1998
DOI: 10.1007/s11745-998-0264-0
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Production of antiserum for sensitive enzyme‐linked immunosorbent assay of 3‐carboxy‐4‐methyl‐5‐propyl‐2‐furanpropanoic acid by chemiluminescence

Abstract: To obtain a specific antiserum for use in enzyme-linked immunosorbent assay (ELISA) of 3-carboxy-4-methyl-5-propyl-2-furanpropanoic acid (CMPF), we prepared a hapten-carrier conjugate in which the CMPF hapten was linked to a carrier protein through the 5-(1-hydrazopropyl) group. The antisera raised against this antigen in guinea pigs had excellent specificity for CMPF, showing little cross-reactivity with closely related compounds and no significant cross-reactivities with other furan compounds. The results in… Show more

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Cited by 2 publications
(1 citation statement)
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“…Efforts to reduce 9 in serum by continuous ambulatory peritoneal analysis were partly successful (86). Several procedures were elaborated to determine 9 by HPLC methods (82,(90)(91)(92) or by immunoassay (93). By applying these sensitive methods, 9 can be determined even in hair and sweat (94).…”
Section: Catabolism Of F-acidsmentioning
confidence: 99%
“…Efforts to reduce 9 in serum by continuous ambulatory peritoneal analysis were partly successful (86). Several procedures were elaborated to determine 9 by HPLC methods (82,(90)(91)(92) or by immunoassay (93). By applying these sensitive methods, 9 can be determined even in hair and sweat (94).…”
Section: Catabolism Of F-acidsmentioning
confidence: 99%