Production of cephamycin C by Streptomyces clavuligerus NT4 using solid-state fermentation

Abstract

Cephamycin C is an extracellular broad spectrum beta-lactam antibiotic produced by Streptomyces clavuligerus, S. cattleya and Nocardia lactamdurans. In the present study, different substrates for solid-state fermentation were screened for maximum cephamycin C production by S. clavuligerus NT4. The fermentation parameters such as substrate concentration, moisture content, potassium dihydrogen phosphate, inoculum size and ammonium oxalate were optimized by response surface methodology (RSM). The optimized conditions yielded 21.68 +/- 0.76 mg gds(-1) of cephamycin C as compared to 10.50 +/- 1.04 mg gds(-1) before optimization. Effect of various amino acids on cephamycin C production was further studied by using RSM, which resulted in increased yield of 27.41 +/- 0.65 mg gds(-1).

Avermectin Production by Solid State Fermentation-a Novel Approach

“…Reports on secondary metabolite production under SSF showed a significant effect of initial pH of moistening medium on antibiotic production [13,17,[19][20][21][22], but in this study pH of moistening medium did not show any effect in biomass and avermectin production (table 2). This may be attributed to the fact that sorghum has high buffering capacity which resists change in pH as well as other tested parameters.…”

“…Sorghum supported maximum growth and avermectin production as compared to other substrates. Substrate-dependent variations in production of metabolites were also reported in the literature [17][18][19][20][21][22].…”

Evaluation of different media for the production of cephalosporins by Streptomyces clavuligerus ATCC 27064

“…The cephalosporins and CA biosynthetic pathways are completely different from each other, and like most bioactive compounds, they are rigorously controlled by the intra-and extra-cellular factors (Santamarta et al 2005). The presence of certain nutrients in the culture medium, such as the sources of C, N, P, or sulfur S, and salts, as well as the substances of the biosynthetic route can promote the production of CA or CephC (Bussari et al 2008). Glycerol is preferably used as C source for the production of CA, whereas starch is more suitable for the production of CephC (Zhu et al 2007;Sánchez et al 2010).…”

“…The positive effects of adding ornithine or high concentrations of lysine in synthetic culture medium of S. clavuligerus for the synthesis of CA or CephC, respectively, have been emphasized (Chen et al 2003;Domingues et al 2009). Several studies have shown that sulfur atom from the inorganic sources is more efficiently incorporated into the CephC molecule than sulfur from methionine or cysteine (Bussari et al 2008). Nevertheless, the potential benefit of adding such compounds in the complex media with the aim of improving the antibiotic production has been suggested only in the technical publications, such as patents (EgelMitani et al 1999).…”

Comparative Studies on Cephalosporin C biosynthesis by Aspergillus and Acremonium Species

“…. The promising effect of lysine, valine, cystine and methionine on antibiotic production was also reported by Bussari et al, 2008 andKagliwal et al, 2009. Enzymatic and genetic studies on methionine have revealed that it induces four of the enzymes of CPC biosynthesis at the level of transcription.…”