Production of active mutant strains of penicillium canescens 20171, forming complex preparation of exogenous glycosidases

Lomkatsi, E. T.; Radiani, T. Sh.; Shkolni, A. T.; Bilaĭ, T I; Gomarteli, M.; Jobava, M.; Tsereteli, A. K.; Kvesitadze, G.

doi:10.1002/abio.370100413

Acta Biotechnologica

1990

DOI: 10.1002/abio.370100413

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Production of active mutant strains of penicillium canescens 20171, forming complex preparation of exogenous glycosidases

E. T. Lomkatsi¹,

T. Sh. Radiani²,

A. T. Shkolni³

et al.

Abstract: The ability to synthesize extracellular 8-galactosidase (EC 3.2.1.23), a-galactosidase (EC 3.2.1.22), 8-fructofuranosidase (EC 3.2.1.26) in Penicillium canescens mutant strains (300 cultures) after UV-irradiation has been checked. Most of the investigated mutants had the ability to produce the mentioned enzymes in an increased amount in comparison with initial strain. The ability of extracellular enzyme biosynthesis in P. canescens mutant strains was increased for a-galactosidase on 200y0, for /l-galactosidase… Show more

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Enhancement of β‐galactosidase productivity of Aspergillus niger NCIM‐616

Rasouli

Ulkarni

1994

Journal of Applied Bacteriology

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A series of mutations was carried out with Aspergillus niger NCIM-616 as the parent strain. A mutant strain NG-4 with 28% increased beta-galactosidase productivity was produced with N-methyl-N-nitro-N-nitrosoguanidine (NTG) at 1500 micrograms ml-1 concentration and exposure time of 60 min. This mutant yielded a third generation u.v.-treated strain, UV-5 with a 117.6% increase in beta-galactosidase productivity with respect to the parent strain.

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Enhancement of β‐galactosidase productivity of Aspergillus niger NCIM‐616

Rasouli

Ulkarni

1994

Journal of Applied Bacteriology

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Glucose oxidase overproducing mutants ofPenicillium variabile(P16)

Petruccioli

Piccioni

Federici

et al. 1995

FEMS Microbiology Letters

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Conidia of Penicillium variabile P16 were subjected to mutagenesis and selection for glucose oxidase production on media containing o‐dianisidine. Studies of the relationship between dose of UV irradiation and conidial survival and frequency of mutation showed that the best frequency of positive mutation (17%) was obtained in correspondence to a conidial survival of 52%. Out of 54 overproducing mutants tested in shaken flasks, M‐80.10 showed the highest level of glucose oxidase activity (127% higher than the wild‐type). M‐80.10 mutant, transferred every 15 days to fresh medium and tested monthly for 8 months, appeared stable. The time course of growth and enzyme production by the mutant M‐80.10 showed an increase of the glucose oxidase activity in the culture medium up to 19 U ml−1 after 96 h of fermentation.

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Mutagenesis and Analysis of Mold Aspergillus niger for Extracellular Glucose Oxidase Production Using Sugarcane Molasses

Singh

2006

ABAB

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Aspergillus niger ORS-4.410, a mutant of A. niger ORS-4, was generated by repeated ultraviolet (UV) irradiation. Analysis of the UV treatment dose on wild-type (WT) A. niger ORS-4, conidial survival, and frequency of mutation showed that the maximum frequency of positive mutants (25.5%) was obtained with a 57% conidial survival rate after the second stage of UV irradiation. The level of glucose oxidase (GOX) production from mutant A. niger ORS-4.410 thus obtained was 149% higher than that for WT strain A. niger ORS-4 under liquid culture conditions using hexacyanoferrate (HCF)-treated sugarcane molasses (TM) as a cheaper carbohydrate source. When subcultured monthly for 24 mo, the mutant strain had consistent levels of GOX production (2.62 +/- 0.51 U/mL). Mutant A. niger ORS-4.410 was markedly different from the parent strain morphologically and was found to grow abundantly on sugarcane molasses. The mutant strain showed 3.43-fold increases in GOX levels (2.62 +/- 0.51 U/mL) using HCF-TM compared with the crude form of cane molasses (0.762 +/- 0.158 U/mL).

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Production of active mutant strains of penicillium canescens 20171, forming complex preparation of exogenous glycosidases

Cited by 3 publications

References 4 publications

Enhancement of β‐galactosidase productivity of Aspergillus niger NCIM‐616

Enhancement of β‐galactosidase productivity of Aspergillus niger NCIM‐616

Glucose oxidase overproducing mutants ofPenicillium variabile(P16)

Mutagenesis and Analysis of Mold Aspergillus niger for Extracellular Glucose Oxidase Production Using Sugarcane Molasses

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