Production of cloned transgenic cow expressing omega-3 fatty acids

Wu, Xia; Ouyang, Hongsheng; Biao, Duan; Pang, Daxin; Zhang, Li; Yuan, Ting; Xue, Lian; Ni, Daibang; Liu, Cheng; Dong, Shuai; Wei, Zhuying; Li, Lin; Yu, Ming; Sun, Qingyuan; Chen, Da‐Yuan; Lai, Liangxue; Dai, Yifan; Li, Guangpeng

doi:10.1007/s11248-011-9554-2

Cited by 91 publications

(65 citation statements)

References 25 publications

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“…Moreover, the DHA content in white adipose tissue increased by 19% (Ji et al, 2009). Wu et al (2012) generated an mFat-1 transgenic calf, and found that the n-3 PUFA content increased in all samples, whilst that of n-6 PUFAs simultaneously decreased. Interestingly, the ratios of n-6/n-3 PUFAs in tissues and milk significantly decreased (Wu et al, 2012).…”

Section: High Performance Gas Chromatography Analysismentioning

confidence: 96%

“…The hFat-1-neo+, hFat-1-neo-, and PFF cells were cultured with 60 μM arachidonic acid for 48 h. Cells were then trypsinized for 3 min using 0.05% trypsin, and lipids were extracted as previously reported (Wu et al, 2012). Briefly, the cells were homogenized in a mixture of methanol, chloroform, and water.…”

Section: High-performance Gas Chromatography Analysis Of Fatty Acids mentioning

confidence: 99%

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Decreased expression of humanized Fat-1 in porcine fetal fibroblasts following deletion of PGK-neomycin resistance

Han¹,

Liang²,

Yun³

et al. 2015

Genet. Mol. Res.

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ABSTRACT. The neomycin-resistance (neo r ) gene is widely used as a selectable marker in eukaryotic expression vectors; however, its expression often affects that of target genes. Cre recombinase recognizes LoxP sites, leading to site-specific recombination and deletion of DNA and RNA between two LoxP sites. In the present study, a humanized Fat-1 gene (hFat-1) was generated by DNA Works and used to construct a pC-PGK-neo r -hfat-1 expression vector, in which PGK-neo r was flanked by two loxP sites. The pC-PGKneo r -hfat-1 plasmids were transfected into porcine fetal fibroblasts using liposomes, and three transgenic cell lines were obtained by culturing with 400μg/mL G418 for 7 days. Next, these cell lines 11595 PGK-neo r deletion in hFat-1 transgenic porcine fetal fibroblasts ©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 14 (3): 11594-11604 (2015) were transfected with a Cre recombinase expression plasmid, which contains a puromycin resistance gene, in order to delete neo r , which was integrated into the genome. hFat-1-neo r negative cells were obtained following puromycin selection. Real-time quantitative polymerase chain reaction data indicated that neomycin-resistant cells had higher hFat-1 expression than neomycin-sensitive cells. High performance gas chromatography data suggested that the n-6/n-3 ratio was significantly lower in transfected cells than in wild-type cells. The n-6/n-3 ratio in Cre-treated hFat-1-transfected cells was higher than that in untreated cells, suggesting that deletion of PGK-neo r decreased hFat-1 expression.

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Section: High Performance Gas Chromatography Analysismentioning

confidence: 96%

Section: High-performance Gas Chromatography Analysis Of Fatty Acids mentioning

confidence: 99%

Decreased expression of humanized Fat-1 in porcine fetal fibroblasts following deletion of PGK-neomycin resistance

Han¹,

Liang²,

Yun³

et al. 2015

Genet. Mol. Res.

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“…Simultaneously, fat-1 gene expression substantially reduced the n-6/n-3 PUFA ratio in tissue and milk. As the authors concluded, their results demonstrated that the transgenic fat-1 cow was characterised by normal reproduction and gave milk with improved fatty-acid composition, which might become an economically efficient approach to meet the increasing human demand for n-3 PUFAs (Wu et al, 2012).…”

Section: Modifications Of the Composition Of Milk Lipidsmentioning

confidence: 97%

“…A cloned transgenic cow expressing a fat-1 transgene from C. elegans and encoding an n-3 fatty-acid desaturase was generated by Wu et al (2012). The transgenic cow was healthy, and after artificial insemination became pregnant and delivered a healthy calf naturally.…”

Section: Modifications Of the Composition Of Milk Lipidsmentioning

confidence: 99%

“…Dietary fat not only directly affects the composition of adipose tissue of animals, but also influences the expression of genes related to lipid synthesis (Bernard et al, 2008;Duran-Montge et al, 2009). However, this method is limited by the huge demand for marine products and risk of their contamination with heavy metals (Wu et al, 2012). In case of milk there is also a possibility of direct supplementation with n-3 PUFA.…”

Section: Introductionmentioning

confidence: 99%

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The use of genetic engineering techniques to improve the lipid composition in meat, milk and fish products: a review

Świątkiewicz

Arczewska-Włosek

et al. 2015

Animal

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The health-promoting properties of dietary long-chain n-3 polyunsaturated fatty acids (n-3 LCPUFAs) for humans are well-known. Products of animal-origin enriched with n-3 LCPUFAs can be a good example of functional food, that is food that besides traditionally understood nutritional value may have a beneficial influence on the metabolism and health of consumers, thus reducing the risk of various lifestyle diseases such as atherosclerosis and coronary artery disease. The traditional method of enriching meat, milk or eggs with n-3 LCPUFA is the manipulation of the composition of animal diets. Huge progress in the development of genetic engineering techniques, for example transgenesis, has enabled the generation of many kinds of genetically modified animals. In recent years, one of the aims of animal transgenesis has been the modification of the lipid composition of meat and milk in order to improve the dietetic value of animal-origin products. This article reviews and discusses the data in the literature concerning studies where techniques of genetic engineering were used to create animal-origin products modified to contain health-promoting lipids. These studies are still at the laboratory stage, but their results have demonstrated that the transgenesis of pigs, cows, goats and fishes can be used in the future as efficient methods of production of healthy animal-origin food of high dietetic value. However, due to high costs and a low level of public acceptance, the introduction of this technology to commercial animal production and markets seems to be a distant prospect.

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Animal Cell Technology

2021

Advanced Fermentation and Cell Technology

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Production of cloned transgenic cow expressing omega-3 fatty acids

Cited by 91 publications

References 25 publications

Decreased expression of humanized Fat-1 in porcine fetal fibroblasts following deletion of PGK-neomycin resistance

Decreased expression of humanized Fat-1 in porcine fetal fibroblasts following deletion of PGK-neomycin resistance

The use of genetic engineering techniques to improve the lipid composition in meat, milk and fish products: a review

Animal Cell Technology

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