Production of acetone, butanol and ethanol from palm oil waste by Clostridium saccharoperbutylacetonicum N1-4

Mun, Ok-Ju; Ishizaki, Ayaaki; Yoshino, Sadazo; Furukawa, Koichi

doi:10.1007/bf00129394

Cited by 44 publications

(2 citation statements)

References 5 publications

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“…Butanol is the most attractive biofuel alternative to ethanol because it has numerous desirable properties, including lower vapor pressure, higher calorific value, less corrosive properties, and a non-hygroscopic nature (Dürre 2007). Butanol can be produced from renewable resources through acetone-butanol-ethanol (ABE) fermentation (Lee et al 1995) using Clostridia as a high-performance butanol-producing strain (Tashiro et al 2004). The metabolic pathway of typical ABE fermentation of butanol-producing Clostridia strain (Jones and Woods 1986) is summarized in Fig.…”

Section: Introductionmentioning

confidence: 99%

Nanocellulose from oil palm biomass to enhance microbial fermentation of butanol for bioenergy applications

Hastuti

Darmayanti

Hardiningtyas

et al. 2019

BioRes

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Nanocellulose made by 2,2,6,6-tetramethylpiperidine-1-oxyl (TEMPO)-catalyzed oxidation, described as TEMPO-oxidized cellulose nanofibers (TOCNs), has a high density of negative charges on its surface. Its use in microbial fermentation systems is expected to benefit microbial process stability. In particular, microbial stability is strongly required in acetone–butanol–ethanol (ABE) fermentation associated with the solvent-extraction process of butanol production. Here, TOCNs derived from oil palm empty fruit bunches pulp were added to extractive ABE fermentation media containing glucose as a main source, which can be potentially obtained from biomass by saccharification. Then, microbial fermentation was carried out using free or immobilized bacterial cells, to produce butanol from glucose. The presence of TOCNs induced higher total butanol production in broth by improving the growth environment of Clostridium saccharoperbutylacetonicum N1-4, which was used as the butanol-producing strain. Microscopic analysis revealed that the spider-web-like TOCN network helped to entrap bacterial cells in alginate beads, by ionic crosslinking of TOCNs and alginates via Ca2+ ions, to increase stability of bacterial cells in the composite gel beads. The addition of TOCNs to fermentation media had significant positive effects on the total butanol yield.

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Section: Introductionmentioning

confidence: 99%

Nanocellulose from oil palm biomass to enhance microbial fermentation of butanol for bioenergy applications

Hastuti

Darmayanti

Hardiningtyas

et al. 2019

BioRes

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“…4:1 vs. 2:1 (Biebl 1999). Several reports on ABE production employing this strain used only glucose or xylose as the carbon source Shinto et al 2008;Tashiro et al 2007) or a limited number of complex substrates such as maize and molasses fermentation media (Shaheen et al 2000), palm oil waste (Lee et al 1995) and excess sludge from a local sewage disposal facility (Kobayashi et al 2005). A previous study by Shinto et al (2008) using a kinetic modeling method with glucose or xylose as the substrate in the TYA media suggested that the strain N1-4 was substrate-dependent.…”

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confidence: 99%

Production of Acetone, Butanol and Ethanol as Bioenergy Source Materials by Clostridium saccharoperbutylacetonicum N1-4 (ATCC 13564) using Different Substrates

