Production of 3-Hydroxypropionaldehyde in Silage Inoculated with<i>Lactobacillus coryniformis</i>Plus Glycerol

Twenty-three lactic acid bacteria (LAB) isolated from three cultivars (Akiaoba, Nagahahikari and Tachiwase) of Italian ryegrass (Lolium multiflorum Lam.) silage were precisely characterized by a combination of phenotypic tests, genotypic 16S ribosomal DNA sequencing and rapid PCR-based analyses, focusing on their useful phenotypes for silage preparation as inoculants. We successfully identified both at the species and subspecies levels: phenotypically novel Lactococcus lactis subsp. lactis, Lactobacillus brevis, Lactobacillus coryniformis subsp. torquens, Lactobacillus curvatus, Lactobacillus plantarum subsp. plantarum, Lactobacillus sakei subsp. carnosus, Leuconostoc mesenteroides subsp. dextranicum and Pediococcus parvulus. This is the first report to elucidate the presence of Lactobacillus coryniformis ssp. torquens and Leuconostoc mesenteroides subsp. dextranicum in Italian ryegrass silages. Physiological and biochemical tests revealed that phenotypic characteristics are different among the different strains of the same species and subspecies, and that the isolates show unique and diverse phenotypes related to fermentation factors, such as available carbohydrates, optimal growth pH and temperature. These results suggest that, for various well-preserved silage preparations, the isolates may be useful in producing novel inoculants corresponding to their optimally climatic and ecological niches.

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“…1992; Magnusson & Schnurer 2001; Schachtsiek et al . 2004; Tanaka et al . 2009), its subspecies characterizaton has not been well understood.…”

Section: Resultsmentioning

confidence: 99%

Genotypic and phenotypic characterization of lactic acid bacteria isolated from Italian ryegrass silage

Tohno

Kobayashi

Nomura

et al. 2011

Animal Science Journal

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“…There are no reports on the toxicity of glycerol to yeasts. However, the metabolism of glycerol by LAB results in the production of 3‐hydroxypropionaldehyde (3‐HPA), which is an antimicrobial compound known as reuterin (Pasteris and Strasser de Saad, ; Tanaka et al ., ). According to eukaryotic diversity analysis (Figure b), the likely inhibitory effect of these compounds acted selectively with respect to yeast species, since a small proportion in the population of Candida humilis and Issatchenkia orientalis were observed in the 12% glycerine treatment (Figure b).…”

Section: Discussionmentioning

confidence: 97%

Methylotrophic yeast, lactic acid bacteria and glycerine as additives for sugarcane silage

Carvalho

Ávila

Pereira

et al. 2016

Grass and Forage Science

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The ensiling of sugarcane results in high dry‐matter (DM) loss, but the addition of glycerine may compensate for the loss during ensiling. Methanol is the most undesirable contaminant of crude glycerine destined for animal feeding. The aim of this study was to evaluate the ability of the yeast strain Pichia methanolica NCYC 1381 to reduce the methanol concentration in sugarcane silage inoculated with Lactobacillus hilgardii CCMA 0170 + glycerine. A randomized design consisted of four dose rates of glycerine (0, 4, 8 and 12% of fresh forage), three periods of silage fermentation (11, 34 and 68 d) and three combinations of microbial additives [L. hilgardii (LH), L. hilgardii plus P. methanolica (LH + PM) and without microbial additive (WI)]. The DM of the fresh sugarcane was 275 g kg−1. The linear reduction in neutral detergent fibre caused by glycerine inclusion was probably due to a dilution effect. The LH treatment increased the concentrations of the succinic, acetic and propionic acids, and 1,2‐propanediol, and reduced the yeast population. The LH + PM treatment increased DM loss of sugarcane silage with 12% glycerine and L. hilgardii CCMA 0170 (6·1 log cfu g−1 of FM) reduced the DM loss when compared to the silage without additives. Under the conditions of the experiment, the P. methanolica treatment did not reduce the methanol concentration in silage.

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“…Other isolates of homofermentative rod-shaped strains were identified as L. coryniformis, L. acidipiscis, L. sakei, L. curvatus and C. divergens. L. coryniformis has often been found on plant material and in various silages (Masuko et al 1992;Magnusson & Schnurer 2001;Schachtsiek et al 2004;Tanaka et al 2009). We successfully distinguished the closely related subspecies of L. coryniformis in the silages, indicating that both subsp.…”

Section: Discussionmentioning

confidence: 99%

Identification and characterization of lactic acid bacteria isolated from mixed pasture of timothy and orchardgrass, and its badly preserved silage

Tohno

Kobayashi

Nomura

et al. 2011

Animal Science Journal

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In order to understand the relationship between lactic acid bacteria (LAB) species and silage fermentation, a total of 65 LAB strains isolated from mixed pasture of timothy (Phleum pratense L.) and orchardgrass (Dactylis glomerata L.), and its badly preserved silages were subjected to phenotypic and genetic analysis. According to these analyses, the isolates were divided into 13 groups, including Enterococcus gallinarum, Lactobacillus acidipiscis, L. coryniformis subsp. coryniformis, L. coryniformis subsp. torquens, L. curvatus, L. paraplantarum, L. plantarum subsp. argentoratensis, L. plantarum subsp. plantarum, L. sakei subsp. carnosus, Lactococcus garvieae, Lactococcus lactis subsp. cremoris, Leuconostoc pseudomesenteroides, Pediococcus acidilactici, Pediococcus pentosaceus, Weissella hellenica, Weissella paramesenteroides and Carnobacterium divergens. This is the first report to document that C. divergens, L. acidipiscis, L. sakei subsp. carnosus, L. garvieae, phenotypically novel L. lactis subsp. cremoris, E. gallinarum and W. hellenica are present in vegetative forage crops. L. plantarum group strains were most frequently isolated from the badly preserved silages. Some isolates showed a wide range of growth preferences for carbohydrate utilization, optimal growth pH and temperature in vitro, indicating that they have a high growth potential. These results are useful in understanding the diversity of LAB associated with decayed silage of timothy and orchardgrass.

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Production of 3-Hydroxypropionaldehyde in Silage Inoculated withLactobacillus coryniformisPlus Glycerol

Cited by 17 publications

References 11 publications

Genotypic and phenotypic characterization of lactic acid bacteria isolated from Italian ryegrass silage

Genotypic and phenotypic characterization of lactic acid bacteria isolated from Italian ryegrass silage

Methylotrophic yeast, lactic acid bacteria and glycerine as additives for sugarcane silage

Identification and characterization of lactic acid bacteria isolated from mixed pasture of timothy and orchardgrass, and its badly preserved silage

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