Production of a bioflocculant from Aspergillus niger using palm oil mill effluent as carbon source

Aljuboori, Ahmad H. Rajab; Uemura, Yoshimitsu; Osman, Noridah; Yusup, Suzana

doi:10.1016/j.biortech.2014.08.038

Cited by 105 publications

(65 citation statements)

References 31 publications

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“…After 36 h of culture, the cells entered the death phase. The drop in flocculating activity and yield of RBBF-C9 during this period may be due to deflocculation enzyme activity that is caused by cell lysis [36, 59]. The pH significantly decreased from 10.4 to 9.3 in the first 24 h, which might have been caused by the production of acidic polysaccharides during cell growth [51, 56, 60].…”

Section: Resultsmentioning

confidence: 99%

“…Thus, bioflocculants have received increasing attention as nontoxic and biodegradable substitutes for conventional inorganic and chemically synthetic flocculants [24, 27] in various industrial fields such as wastewater treatment and microalgae harvest [28–34]. However, the commercial applications of bioflocculants are often limited by their high production cost due to the use of expensive substrates [35, 36]. To reduce production costs, activated sludges or various wastewaters such as potato starch wastewater and phenol-containing wastewater are used as inexpensive culture media for the production of bioflocculants [37–41].…”

Section: Introductionmentioning

confidence: 99%

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Valorization of untreated rice bran towards bioflocculant using a lignocellulose-degrading strain and its use in microalgal biomass harvest

Liu

Hao

Jiang

et al. 2017

Biotechnol Biofuels

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BackgroundMicroalgae are currently considered as a promising feedstock for the production of biofuels and high-value products. However, the efficient harvest of microalgal biomasses from their culture broth is a major challenge. The harvesting of algal biomass by flocculation combined with gravity sedimentation is more convenient and cost-effective than traditional methods such as centrifugation and filtration. Compared to inorganic and chemically synthetic flocculants, bioflocculants are a suitable choice for microalgal harvest due to their biodegradable and nontoxic properties. Nonetheless, the high production costs associated with expensive substrates hinder the commercial applications of bioflocculants. Previous studies have shown that the hydrolysates of lignocellulosic biomasses from dilute acid hydrolysis can be utilized as an inexpensive carbon source for the production of bioflocculants. However, the toxic by-products generated in the dilute acid hydrolysis step limit the efficiency of subsequent fermentation. The strains that produce bioflocculants by using untreated lignocellulosic materials can circumvent the pretreatment process, as well as promote the application of bioflocculants in microalgal harvest.ResultsUnder alkaline fermentation conditions, the alkaliphilic strain Bacillus agaradhaerens C9 secreted 1.69 IU/mL of alkali-tolerant xylanase and 0.06 IU/mL of cellulase, indicating that this particular strain can efficiently convert untreated rice bran into bioflocculant (RBBF-C9), thereby circumventing rice bran pretreatment for downstream fermentation. The optimal fermentation conditions that result in the highest bioflocculant yield (12.94 g/L) were as follows: 20 g/L of untreated rice bran, 3 g/L of yeast extract, and 20 g/L of Na2CO3 at 37 °C for 24 h. RBBF-C9 contained 74.12% polysaccharides and 4.51% proteins, and was estimated to be 137 kDa. Furthermore, the bioflocculant RBBF-C9 exhibited good flocculating efficiency (91.05%) of oil alga Chlorella minutissima UTEX2341 when 60 mg/L of RBBF-C9 was added into the algal culture broth.ConclusionsThis study demonstrated that untreated rice bran is a suitable inexpensive substrate for the production of bioflocculants, and thus provides a novel approach in utilizing rice bran. The extracted bioflocculants may be potentially used in biomass harvesting of the oil algae C. minutissima UTEX2341 from the culture broth.Electronic supplementary materialThe online version of this article (doi:10.1186/s13068-017-0780-6) contains supplementary material, which is available to authorized users.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Valorization of untreated rice bran towards bioflocculant using a lignocellulose-degrading strain and its use in microalgal biomass harvest

