Production of C20, C30 and C40 terpenes in the engineered phototrophic bacterium Rhodobacter capsulatus

Hage-Hülsmann, Jennifer; Klaus, Oliver; Linke, Karl; Troost, Katrin; Gora, Lukas; Hilgers, Fabienne; Wirtz, Astrid; Santiago‐Schübel, Beatrix; Loeschcke, Anita; Jaeger, Karl‐Erich; Drepper, Thomas

doi:10.1016/j.jbiotec.2021.07.002

Cited by 10 publications

(2 citation statements)

References 73 publications

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“…Sesterterpenes consist of relatively rare terpenoid substances that are formed by geranylfarnesyl diphosphate synthase (GFPPS) using GFPP as the precursor in the plastid MEP pathway [ 143 , 144 , 145 ]. Triterpenes are C 30 compounds derived from two molecules of FPP to generate the squalene catalyzed by squalene synthase (SQS) via the MVA pathway in the cytoplasm [ 26 , 146 , 147 ]. Although triterpenoids are not necessary for plant growth and development, the substances in this group have a wide range of biological activities and widespread commercial applications [ 148 , 149 , 150 , 151 ].…”

Section: Biosynthesis Pathways Of Terpenoidsmentioning

confidence: 99%

Biosynthesis and the Transcriptional Regulation of Terpenoids in Tea Plants (Camellia sinensis)

Wei

Yang

et al. 2023

IJMS

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Terpenes, especially volatile terpenes, are important components of tea aroma due to their unique scents. They are also widely used in the cosmetic and medical industries. In addition, terpene emission can be induced by herbivory, wounding, light, low temperature, and other stress conditions, leading to plant defense responses and plant–plant interactions. The transcriptional levels of important core genes (including HMGR, DXS, and TPS) involved in terpenoid biosynthesis are up- or downregulated by the MYB, MYC, NAC, ERF, WRKY, and bHLH transcription factors. These regulators can bind to corresponding cis-elements in the promoter regions of the corresponding genes, and some of them interact with other transcription factors to form a complex. Recently, several key terpene synthesis genes and important transcription factors involved in terpene biosynthesis have been isolated and functionally identified from tea plants. In this work, we focus on the research progress on the transcriptional regulation of terpenes in tea plants (Camellia sinensis) and thoroughly detail the biosynthesis of terpene compounds, the terpene biosynthesis-related genes, the transcription factors involved in terpene biosynthesis, and their importance. Furthermore, we review the potential strategies used in studying the specific transcriptional regulation functions of candidate transcription factors that have been discriminated to date.

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Section: Biosynthesis Pathways Of Terpenoidsmentioning

confidence: 99%

Biosynthesis and the Transcriptional Regulation of Terpenoids in Tea Plants (Camellia sinensis)

Wei

Yang

et al. 2023

IJMS

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“…We chose the early enzymatic step of the carotenoid pathway as suitable target, which is responsible for the conversion of the precursor molecule farnesyl pyrophosphate (FPP). FPP is a key metabolite that is essential for the synthesis of the photopigments spheroidene and spheroidenone (belonging to the class of tetraterpenoids) and heterologous sesqui-and triterpenoids (Figure 5A) (Troost et al, 2019;Hage-Hülsmann et al, 2021;Hilgers et al, 2021).…”

Section: Optimization Of Prholhi-2-eyfpmentioning

confidence: 99%

Light-mediated control of gene expression in the anoxygenic phototrophic bacterium Rhodobacter capsulatus using photocaged inducers

Hilgers

Hogenkamp²,

Klaus³

et al. 2022

Front. Bioeng. Biotechnol.

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Photocaged inducer molecules, especially photocaged isopropyl-β-d-1-thiogalactopyranoside (cIPTG), are well-established optochemical tools for light-regulated gene expression and have been intensively applied in Escherichia coli and other bacteria including Corynebacterium glutamicum, Pseudomonas putida or Bacillus subtilis. In this study, we aimed to implement a light-mediated on-switch for target gene expression in the facultative anoxygenic phototroph Rhodobacter capsulatus by using different cIPTG variants under both phototrophic and non-phototrophic cultivation conditions. We could demonstrate that especially 6-nitropiperonyl-(NP)-cIPTG can be applied for light-mediated induction of target gene expression in this facultative phototrophic bacterium. Furthermore, we successfully applied the optochemical approach to induce the intrinsic carotenoid biosynthesis to showcase engineering of a cellular function. Photocaged IPTG thus represents a light-responsive tool, which offers various promising properties suitable for future applications in biology and biotechnology including automated multi-factorial control of cellular functions as well as optimization of production processes.

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Heterologous Production of Plant Terpenes in the Photosynthetic Bacterium Rhodobacter capsulatus

Loeschcke

Hage-Hülsmann

Troost

et al. 2022

Plant Synthetic Biology

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Production of C20, C30 and C40 terpenes in the engineered phototrophic bacterium Rhodobacter capsulatus

Cited by 10 publications

References 73 publications

Biosynthesis and the Transcriptional Regulation of Terpenoids in Tea Plants (Camellia sinensis)

Biosynthesis and the Transcriptional Regulation of Terpenoids in Tea Plants (Camellia sinensis)

Light-mediated control of gene expression in the anoxygenic phototrophic bacterium Rhodobacter capsulatus using photocaged inducers

Heterologous Production of Plant Terpenes in the Photosynthetic Bacterium Rhodobacter capsulatus

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