Production of antibody against a synthetic peptide of Porphyromonas gingivalis 40-kDa outer membrane protein

Abstract

OriginalProduction of antibody against a synthetic peptide of Porphyromonas gingivalis 40-kDa outer membrane protein Abstract: Porphyromonas gingivalis has been implicated as a major pathogen in periodontal diseases. We previously cloned a 40-kDa outer membrane protein (OMP) gene from P. gingivalis 381 and succeeded in producing sufficient quantities of the recombinant protein for experimental use. Since antibodies against the recombinant (r) 40-kDa OMP have potent ability to kill P gingivalis cells by complement activation and opsonization, r40-kDa OMP has been the subject of considerable interest as a possible vaccine candidate. In this study, in order to develop a component vaccine, the immunodominant domain in 40-kDa OMP was identified. Peptides corresponding to portions of the Nterminal regions of 40-kDa OMP were synthesized chemically and their immunoreactivities with antibody against r40-kDa OMP were tested. The 16-mer peptide, LDDEYKERVFQTFVHY, was found to react strongly with the antibody. Furthermore, a rabbit antibody was prepared by immunization with the 16-mer peptide, cross-linked with dehydrofolate reductase, and its immunoreactivity was then examined. In a BIAcore experiment the antibody clearly reacted with r40-kDa OMP as well as P gingivalis strains. These findings suggest that the 16-mer synthetic peptide may be useful for development of a component vaccine against P. gingivalis. (J. Oral Sci. 45, 111-116, 2003)

Humoral immune response to antigens of Porphyromonas gingivalis ATCC 33277 in chronic periodontitis

“…The results of this investigation indicated that patients with chronic periodontitis showed high serum levels of IgG against the soluble extract of P. gingivalis ATCC33277 while patients with gingivitis and periodontally healthy individuals presented a weak immunoreactivity to this antigen. This finding is in accordance with the outcomes of several studies, which have demonstrated higher antibody titers in subjects with periodontitis compared to healthy controls 4 , 6 , 14 , 16 .…”

Respiratory syncytial virus detection in cells and clinical samples by using three new monoclonal antibodies

“…Dot‐blot assays, along with the other techniques described in this article, provide useful information about the sensitivity of each particular mAb for viral antigens detection. As reported previously [Bhunia et al, ; Tsurumi et al, ], the dot‐blot technique can be considered as a valid, useful and affordable method to qualitatively study the antigen–antibody binding reaction. These studies showed that these mAbs are specific for the detection of the respective RSV protein tested.…”

Production and characterization of murine monoclonal antibody against synthetic peptide of CD34

“…The use of synthetic peptide immunogens as means to generate specific immunological reagents for a variety of purposes has increased markedly in recent years [17].…”