Production of clavulanic acid and cephamycin C by Streptomyces clavuligerus in palm-oil medium

Abstract

Palm and palm-kernel oils and their olein and stearin fractions were suitable as the main carbon sources for growth and production of clavulanic acid by Streptomyces clavuligerus. However, oleic and lauric acids were not utilized for growth. A spontaneous mutant, which was selected for higher cephamycin C production, also produced more clavulanic acid with these oils in the medium.

Utilization of vegetable oil in the production of clavulanic acid by Streptomyces clavuligerusATCC 27064

“…An alternative to maintaining the level of glycerol for long periods is substituting lipids for glycerol, as suggested by Butterworth (1984). So far only two pieces of work in the literature deal with the use of lipids in the CA production process by S. clavuligerus (Lee & Ho 1996;Large et al 1998). According to Large et al (1998), the addition of oil is preferred on an energy basis, as typical oil contains around 2.4 times the energy of glucose.…”

“…Their results suggest production of approximately 80 mg CA l )1 , with a medium containing an unspecified lipid. Lee & Ho (1996), working with various carbon sources, including fatty acids and palm oil, obtained higher titres (ca. 5 mg l )1 CA) with palm oil.…”

An Alternative Methodology for Determination of Cephamycin C from Fermentation Broth

“…There are a couple of reports in the literature dealing with cephamycin C production by these bacteria. Batch operations using immobilized cells of S. clavuligerus [2], solid state fermentation [3,4], and search for alternative carbon and nitrogen sources have been reported [5,6]. Currently, cephamycin C concentrations are determined by laborious and time-consuming biological methods, which do not provide good results.…”

An Approach to Strain Improvement and Enhanced Production of Clavulanic Acid inStreptomyces clavuligerus

“…p6621, of CA and cephamycin C in S. clavuligerus, and of tylosin in Streptomyces fradiae. [8][9][10] However, although the addition of oils to a production medium has been suggested as an exceptional method to avoid carbon catabolite regulation, residual oil levels at the end of the fermentation process and a higher oxygen requirement for oil metabolism are major disadvantages of this method that still need to be resolved. 11) In particular, residual oil levels have been reported to lead to increased medium viscosity and to affect further downstream processes.…”

“…2) Although there have been many studies suggesting that oil is a preferred carbon source for increasing CA titers, few studies have systematically examined the kinds of fatty acids that are important to CA production and the cell growth of the producer microorganisms. 9,12,13) To date, obtaining high titers of CA through strain improvements of S. clavuligerus has primarily depended on random mutagenesis and genetic engineering of the synthesis pathway of CA. [14][15][16][17][18][19] In this study, we examined the effects of synthetic and vegetable oils on the cell growth and CA production of S. clavuligerus in a 7-liter jar fermentor.…”