Ambarsari¹,

Sonomoto

2013

Microbiol Indones

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The cost of raw materials has been known to be the major limitation on the economic feasibility of acetone-butanol-ethanol (ABE) production. About 60-70% of the total ABE production cost is the cost for substrates (Madihah et al. 2001). Therefore, many researches have been conducted to find the most economical substrates for ABE production, for examples those who used simple sugars such as glucose, lactose, galactose, and xylose (Shinto et al.2008;Keis et al. 2001;Bahl et al. 1986), or moreIn the effort of developing bioenergy source materials, a laboratorium study was performed to investigate the effects of substrate types and concentrations on the production of acetone-butanol-ethanol (ABE) from various substrates by Clostridium saccharoperbutylacetonicum N1-4 (ATCC 13564) in defined TYA (Tryptone-Yeast extract-Acetate) media. The results demonstrated that the ABE ratios produced by the strain N1-4 were greatly influenced by the types of substrate and the concentrations being used. It was also shown that fermentation of disaccharides (cellobiose and dextrin) by the strain N1-4 could give relatively as high butanol and ABE yields as glucose fermentation after 24 h fermentation time. The strain N1-4 was also found to be able to ferment xylans from birchwood sources producing a relatively high ABE concentration, especially at a prolonged incubation time (after 48 h incubation period). A subsequent experiment using several different concentrations of glucose or cellobiose revealed that the higher the concentration of the substrate being used, then the higher the total ABE concentration or productivity could be obtained but not necessarily in the same increasing ratio. To the best of our knowledge, there was no other previous study about the effect of substrate type and concentration on the ABE ratio by C. saccharoperbutylacetonicum N1-4 (ATCC 13564) using various substrates in defined TYA media, specifically by using xylans and dextrin substrates.Key words: ABE ratio, acetone-butanol-ethanol fermentation, biomass substrate effect, Clostridium saccharoperbutylacetonicum N1-4Dalam rangka mendapatkan bahan baku bioenergi, sebuah studi laboratorium dilakukan untuk mengetahui pengaruh dari jenis dan konsentrasi substrat biomasa terhadap proses fermentasi langsung yang menghasilkan aseton, butanol, dan etanol oleh bakteri Clostridium saccharoperbutylacetonicum N1-4 (ATCC 13564) dalam media TYA (Tryptone-Yeast extract-Acetate) yang terdefinisi. Hasil penelitian menunjukkan bahwa rasio dari aceton:butanol:etanol (rasio ABE) ternyata sangat dipengaruhi oleh jenis substrat biomass dan konsentrasi yang dipergunakan dalam proses fermentasinya. Data menunjukkan bahwa dengan menggunakan substrat disakarida (yang diwakili oleh dextrin dan cellobiose), strain N1-4 mampu menghasilkan ABE dengan yield yang relatif sama tinggi dengan menggunakan glukosa setelah masa fermentasi 24 jam. Strain N1-4 ternyata juga mampu memproduksi ABE dengan konsentrasi yang cukup tinggi dengan menggunakan substrat xylan yang diekstraksi dari kayu, wa...

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Cellulase production from palm oil mill effluent in Malaysia: Economical and technical perspectives

Hii¹,

Yeap²,

Mashitah³

2011

Engineering in Life Sciences

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The demand for cellulases has increased tremendously over the last few decades. This is due to its numerous applications in industry and also because it can be used to hydrolyze cellulosic materials into sugars that can be fermented into bioethanol and bio‐based products. This does not only open up a big and significant market for cellulases, but also provides another source of biofuel and bioenergy in the future. Nevertheless, the cost of the existing substrates for cellulase fermentation is very high if required for large‐scale production. Sustainable supplies and an economically feasible biomass are needed to reduce the cost of cellulase production. Palm oil mill effluent (POME) is rich in carbohydrates, proteins, nitrogenous compounds, lipids, minerals, cellulose, hemicelluloses and lignin. It can be used naturally as a fermentation medium, either for cellulase or other value‐added product fermentation. In Malaysia, a large and continually increasing amount of POME is produced every year because of the high global demand for palm oil. Hence, the development of cellulase production from POME is reviewed, covering the POME production, cellulase production and the major challenges together with the future prospects of these processes.

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Production of acetone, butanol and ethanol from palm oil waste by Clostridium saccharoperbutylacetonicum N1-4

Cited by 44 publications

References 5 publications

Nanocellulose from oil palm biomass to enhance microbial fermentation of butanol for bioenergy applications

Nanocellulose from oil palm biomass to enhance microbial fermentation of butanol for bioenergy applications

Production of Acetone, Butanol and Ethanol as Bioenergy Source Materials by Clostridium saccharoperbutylacetonicum N1-4 (ATCC 13564) using Different Substrates

Cellulase production from palm oil mill effluent in Malaysia: Economical and technical perspectives

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