Production of diacylglycerols through glycerolysis with SBA‐15 supported Thermomyces lanuginosus lipase as catalyst

Abstract

BACKGROUND: In this study, SBA-15 was functionalized by silane coupling reagents, then lipase from Thermomyces lanuginosus (TLL) was immobilized onto the parent and the organically modified SBA-15 for diacylglycerol (DAG) production through glycerolysis.RESULTS: Diacylglycerol content of 54.77 ± 0.63%, and triacylglycerol (TAG) conversion of 77.75 ± 1.24%, were obtained from the parent SBA-15 supported TLL-mediated glycerolysis reaction in a solvent-free system. However, poor performance was unexpectedly observed when co-solvents were introduced into the reaction system. After organic modification, the functionalized SBA-15 supported TLL samples all exhibited reasonable performance, producing DAG content over 40 wt% and TAG conversion over 70 wt%. Higher DAG content, up to 59.19 ± 1.10%, was observed from the phenyl group-modified SBA-15 supported TLL. The operational stability of the immobilized TLL samples in glycerolysis was also improved after organic functionalization. The phenyl group-modified SBA-15 supported TLL showed good reusability in the present glycerolysis reaction, and 95.21 ± 4.87% of the initial glycerolysis activity remained after five cycles of reuse.CONCLUSION: The organic modification of SBA-15 improved the catalytic performance of its supported TLL in glycerolysis, in terms of TAG conversion, DAG content, and reusability.

Effects of Solvents on the Glycerolysis Performance of the SBA-15 Supported Lipases

“…In our previous study, we found that the enzymatic activity of the SBA-15 supported Lecitase ® Ultra LU in glycerolysis reaction was impaired by tert-pentanol, and considerable content of DAG was obtained from solvent-free system 23 . Similar results were also observed from the SBA-15 supported Lipase from Thermomyces lanuginosus TLL 4 . Therefore, study on the enzymatic glycerolysis activity in solvent systems would shed light on the foundation of enzymatic design for practical application.…”

“…MAG are food grade emulsifiers, widely used in food and cosmetic industries; DAG, especially 1,3-DAG, are recognized as functional cooking oil, due to their ability to reduce postprandial serum triacylglycerols TAG levels and prevent obesity, without loss of taste and processing functions 2,3 . Compared with chemical glycerolysis, enzymatic glycerolysis was more attractive, since its mild reaction conditions and no or less byproducts formation 4 . Yet the substrates of glycerolysis reaction, namely TAG and glycerol, are immiscible at low temperatures.…”

“…Besides, the surface area was large and the pore size distribution was narrow. SBA-15 was therefore an ideal candidate for lipase immobilization 4 . In this study, SBA-15 supported lipases, including CALB, LU and RML, were used to catalyze glycerolysis.…”

Quorum-Sensing Regulation of Antimicrobial Resistance in Bacteria

“…The large-scale use of antibiotics in clinical treatment has led to the formation of corresponding multi-resistance mechanisms by microorganisms against the target of antibiotics [19,20]. The main mechanisms are passivation of antibiotics through chemical modification, efflux pump systemic elimination of antibiotics, and modification of drug-targeting genes (Figure 1).…”

Structure‐guided preparation of fuctional oil rich in 1,3‐diacylglycerols and linoleic acid from Camellia oil by combi‐lipase

“…The less expensive Lipozyme RM IM and Lipozyme TL IM with a relatively lower activity have been considered as substitutes for Novozyme 435. Subsequently, some enzymatic systems, such as the presence of organic solvents or a solvent-free environment, 14,[17][18][19] the enzymatic method [20][21][22] and the reactive mode of a packedbed reactor, 23 have been investigated in DAG synthesis to improve esterification activity. The combination lipases possess the advantage of a synergistic effect compared to the individual enzymes, and have been investigated in biodiesel (fatty acid alkyl esters) production of transesterification/esterification [24][25][26] and hydrolysis, 27 as well as the synthesis of functional oil.…”