Production of Biovanillin by One-Step Biotransformation Using Fungus Pycnoporous cinnabarinus

Abstract

The current study proposes a one-step biotransformation process for vanillin production from ferulic acid using the wild fungal strain Pycnoporous cinnabarinus belonging to the family Basidiomycete. Improvement of biotransformation conditions was performed in two steps; initially a one factor at a time method was used to investigate effects of medium composition variables (i.e., carbon, nitrogen) and environmental factors such as pH on vanillin production. Subsequently, concentrations of medium components were optimized using an orthogonal matrix method. After primary screening, glucose as carbon source and corn steep liquor and ammonium chloride as organic and inorganic nitrogen source, respectively, supported maximum biotransformation of ferulic acid to vanillin. Under statistically optimum conditions vanillin production from ferulic acid by P. cinnabarinus was 126 mg/L with a molar yield of 54%. The overall molar yield of vanillin production increased by 4 times.

Investigation of the Amycolatopsis sp. Strain ATCC 39116 Vanillin Dehydrogenase and Its Impact on the Biotechnical Production of Vanillin

“…The bioconversion of ferulic acid into vanillin was demonstrated previously for various microorganisms, including bacteria like Pseudomonas and Rhodococcus (5)(6)(7)(8) and fungi such as the basidiomycete Pycnoporus cinnabarinus (9). Additionally, several genes encoding the responsible enzymes in some of these organisms were heterologously expressed in Escherichia coli and enabled the corresponding strains to convert ferulic acid into vanillin (10,11).…”

Aromatic Metabolism of Filamentous Fungi in Relation to the Presence of Aromatic Compounds in Plant Biomass

“…In contrast, in other fungi the direct conversion of ferulic acid into vanillic acid and vanillin into vanillic acid have been described (Henderson & Farmer, 1955), as well as vanillic acid as an intermediate in the production of vanillin (Falconnier et al, 1994;Krings et al, 2001;Thibault et al, 1998;Tilay, Bule, & Annapure, 2010). Because this was usually based on the relative amounts of the two compounds and their conversion is reversible, it is difficult to say what the most common pathway is in fungi for vanillic acid and vanillin.…”

Method development for simultaneous detection of ferulic acid and vanillin using high-performance thin layer chromatography

“…The extracts and the residue were redissolved in 50% (v/v), respectively. The organic phase containing FA and vanillin was concentrated (up to 2 to 3 ml) using a rotary vacuum evaporator with conditions (55°C, 150 rpm, <80 mbar) followed by reconstitution in 2 ml of methanol 50% (v/v) (Tilay et al 2010). This solution was used for quantification of FA and vanillin by HPTLC.…”