Production of a sensitive antibody against sirolimus for chemiluminescence immunoassay potential in its therapeutic drug monitoring

Abstract

A competitive chemiluminescence immunoassay is established and applied to the TDM of sirolimus.

A gold-based immunochromatographic strip for the detection of sirolimus in human whole blood

“…Derivatives of sirolimus were synthesized as reported previously with some modifications. 26,28,29 The derivative reaction produced a carboxymethyl oxime which created a reactive group and was beneficial for the coupling of sirolimus to a carrier protein. Briefly, 1 mg of sirolimus was dissolved in 0.6 mL of methanol : water : pyridine (4 : 1 : 1, v/v/v) solution, and then 0.7 mg CMO was added.…”

“…24,25 Recently a type of chemiluminescent immunoassay using a polyclonal antibody against sirolimus has been reported. 26 However, compared to monoclonal antibodies (mAbs), its stability was worse. Moreover, the antibody was reported to have a high cross-reactivity (CR) with everolimus.…”

Recent advances in analytical methods for the therapeutic drug monitoring of immunosuppressive drugs

“…Thus, the ratio of light emitted from luciferase and the synthetic fluorophore permits the detection and quantification of the analyte. Zhang et al developed a competitive chemiluminescence immunoassay (CLIA) based on a specific antibody against SIR produced by its well‐synthesized immunogen. The SIR molecule was modified with succinic anhydride (hapten 1) and carboxymethoxylamine (hapten 2).…”

“…In extreme cases, such overestimation may lead to a medication dosing change and serious medical consequences. The trend to reduce cross-reactivity and improve new immunoassays has emerged in the literature for ISD measurement.In particular, new antibodies capable of reducing cross-reactivity have been widely investigated [68][69][70]. Wei et al 68 developed a sandwich enzyme-linked immunosorbent assay (ELISA) with 2 novel TAC antibodies, which offered a reduction in cross-reactivity with 9 out of 11 of TAC's common metabolites and analogs.…”

Preparation of an anti-dexamethasone monoclonal antibody and its use in development of a colloidal gold immunoassay

“…A scheme for the production of DEX-HS conjugates by the succinic anhydride method (Zhang et al, 2016) is shown in Figure 1. The steps are briefly described as follows.…”