Production of 2,3-butanediol from glucose byBacillus licheniformis

Nilegaonkar, Smita; Bhosale, Suresh B.; Kshirsagar, Deepa; Kapadi, A. H.

doi:10.1007/bf01198748

Cited by 36 publications

(22 citation statements)

References 15 publications

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“…Then, the operating conditions for 2,3-BD production from glucose by strain 10-1-A was also optimized. It was reported that pH 6.0 was the optimal condition for production of 2,3-BD by B. licheniformis [31]. For B. licheniformis BL5, pH 5.0 was regarded as the optimum pH for 2,3-BD production [15].…”

Section: Discussionmentioning

confidence: 99%

A newly isolated Bacillus licheniformisstrain thermophilically produces 2,3-butanediol, a platform and fuel bio-chemical

Zhang

et al. 2013

Biotechnol Biofuels

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Background2,3-Butanediol (2,3-BD), a platform and fuel bio-chemical, can be efficiently produced by Klebsiella pneumonia, K. oxytoca, and Serratia marcescens. However, these strains are opportunistic pathogens and not favorable for industrial application. Although some generally regarded as safe (GRAS) microorganisms have been isolated in recent years, there is still a demand for safe 2,3-BD producing strains with high productivity and yield under thermophilic fermentation.ResultsBacillus licheniformis strain 10-1-A was newly isolated for 2,3-BD production. The optimum temperature and medium pH were 50°C and pH 7.0 for 2,3-BD production by strain 10-1-A. The medium composition was optimized through Plackett–Burman design and response surface methodology techniques. With a two-stage agitation speed control strategy, 115.7 g/L of 2,3-BD was obtained from glucose by fed-batch fermentation in a 5-L bioreactor with a high productivity (2.4 g/L·h) and yield (94% of its theoretical value). The 2,3-BD produced by strain 10-1-A comprises (2R,3R)-2,3-BD and meso-2,3-BD with a ratio of nearly 1:1. The bdh and gdh genes encoding meso-2,3-butanediol dehydrogenase (meso-BDH) and glycerol dehydrogenase (GDH) of strain 10-1-A were expressed in Escherichia coli and the proteins were purified. meso-2,3-BD and (2R,3R)-2,3-BD were transformed from racemic acetoin by meso-BDH and GDH with NADH, respectively.ConclusionsCompared with the reported GRAS 2,3-BD producers, B. licheniformis 10-1-A could thermophilically produce 2,3-BD with a high concentration, productivity and yield. Thus, the newly isolated GRAS strain 10-1-A might be a promising strain for industrial production of 2,3-BD. Two key enzymes for meso-2,3-BD and (2R,3R)-2,3-BD production were purified and further studied, and this might be helpful to understand the mechanism for 2,3-BD stereoisomers forming in B. licheniformis.

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Section: Discussionmentioning

confidence: 99%

A newly isolated Bacillus licheniformisstrain thermophilically produces 2,3-butanediol, a platform and fuel bio-chemical

Zhang

et al. 2013

Biotechnol Biofuels

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“…Like most microbial processes, 2,3-BD biosynthesis is enzyme-controlled, therefore, fermentation temperature impacts substrate consumption and 2,3-BD production, because enzyme activity is temperature-dependent [19,20]. Inoculum size has been reported to increase substrate utilization with improved 2,3-BD production and yield [19,27]. To our knowledge, this is the first report of combined optimization of fermentation nutrients and conditions for enhanced 2,3-BD production by P. polymyxa.…”

Section: Plackett-burman Designmentioning

confidence: 99%

Process Development for Enhanced 2,3-Butanediol Production by Paenibacillus polymyxa DSM 365

et al. 2017

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While chiral 2,3-Butanediol (2,3-BD) is currently receiving remarkable attention because of its numerous industrial applications in the synthetic rubber, bioplastics, cosmetics, and flavor industries, 2,3-BD-mediated feedback inhibition of Paenibacillus polymyxa DSM 365 limits the accumulation of higher concentrations of 2,3-BD in the bioreactor during fermentation. The Box-Behnken design, Plackett-Burman design (PBD), and response surface methodology were employed to evaluate the impacts of seven factors including tryptone, yeast extract, ammonium acetate, ammonium sulfate, glycerol concentrations, temperature, and inoculum size on 2,3-butanediol (2,3-BD) production by Paenibacillus polymyxa DSM 365. Results showed that three factors; tryptone, temperature, and inoculum size significantly influence 2,3-BD production (p < 0.05) by P. polymyxa. The optimal levels of tryptone, inoculum size, and temperature as determined by the Box-Behnken design and response surface methodology were 3.5 g/L, 9.5%, and 35 • C, respectively. The optimized process was validated in batch and fed-batch fermentations in a 5-L Bioflo 3000 Bioreactor, and 51.10 and 68.54 g/L 2,3-BD were obtained, respectively. Interestingly, the production of exopolysaccharides (EPS), an undesirable co-product, was reduced by 19% when compared to the control. These results underscore an interplay between medium components and fermentation conditions, leading to increased 2,3-BD production and decreased EPS production by P. polymyxa. Collectively, our findings demonstrate both increased 2,3-BD titer, a fundamental prerequisite to the potential commercialization of fermentative 2,3-BD production using renewable feedstocks, and reduced flux of carbons towards undesirable EPS production.

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“…Although the aeration conditions for this fermentation have been deeply studied by Zeng et al in terms of respiratory quotient and oxygen transfer rate [9±13], a complete optimisation study of the remaining operating parameters, like those available for other butanediol producers [3,14], could be the key of future improvements of both product yield and productivity. The necessity of these advances is demonstrated by the fact that low product concentrations in the broth, besides the additional problems of high boiling point and hydroscopicity [3,4], implies high recovery costs.…”

Section: List Of Symbolsmentioning

confidence: 99%

2,3-Butanediol production by Enterobacter aerogenes: selection of the optimal conditions and application to food industry residues

Perego

Converti

Borghi

et al. 2000

Bioprocess Engineering

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Production of 2,3-butanediol from glucose byBacillus licheniformis

Cited by 36 publications

References 15 publications

A newly isolated Bacillus licheniformisstrain thermophilically produces 2,3-butanediol, a platform and fuel bio-chemical

A newly isolated Bacillus licheniformisstrain thermophilically produces 2,3-butanediol, a platform and fuel bio-chemical

Process Development for Enhanced 2,3-Butanediol Production by Paenibacillus polymyxa DSM 365

2,3-Butanediol production by Enterobacter aerogenes: selection of the optimal conditions and application to food industry residues

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