Liu

Hao

Jiang

et al. 2017

Biotechnol Biofuels

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“…For example, the production of bioflocculant by Aspergillus flavus was growth associated with the highest flocculating activity of 87.2% after 60 h at the early stationary phase (Aljuboori et al. ). A similar finding was observed with the bioflocculant MBF‐6 produced by Klebsiella pneumoniae YZ‐6 in which the production was parallel with the cell growth, and the highest flocculating activity of 91.5% was attained at the early stationary phase of growth at 60 h. Yang et al.…”

Section: Growth Phases During the Process Of Bioflocculant Productionmentioning

confidence: 99%

“…The bioflocculants produced by A. flavus and K. pneumoniae were cation independent, which showed an outstanding performance in kaolin clay suspension without the addition of metal ions (Aljuboori et al. ; Zhao et al. ).…”

Section: Factors Influencing the Flocculating Activity Of Bioflocculantmentioning

confidence: 99%

Implications for public health demands alternatives to inorganic and synthetic flocculants: bioflocculants as important candidates

et al. 2016

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Chemical flocculants are generally used in drinking water and wastewater treatment due to their efficacy and cost effectiveness. However, the question of their toxicity to human health and environmental pollution has been a major concern. In this article, we review the application of some chemical flocculants utilized in water treatment, and bioflocculants as a potential alternative to these chemical flocculants. To the best of our knowledge, there is no report in the literature that provides an up‐to‐date review of the relevant literature on both chemical flocculants and bioflocculants in one paper. As a result, this review paper comprehensively discussed the various chemical flocculants used in water treatment, including their advantages and disadvantages. It also gave insights into bioflocculants production, challenges, various factors influencing their flocculating efficiency and their industrial applications, as well as future research directions including improvement of bioflocculants yields and flocculating activity, and production of cation‐independent bioflocculants. The molecular biology and synthesis of bioflocculants are also discussed.

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“…Therefore, filamentous fungi have attracted biotechnological attention to treat water and wastewater due to their turbidity removal ability [5,6]. Fungal coagulants are reported to be used for the recovery of suspended solids (SS) from water and wastewater.…”

Section: Introductionmentioning

confidence: 99%

Fungal Coagulant for Reduction of Water Turbidity

Al‐Mamun¹,

Jebun²,

Alam³

et al. 2016

3rd International Conference on Civil, Biological and Environmental Engineering (CBEE-2016) Feb. 4-5, 2016 Bali (Indonesia)

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Abstract-Turbidity from raw water is, usually, removed from by adding chemical coagulants. These chemicals are hazardous to human body and not much environmentally friendly. Therefore, this study was intended to discover microbial coagulant which would be a new replacement for the chemical coagulants. There are potential microorganisms, those can produce bioactive compounds, which can lead to different charges and surface properties. As such, the main goal of this project was to reduce turbidity of river water using natural coagulant. The discovered microbial coagulant showed promising results which can be considered as safe and environmentally friendly as compared to the conventional chemical coagulants. Thus far, a potential fungal strain is successfully identified which could reduce turbidity of water from 900 NTU to 54 NTU. It is expected that further optimization of the process parameters will help reduce the turbidity of the water. However, the full-scale application of the findings and the economic evaluation of the new coagulant is not determined yet.

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Production of a bioflocculant from Aspergillus niger using palm oil mill effluent as carbon source

Cited by 105 publications

References 31 publications

Valorization of untreated rice bran towards bioflocculant using a lignocellulose-degrading strain and its use in microalgal biomass harvest

Valorization of untreated rice bran towards bioflocculant using a lignocellulose-degrading strain and its use in microalgal biomass harvest

Implications for public health demands alternatives to inorganic and synthetic flocculants: bioflocculants as important candidates

Fungal Coagulant for Reduction of Water Turbidity

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