Production of diphtheria toxin by selected isolates of Corynebacterium ulcerans and Corynebacterium pseudotuberculosis

Wong, Truman; Groman, Neal B.

doi:10.1128/iai.43.3.1114-1116.1984

Cited by 28 publications

(10 citation statements)

References 18 publications

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“…It has already been shown (7) that these probes hybridize with DNA fragments from a variety of DT-positive C. diphtheriae. It also has been shown that DT-positive C. lulcerans and C. pseudotuberculosis (28) produce proteins with the molecular weight of DT and the ADP-ribosylating activity characteristic of that toxin.…”

Section: Pseudotuberculosis the Inhibition Of Toxin Synthesis By Fe21mentioning

confidence: 99%

Corynebacterium ulcerans and Corynebacterium pseudotuberculosis responses to DNA probes derived from corynephage beta and Corynebacterium diphtheriae

Groman¹,

Schiller²,

Russell³

1984

Infect Immun

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Strains of Corynebacterium ulcerans and Corynebacterium pseudotuberculosis (Corynebacterium ovis) were examined for the production of diphtheria toxin. A majority of C. ulcerans strains (25 of 37) and 1 C. pseudotuberculosis strain (1 of 14) gave a positive Elek test for diphtheria toxin, and for all strains but 1, production of diphtheria toxin was inhibited at the same level of Fe2' as was the Corynebacterium diphtheriae control. All Elek-positive cultures as well as two Elek-negative isolates of C. ulcerans gave a positive signal when hybridized with a DNA probe unambiguous for the diphtheria toxin gene (tox) under conditions of high stringency. The majority of probe-positive C. ulcerans strains contained three or more DNA restriction fragments that hybridized with converting corynephage II, suggesting that in C. ulcerans as in C. diphtheriae there may be a relationship between toxinogeny and carriage of I-related phage. Selected strains of C. diphtheriae, C. ulcerans, and C. pseudotuberculosis were examined for DNA homology by a semiquantitative technique. There was very little homology between C. diphtheriae and members of the other two species. Strains of C. ulcerans and C. pseudotuberculosis, although more closely related, appeared to belong to distinct species as well.

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Section: Pseudotuberculosis the Inhibition Of Toxin Synthesis By Fe21mentioning

confidence: 99%

Corynebacterium ulcerans and Corynebacterium pseudotuberculosis responses to DNA probes derived from corynephage beta and Corynebacterium diphtheriae

Groman¹,

Schiller²,

Russell³

1984

Infect Immun

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“…The global epidemiology of human infections caused by C. ulcerans is not well understood [2]. Many strains of C. ulcerans produce the diphtheria toxin [3] and cause exudative pharyngitis in humans indistinguishable from diphtheria [4], with local pseudomembrane production and potential systemic cardiac or neurologic manifestations as life-threatening complications. Independently of diphtheria toxin production, some strains produce a dermonecrotic toxin [5].…”

Section: Introductionmentioning

confidence: 99%

Rare human skin infection with Corynebacterium ulcerans: transmission by a domestic cat

et al. 2012

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Corynebacterium ulcerans is mainly known for its ability to cause animal infections. Some strains of C. ulcerans produce diphtheria toxin, which can cause life-threatening cardiopathies and neuropathies in humans. Human cutaneous C. ulcerans infection is a very rare disease that mimics classical cutaneous diphtheria. We present a very rare case of a C. ulcerans skin infection caused by a non-diphtheria toxin-producing strain of C. ulcerans that resolved after 3 weeks of therapy with amoxicillin-clavulanate. A pet cat was the probable source of infection. The presence of C. ulcerans in the mouth of the cat was confirmed by 16S rRNA gene analysis and the API Coryne system. In cases of human infection with potentially toxigenic corynebacteria, it is important to determine the species and examine the isolate for diphtheria toxin production. If toxigenicity is present, diphtheria antitoxin should be administered immediately. Carriers and potential infectious sources of C. ulcerans include not only domestic livestock but also pet animals. For the primary prevention of disease caused by diphtheria toxin-producing corynebacteria, vaccination with diphtheria toxoid is recommended.

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“…Among the species belonging to the ‘ C. diphtheriae group’, C. ulcerans and C. pseudotuberculosis are potential carriers of the exotoxin phospholipase D and the diphtheria‐like toxin (DT) (Wong and Groman, 1984; Funke et al., 1997). However, the occurrence of DT positive C. pseudotuberculosis strains seems to be very rare, as up to now, in only one case has the isolation of two strains originating from Egyptian buffaloes been described (Maximescu et al., 1974).…”

Section: Introductionmentioning

confidence: 99%

Corynebacterium ulcerans from Diseased Wild Boars

Contzen

Sting

Blazey

et al. 2011

Zoonoses and Public Health

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Two Corynebacterium strains were isolated from lymph nodes of wild boars showing severe alterations caused by caseous lymphadenitis. The wild boars came from different districts in southern Germany; one was found dead, the other had been shot. The two Corynebacterium strains obtained were both positive for phospholipase D. Further analysis of biochemical profiles did not allow unambiguous differentiation between Corynebacterium ulcerans and Corynebacterium pseudotuberculosis. Fourier-transformed infrared spectroscopy as well as partial sequencing of the genes for 16S rRNA and RNA polymerase beta subunit (rpoB) clearly identified both strains as Corynebacterium ulcerans. The tox gene for diphtheria toxin (DT) could be detected in both porcine isolates by PCR. Partial DNA sequencing of this tox gene showed significant differences from sequences described for other Corynebacterium ulcerans strains and a higher degree of similarity to that of Corynebacterium diphtheria. Production of diphtheria toxin could not be detected. These results indicate that wild game could be a reservoir for zoonotic Corynebacterium ulcerans.

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Production of diphtheria toxin by selected isolates of Corynebacterium ulcerans and Corynebacterium pseudotuberculosis

Cited by 28 publications

References 18 publications

Corynebacterium ulcerans and Corynebacterium pseudotuberculosis responses to DNA probes derived from corynephage beta and Corynebacterium diphtheriae

Corynebacterium ulcerans and Corynebacterium pseudotuberculosis responses to DNA probes derived from corynephage beta and Corynebacterium diphtheriae

Rare human skin infection with Corynebacterium ulcerans: transmission by a domestic cat

Corynebacterium ulcerans from Diseased Wild Boars

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