Production of cellulase‐free xylanase by the recombinant <i>Bacillus subtilis</i> and its applicability in paper pulp bleaching

Verma, Digvijay; Satyanarayana, T.

doi:10.1002/btpr.1826

Cited by 20 publications

(7 citation statements)

References 43 publications

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“…The regular procedure of papermaking requires high measure of chemicals, which has brought about risky profluent transfer issues (Verma and Satyanarayana 2013). The pulp and paper industry has been scanning for novel biotechnology methods utilized for the replacement for a portion of the chemicals utilized as a part of the paper making process.…”

Section: Application Of Xylanase In Pulp and Paper Biobleachingmentioning

confidence: 99%

“…It has been shown that enzyme pre-treatment improves the dissemination of sodium hydroxide in both hardwoods and softwoods, and enhances conventional pulping of wood chips and pulp uniformity (Woldesenbet et al 2012). In conventional papermaking process, manufactures use huge amount of chemicals, which have caused hazardous effluent disposal problems (Ayyachamy and Vatsala 2007; Verma and Satyanarayana 2013). …”

Section: Introductionmentioning

confidence: 99%

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Microbial xylanases and their industrial application in pulp and paper biobleaching: a review

et al. 2017

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Xylanases are hydrolytic enzymes which cleave the β-1, 4 backbone of the complex plant cell wall polysaccharide xylan. Xylan is the major hemicellulosic constituent found in soft and hard food. It is the next most abundant renewable polysaccharide after cellulose. Xylanases and associated debranching enzymes produced by a variety of microorganisms including bacteria, actinomycetes, yeast and fungi bring hydrolysis of hemicelluloses. Despite thorough knowledge of microbial xylanolytic systems, further studies are required to achieve a complete understanding of the mechanism of xylan degradation by xylanases produced by microorganisms and their promising use in pulp biobleaching. Cellulase-free xylanases are important in pulp biobleaching as alternatives to the use of toxic chlorinated compounds because of the environmental hazards and diseases caused by the release of the adsorbable organic halogens. In this review, we have focused on the studies of structural composition of xylan in plants, their classification, sources of xylanases, extremophilic xylanases, modes of fermentation for the production of xylanases, factors affecting xylanase production, statistical approaches such as Plackett Burman, Response Surface Methodology to enhance xylanase production, purification, characterization, molecular cloning and expression. Besides this, review has focused on the microbial enzyme complex involved in the complete breakdown of xylan and the studies on xylanase regulation and their potential industrial applications with special reference to pulp biobleaching, which is directly related to increasing pulp brightness and reduction in environmental pollution.

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Section: Application Of Xylanase In Pulp and Paper Biobleachingmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Microbial xylanases and their industrial application in pulp and paper biobleaching: a review

et al. 2017

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“…However, they have lower specific activity and stability (Chang et al, 2017). Alternatively, Gram positive bacteria such as Lactobacillus species and B. subtilis have been used for xylanase expression (Liu et al, 2005;Verma and Satyanarayana, 2013). These species are capable of N-glycosylation and higher protein production levels as compared with E. coli.…”

Section: Genetic Engineering Of Microorganisms For Maximization Of Xymentioning

confidence: 99%

Production and applications of xylanases – an overview

Malhotra

Chapadgaonkar

2018

bta

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Endo-1, 4-β-xylanase is an enzyme that depolymerises xylan, a major component of lignocellulose. The economic utilization of lignocellulosic biomass as a feedstock for the production of fuel and chemicals would represent a paradigm shift in industrial carbon utilization, allowing sustainable resources to replace the non-renewable energy resources like petroleum. However, lignocellulose is a highly recalcitrant material that is extremely difficult to depolymerize. Many microorganisms possess repertoires of enzymes which act synergistically to decompose various components of lignocellulosic biomass into simple sugars. Biomass-utilizing organisms are widely distributed within different species of archaea, bacteria, fungi, protists, plants, and animals. These organisms possess numerous lignocellulolytic enzymes that can hydrolyze cellulose, hemicelluloses, or lignin. The present paper gives a detailed account of strategies for production, purification and application of microbial xylanases. Conversion of lignocellulose into fermentable sugars and subsequent conversion into bioethanol has been discussed in the present paper.

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“…In this context, biobleaching and biopulping processes have been explored over the past years. It has been shown that the use of xylanases may be an economically profitable alternative to conventional use of high amount of chemicals, which cause hazardous effluent disposal problems [12][13][14][15].…”

Section: Introductionmentioning

confidence: 99%

In silico screening and experimental analysis of family GH11 xylanases for applications under conditions of alkaline pH and high temperature

Talens-Perales

Sánchez-Torres

Marín-Navarro

et al. 2020

Biotechnol Biofuels

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Background Xylanases are one of the most extensively used enzymes for biomass digestion. However, in many instances, their use is limited by poor performance under the conditions of pH and temperature required by the industry. Therefore, the search for xylanases able to function efficiently at alkaline pH and high temperature is an important objective for different processes that use lignocellulosic substrates, such as the production of paper pulp and biofuels. Results A comprehensive in silico analysis of family GH11 sequences from the CAZY database allowed their phylogenetic classification in a radial cladogram in which sequences of known or presumptive thermophilic and alkalophilic xylanases appeared in three clusters. Eight sequences from these clusters were selected for experimental analysis. The coding DNA was synthesized, cloned and the enzymes were produced in E. coli. Some of these showed high xylanolytic activity at pH values > 8.0 and temperature > 80 °C. The best enzymes corresponding to sequences from Dictyoglomus thermophilum (Xyn5) and Thermobifida fusca (Xyn8). The addition of a carbohydrate-binding module (CBM9) to Xyn5 increased 4 times its activity at 90 °C and pH > 9.0. The combination of Xyn5 and Xyn8 was proved to be efficient for the saccharification of alkali pretreated rice straw, yielding xylose and xylooligosaccharides. Conclusions This study provides a fruitful approach for the selection of enzymes with suitable properties from the information contained in extensive databases. We have characterized two xylanases able to hydrolyze xylan with high efficiency at pH > 8.0 and temperature > 80 °C.

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Production of cellulase‐free xylanase by the recombinant Bacillus subtilis and its applicability in paper pulp bleaching

Cited by 20 publications

References 43 publications

Microbial xylanases and their industrial application in pulp and paper biobleaching: a review

Microbial xylanases and their industrial application in pulp and paper biobleaching: a review

Production and applications of xylanases – an overview

In silico screening and experimental analysis of family GH11 xylanases for applications under conditions of alkaline pH and high temperature

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