Production of Alpha Toxin of Clostridium Perfringens Ii. Failure to Produce a Potent Alpha Toxin in the Simplified Media

Molecular genetics and pathogenesis of Clostridium perfringens.

“…2b), which is also recognized by E. coli RNA polymerase (176). There are reports that growth medium composition affects the production of a-toxin (138,139), suggesting the existence of a possible control network which responds to the availability of a given nutrient. Many bacterial virulence factor genes are controlled as part of global regulons (130), and some phospholipase C genes are induced in response to phosphate limitation (152).…”

Clostridium Perfringens Food Poisoning

“…Jayko & Lichstein (78) added glycyl-L-asparagine to the medium used by Boyd et al (11) and found slight amounts of toxin produced. Murata et al (116) were unable to obtain alpha toxin production in a semisynthetic medium. Later, Murata et al (118,190) reported that C. perfringens pro duced alpha toxin if large amounts of arginine were present in the medium (119).…”