 °C using mesophilic strains at acid to neutral pH conditions, but the processes often suffer bacterial contamination. Here we reported a new nonpathogenic strain Bacillus licheniformis YNP5-TSU isolated from Yellowstone National Park, which produces 2,3-BD with high yield and productivity at thermophilic and alkaline conditions. B. licheniformis YNP5-TSU has the ability to survive in pH ranges from 8.0 to 10.0 and temperature ranges from 40 to 55 °C, which greatly reduces the chance of contamination from other microorganisms. At its optimum condition with a temperature of 50 °C and pH of 8− 9, YNP5-TSU can produce 27.4 g/L 2,3-BD from 60 g/L glucose, with a high yield of 0.46 g/g (92% of theoretical value) and productivity of 1.1 g/L/h in a batch fermentation. YNP5-TSU also can ferment different carbohydrates including fructose, sucrose, arabinose, galactose, mannose, xylose, as well as alcohols such as glycerol. The effects of nitrogen and mineral sources on 2,3-BD fermentation were also investigated to improve the fermentation process. In a fed-batch fermentation, 99.3 g/L of 2,3-BD was obtained within 96 h, with a yield of 0.41 g/g. The results provided indicate that B. licheniformis YNP5-TSU is a very adaptable strain to withstand harsh environments while still producing 2,3-BD near theoretical values.

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Production of 2,3-butanediol by Enterobacter cloacae from corncob-derived xylose

Cited by 9 publications

References 32 publications

Integrated Fermentative Production and Downstream Processing of 2,3-Butanediol from Sugarcane Bagasse-Derived Xylose by Mutant Strain of Enterobacter ludwigii

Integrated Fermentative Production and Downstream Processing of 2,3-Butanediol from Sugarcane Bagasse-Derived Xylose by Mutant Strain of Enterobacter ludwigii

Utilization of corncob hydrolysate enables 2,3-butanediol production in Enterobacter cholerae

Thermophilic and Alkaliphilic Bacillus licheniformis YNP5-TSU as an Ideal Candidate for 2,3-Butanediol Production

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