Production of active lysozyme films by matrix assisted pulsed laser evaporation at 355 nm

Purice, A.; Schou, Jørgen; Kingshott, Peter; Dinescu, M.

doi:10.1016/j.cplett.2006.12.078

Cited by 48 publications

(26 citation statements)

References 22 publications

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“…2 the film deposition was about 4250 ng/cm 2 (average thickness of $35 nm) corresponding to an equivalent of $11 monolayers of lysozyme. The deposition rate depends strictly linearly on the concentration of lysozyme in the water solution [16].…”

Section: Resultsmentioning

confidence: 99%

“…The accuracy of the measurements was about 1 Hz corresponding to 1.29 Â 10 16 amu/cm 2 [3,13,15,16]. Usually, a deposition on a QCM was carried out with 3600 pulses with a repetition rate of 2 Hz.…”

Section: Methodsmentioning

confidence: 99%

“…The film with the substrate was placed on a stainless steel plate, a drop of 0.5 ml of the matrix was poured on the film and finally the film was dried in vacuum (10 À6 mbar). The average spectra were recorded after 2000 shots of a 3-ns pulsed 337-nm nitrogen laser in the MALDI-TOF system [16]. In all cases positive ion spectra were acquired using a linear mass detector.…”

Section: Methodsmentioning

confidence: 99%

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Characterization of lysozyme films produced by matrix assisted pulsed laser evaporation (MAPLE)

Purice

Schou

Kingshott

et al. 2007

Applied Surface Science

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Characterization of lysozyme films produced by matrix assisted pulsed laser evaporation (MAPLE)

Purice

Schou

Kingshott

et al. 2007

Applied Surface Science

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“…MAPLE experiments using water matrix were also performed at 355 nm laser wavelength [10], where the absorption of ice is close to the local minimum for the UV-visible range. This can also confirm that successful MAPLE deposition can be realized without the dominant absorption of matrix material.…”

Section: Discussionmentioning

confidence: 99%

“…MAPLE has been used for deposition of organic and biomaterial thin layers such as carbohydrates, biopolymers, proteins, etc. [5][6][7][8][9][10].…”

Section: Introductionmentioning

confidence: 99%

Comparative study on Pulsed Laser Deposition and Matrix Assisted Pulsed Laser Evaporation of urease thin films

et al. 2009

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Tailoring immobilization of immunoglobulin by excimer laser for biosensor applications

Sima

Axente

Ristoscu

et al. 2010

J Biomedical Materials Res

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The sheltered transfer and immobilization of rabbit anti-human antiserum immunoglobulin G (IgG) by matrix-assisted pulsed laser evaporation (MAPLE) are reported. The iced targets submitted to laser irradiation consisted of 0.2-2 mg/mL IgG blended or not with lipid (L-α-phosphatidylcholine dipalmitoyl) dissolved in distilled water-based saline buffer. Thin IgG coatings were obtained at room temperature onto glass, fused silica, or silicon substrates. Ten thousand subsequent laser pulses of 0.33, 0.5, or 0.67 J/cm(2) fluence were applied for the synthesis of each sample. Morphology and composition of the thin films were studied by optical, scanning, and atomic force microscopy and Fourier transformed infrared spectrometry. Optical labeling methods such as spectrofluorimetry and fluorescence microscopy were selected to verify the biosensor transduction principle because of their high sensitivity for detecting low amounts of antigen (IgG). Protein immobilization to the substrate surface was demonstrated for all obtained structures after immersion in the donkey anti-rabbit secondary antibody solution. The IgG transfer and immobilization onto substrates were improved by addition of lipid to MAPLE solutions.

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Production of active lysozyme films by matrix assisted pulsed laser evaporation at 355 nm

Cited by 48 publications

References 22 publications

Characterization of lysozyme films produced by matrix assisted pulsed laser evaporation (MAPLE)

Characterization of lysozyme films produced by matrix assisted pulsed laser evaporation (MAPLE)

Comparative study on Pulsed Laser Deposition and Matrix Assisted Pulsed Laser Evaporation of urease thin films

Tailoring immobilization of immunoglobulin by excimer laser for biosensor applications

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