Production of Clinical-Type Dextran

Wolff, I. A.; Mellies, R. L; Lohmar, R. L.; Hellman, N. N.; Rogovin, S. P.; Watson, P. R.; Sloan, Jewell W.; Hofreiter, B. T.; Fisher, B. E.; Rist, C. R.

doi:10.1021/ie50540a055

Cited by 7 publications

(1 citation statement)

References 6 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Dextran70 (70 × 10 3 g mol –1 ) is referenced in the List of Essential Medicines by the World Health Organization (19th WHO model List of Essential Medicines, 2015). The chemical processes behind the production of these clinical fractions require several steps and are, consequently, quite costly, time-consuming, and non-eco-friendly, , stimulating the search for alternative and greener processes. Dextransucrase and dextranase activities have for example been combined to develop a one-step synthetic process of low molar mass dextrans. − Reactions in the presence of sucrose and sugar acceptors such as glucose and maltose were also investigated to favor the synthesis of low molar mass dextrans, but these conditions never totally suppressed the formation of high molar mass polymers. − …”

Section: Introductionmentioning

confidence: 99%

Investigations on the Determinants Responsible for Low Molar Mass Dextran Formation by DSR-M Dextransucrase

et al. 2017

View full text Add to dashboard Cite

Certain enzymes of the GH70 family dextransucrases synthesize very high molar mass dextran polymers, whereas others produce a mixed population of very high and low molar mass products directly from sucrose substrate. Identifying the determinants dictating polymer elongation would allow the tight control of dextran size. To explore this central question, we focus on the recently discovered DSR-M enzyme from Leuconostoc citreum NRRL B-1299, which is the sole enzyme that naturally, exclusively, and very efficiently produces only low molar mass dextrans from sucrose. Extensive biochemical and structural characterization of a truncated form of DSR-M (DSR-MΔ2, displaying the same biochemical behavior as the parental enzyme) and X-ray structural analysis of complexes with sucrose and isomaltotetraose molecules together with accurate monitoring of the resulting polymer formation reveal that DSR-MΔ2 adopts a nonprocessive mechanism attributed to (i) a high propensity to recognize sucrose as a preferred acceptor at the initial stage of catalysis, (ii) an ability to elongate oligodextrans irrespective of their size, and (iii) the presence of a domain V showing a weak ability to bind to the growing dextran chains. In this study, we present the 3D structure with the largest defined domain V reported to date in the GH70 family and map sugar binding pockets on the basis of the structure of the complex obtained with isomaltotetraose. Altogether, these findings give insights into the interplay between the domain V and the catalytic site during polymerization. They open promising strategies for GH70 enzyme engineering aiming at modulating glucan size.

show abstract

Section: Introductionmentioning

confidence: 99%

Investigations on the Determinants Responsible for Low Molar Mass Dextran Formation by DSR-M Dextransucrase

et al. 2017

View full text Add to dashboard Cite

show abstract

Studies on dextran and dextran derivatives. II. Acid hydrolysis of native dextran

Antonini¹,

Bellelli²,

Bonacci³

et al. 1964

Biopolymers

View full text Add to dashboard Cite

synopsisThe kinetics of the depolymerization of native dextran in dilute hydrochloric acid a t different temperatures has been followed by measurements of M,, a,,, [ q ] , (Pp)''', and S. The data allow the correlation of the various parameters of the total hydrolyzate a t various degrees of depolymerization. At a first approximation the results conform to a random splitting of a statistical, branched polymer.

show abstract

Angewandte Mikrobiologie: Dextran-Herstellung und Anwendung

Behrens

Ringpfeil

1964

Bericht Über Das Jahr 1963

View full text Add to dashboard Cite

Production of Clinical-Type Dextran

Cited by 7 publications

References 6 publications

Investigations on the Determinants Responsible for Low Molar Mass Dextran Formation by DSR-M Dextransucrase

Investigations on the Determinants Responsible for Low Molar Mass Dextran Formation by DSR-M Dextransucrase

Studies on dextran and dextran derivatives. II. Acid hydrolysis of native dextran

Angewandte Mikrobiologie: Dextran-Herstellung und Anwendung

Contact Info

Product

Resources

About