Production of Cellulose from<scp>D</scp>-Mannitol by<i>Acetobacter xylinum</i>KU-1

Oikawa, Tadao; Ohtori, Toshiyuki; Ameyama, Minoru

doi:10.1271/bbb.59.331

Cited by 42 publications

(26 citation statements)

References 3 publications

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“…Yeast extract and polypeptone are the most commonly used nitrogen source in BC production as it provides convenient nitrogens and growth factors for Acetobacter strains [6][7][8]. However, the high cost of yeast extract and polypeptone has a negative impact on the economics of its use in industrial-scale processes.…”

Section: Effect Of Molasses and Csl On Bc Productionmentioning

confidence: 99%

“…Therefore, one of the key aspects in the fermentation process is the development of a cost-effective culture medium to obtain maximum product yield. Most of the studies on the BC production by Acetobacter strains have been carried out in media containing pure sugar as carbon source, such as glucose, sucrose, fructose, mannitol, and arabitol [6][7][8]. The majority of studies on BC production have also used media containing complex nitrogen and vitamin sources such as yeast extract and polypeptone [6][7][8].…”

Section: Introductionmentioning

confidence: 99%

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Production and Characterization of Cellulose by Acetobacter sp. V6 Using a Cost-Effective Molasses–Corn Steep Liquor Medium

Jung

Lee

Jeong

et al. 2009

Appl Biochem Biotechnol

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In order to reduce of the manufacturing cost of bacterial cellulose (BC), BC production by Acetobacter sp. V6 was investigated in shaking culture using molasses and corn steep liquor (CSL) as the sole carbon and nitrogen sources, respectively. The highest BC production was obtained with Ca3(PO4)2-treated molasses. Maximum BC yield (2.21+/-0.04 g/l) was obtained at 5% (w/v) total sugar in molasses. In improved medium containing molasses and CSL, BC production was observed in the medium after 1 day of incubation and increased rapidly thereafter with maximum yield (3.12+/-0.03 g/l) at 8 days. This value was approximately twofold higher than the yield in the complex medium. Physical properties of BC from the complex and molasses media were studied using Fourier-transform infrared (FT-IR) spectroscopy and X-ray diffractometer. By FT-IR, all the BC were found to be of cellulose type I, the same as typical native cellulose. The relative crystallinity of BC produced in the complex and molasses media were 83.02 and 67.27%, respectively. These results suggest that molasses and CSL can be useful low-cost substrates for BC production by Acetobacter sp. V6 without supplementation with expensive nitrogen complexes such as yeast extract and polypeptone, leading to the reduction in the production costs.

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Section: Effect Of Molasses and Csl On Bc Productionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Production and Characterization of Cellulose by Acetobacter sp. V6 Using a Cost-Effective Molasses–Corn Steep Liquor Medium

Jung

Lee

Jeong

et al. 2009

Appl Biochem Biotechnol

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“…The optimum pH for cellulose production is 5.0 (13,16), and 3 production is associated with and proportional to growth (17). Cellulose production has been demonstrated from glucose, sucrose, fructose, glycerol, mannitol, arabitol, and many other substrates (5,13,16,18). Excess glucose is oxidized to gluconate in wild-type A. xylinum, which lowers the pH and inhibits cellulose production (13).…”

Section: Introductionmentioning

confidence: 99%

Production of Bacterial Cellulose from Alternate Feedstocks

Thompson

Hamilton

2001

ABAB

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SUMMARYProduction of bacterial cellulose by Acetobacter xylinum ATCC 10821 and 23770 in static cultures was tested from unamended food process effluents. Effluents included low-and high-solids potato effluents (LS & HS), cheese whey permeate (CW), and sugar beet raffinate (CSB). Strain 23770 produced 10% less cellulose from glucose than did 10821, and diverted more glucose to gluconate. Unamended HS, CW, and CSB were unsuitable for cellulose production by either strain, while LS was unsuitable for production by 10821. However, 23770 produced 17% more cellulose from LS than from glucose, indicating unamended LS could serve as a feedstock for bacterial cellulose.

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“…3, glycerol seems to be the best carbon source as a highest cell density (OD 600 ∼ 4.6) was obtained after 7 days cultivation. It has been reported that A. xylinum not only could metabolize mannitol but also a threefold higher growth could be obtained when compared with using glucose as carbon source [35]. However, as shown in Fig.…”

Section: Growth Curves Of Daao + a Xylinummentioning

confidence: 86%