Production of a bacteriocine-like substance by group-A streptococci of M-type 4 and T-pattern 4

Johnson, Diana W.; Tagg, John; Wannamaker, Lewis W.

doi:10.1099/00222615-12-4-413

Cited by 29 publications

(18 citation statements)

References 31 publications

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“…Recently, Farkas-Himsley and Page1 (1 977) have attempted to make bacteriocine typing more quantitative by mixing suspensions of test strains with standardised bacteriocine preparations and detecting the presence of leaked ultravioletlight-absorbing material. Similar principles might eventually be applicable to the inhibitor typing of streptococci, but so far it has been possible to make cell-free preparations only of the inhibitors produced by strains P1 (Johnson et al, 1979), P2 (Tagg et al, 1973b) and P3 (Tagg et al, 1975). Similar difficulties have been encountered in other investigations of inhibitor production by gram-positive bacteria .…”

Section: Discussionmentioning

confidence: 92%

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"Fingerprinting" -haemolytic streptococci by their production of and sensitivity to bacteriocine-like inhibitors

Tagg¹,

Bannister²

1979

Journal of Medical Microbiology

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152

163

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Section: Discussionmentioning

confidence: 92%

“…There were a few indications of a relationship between P-typing pattern and serological group, and two clear instances of a close correspondence between P-typing pattern and M serotype among group-A streptococci; one of these is described in detail elsewhere (Johnson et al, 1979). Others may be revealed by subsequent studies.…”

Section: Discussionmentioning

confidence: 93%

"Fingerprinting" -haemolytic streptococci by their production of and sensitivity to bacteriocine-like inhibitors

Tagg¹,

Bannister²

1979

Journal of Medical Microbiology

Self Cite

152

163

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“…Similarly, only the six M-type 57 strains tested were found to produce P-type 614 inhibitory activity (Tagg & Bannister, 1979). Proteinaceous antibiotics isolated from prototype strains representative ofthese two serotypes have subsequently been characterized as bacteriocins (Johnson, Tagg & Wannamaker, 1979;Simpson & Tagg, 1983).…”

Section: Introductionmentioning

confidence: 99%

A bacteriocin produced by certain M-type 49Streptococcus pyogenesstrains when incubated anaerobically

Tagg

Skjold

1984

J. Hyg.

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SummaryBacteriocin production (P)-typing of 75 M-type 49 group-A streptococci obtained from a variety of epidemiological incidents in different countries gave no evidence of production under the usual aerobic test conditions. However, with anaerobic incubation, 28% of the strains gave a pattern of inhibitory activity against the indicator strains which was indistinguishable from that previously attributed to the bacteriocin, streptococcin A-FF22 (SA-FF22). Isolation and partial purification of the M type 49 bacteriocin (SA-M49) by freeze–thaw elution from anaerobically grown lawn cultures, followed by ammonium sulphate precipitation and Sephadex chromatography, showed the activity to be associated with a heat-stable proteinaceous molecule of molecular weight approximately 8000 – properties similar to those of SA-FF22. SA-FF22 and SA-M49 were found to have identical inhibitory spectra including immunity of the producer strains to the inhibitory activity of both the homologous and heterologous bacteriocin preparations. SA-M49 production occurred in some strains of phage subtypes II, III and provisional VI and. since it was a consistent property for all isolates from single outbreaks of infection, it provides a means of discriminating between strains of each of these three phage subtypes. There was no evidence of any increased incidence of SA-M49 production in M-type 49 strains associated with nephritic sequelae

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“…The 63 salA-positive S. pyogenes strains differed, however, in that they harbored a variant (named salA1) of the SalA structural gene, encoding a SalA homologue (SalA1) that had conservative amino acid differences in residues 2 (R2K) and 7 (I7F) of the propeptide part of the molecule. Interestingly, expression of biologically active (inhibitory) levels of SalA1 by S. pyogenes was evident only in strains of serotype M4 (8,19).…”

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confidence: 99%

Production of the Lantibiotic Salivaricin A and Its Variants by Oral Streptococci and Use of a Specific Induction Assay To Detect Their Presence in Human Saliva

Wescombe¹,

Upton

Dierksen

et al. 2006

Appl Environ Microbiol

101

123

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Salivaricin A (SalA), the first Streptococcus salivarius lantibiotic to be characterized, appears to be inhibitory to most Streptococcus pyogenes strains. A variant of the SalA structural gene (salA1) is present in more than 90% of S. pyogenes strains, but only strains of M serotype 4 and T pattern 4 produce the biologically active peptide. The present study identifies four additional variants (salA2 to salA5) of the SalA structural gene and demonstrates that each of the corresponding inhibitory peptides (SalA2 to SalA5) is produced in vitro. These variants appear to be similar to SalA and SalA1 in their inhibitory activity against Micrococcus luteus and in their ability to act as inducers of SalA production. It had previously been shown that S. pyogenes strain SF370 had a deletion (of approximately 2.5 kb) in the salM and salT genes of the salA1 locus. In the present study, several additional characteristic deletions within the salA1 loci were identified. S. pyogenes strains of the same M serotype all share the same salA1 locus structure. Since S. salivarius is a predominant member of the normal oral flora of healthy humans, strains producing anti-S. pyogenes lantibiotics, such as SalA, may have excellent potential for use as oral probiotics. In the present study, we have used a highly specific SalA induction system to directly detect the presence of SalA in the saliva of humans who either naturally harbor populations of SalA-producing S. salivarius or who have been colonized with the SalA2-producing probiotic S. salivarius K12.

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Production of a bacteriocine-like substance by group-A streptococci of M-type 4 and T-pattern 4

Cited by 29 publications

References 31 publications

"Fingerprinting" -haemolytic streptococci by their production of and sensitivity to bacteriocine-like inhibitors

"Fingerprinting" -haemolytic streptococci by their production of and sensitivity to bacteriocine-like inhibitors

A bacteriocin produced by certain M-type 49Streptococcus pyogenesstrains when incubated anaerobically

Production of the Lantibiotic Salivaricin A and Its Variants by Oral Streptococci and Use of a Specific Induction Assay To Detect Their Presence in Human Saliva

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