Production of 6-pentyl-α-pyrone by trichoderma harzianum in solid-state fermentation

Abstract

Many Trichoderma species are able to produce 6-pentyl-α-pyrone (6-PP), a lactone with coconut-like aroma. In the present work, several culture parameters were studied to enhance the production of 6-PP by Trichoderma harzianum 4040 in solid-state fermentation. Green coir powder added to a nutrient solution was used as support material for fermentation. A Plackett-Burman screening technique was applied, followed by a fractionary factorial design. The best culture conditions within the experimental domain studied were (100 g support)-1 : sucrose, 3 g; NaNO3, 0.24 g; (NH4)2SO4, 0.18 g; KH2PO4, 0.1 g; inoculum concentration, 2.2 x 10 6 spores; moisture level, 55%. The temperature established was 28ºC. The fermentation under the selected conditions led to a 6-PP production six times higher (5.0 mg/g dry matter) than the initial one (0.8 mg/g dry matter) after seven days of cultivation.

Solid-state fermentation of agro-industrial wastes to produce bioorganic fertilizer for the biocontrol of Fusarium wilt of cucumber in continuously cropped soil

“…The fact that a disadvantageous condition for SQR-T037 biomass production showed a high 6PAP yield in this experiment was in agreement with the findings of Rocha-Valadez et al (2006) and was helpful in the practical treatment of the agro-industrial wastes because the inoculum content and fermentation days were the most costly factors. Although the 6PAP yield did not reach the 3-5 g kg À1 dry weight of solidstate fermentation substrate reported by de Aráujo et al (2002) and Ramos et al (2008), the additional energy spent on autoclaving the fermentation substrate was saved in our experiment.…”

Antimicrobial properties of 6-pentyl-α-pyrone produced by endophytic strains of Trichoderma koningii and its effect on aflatoxin B1 production

“…Fungal endophytes from medicinal plants represent an untapped pool of secondary metabolites that are used in pharmaceutical, food and agricultural industries (Sharma et al 2016). Among the bioactive compounds produced by Trichoderma species, the lactone 6PP, a compound with a characteristic coconut aroma, is used in food, cosmetic and detergent industries (El-Hasan et al 2008;Ramos et al 2008;Oda et al 2009). Most previous reports were focused on production of 6PP by certain species of Trichoderma fungi occurring in soil (Collins & Halim 1972;Simon et al 1988;Benoni et al 1990;Worasatit et al 1994;El-Hasan et al 2007).…”

Mechanistic Approaches toward Rational Design of a Heterogeneous Catalyst for Ring-Opening and Deoxygenation of Biomass-Derived Cyclic Compounds

“…Similar to TAL, 6-amyl-α-pyrone (6PP) , is another 2-pyrone molecule which is derived directly from the solid state fermentation of biomass and was demonstrated as a potential platform molecule (Figure (d)) . Catalytic transformation routes for the valorization of 6PP were explored by Alam et al , 6PP was shown to undergo ring-opening and decarboxylation reaction at 498 K to produce a food grade flavoring agent, non-2-en-4-one (conversion = 95% and selectivity = 60%) .…”