PRODUCTION OF DOCOSAHEXAENOIC ACID (DHA) FROM Thraustochytrium sp. ATCC 26185 USING DIFFERENTS NITROGEN CONCENTRATIONS

Furlan, Valcenir Júnior Mendes; Paulo, Maria do Castelo; Maus, Victor; Ferreira, Joana; Batista, Irineu; Bandarra, Narcisa M.

doi:10.5380/cep.v32i1.36925

Cited by 2 publications

(2 citation statements)

References 22 publications

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“…3) are similar to those previously obtained by Zhu et al 19 and Furlan et al 20 using Schizochytrium limacinum OUC88 and Thraustochytrium sp. ATCC 26185, respectively.…”

Section: Discussionsupporting

confidence: 90%

“…Zhu et al 19 found C14:0 (3.8–9.6%), C15:0 (2.1–10.1%), C16:0 (32.6–43.3%), DPA ω6 (7.1–8.2%) and DHA (29.8–36.5%) as the main fatty acids. Furlan et al 20 found C14:0 (1.5–9%), C15:0 (21–35%), C16:0 (5–33%), DPA ω6 (7–9%) and DHA (20–31.5%) as the main fatty acids. These results are similar to our results: C14:0 (1.8–16%), C15:0 (5–16%), C16:0 (9–32.5%) and the polyunsaturated, including DPA ω6 (6.5–14%) and DHA (20–43%).…”

Section: Discussionmentioning

confidence: 99%

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Production of docosahexaenoic acid by Aurantiochytrium sp. ATCC PRA-276

Furlan

Maus

Batista

et al. 2017

Brazilian Journal of Microbiology

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The high costs and environmental concerns associated with using marine resources as sources of oils rich in polyunsaturated fatty acids have prompted searches for alternative sources of such oils. Some microorganisms, among them members of the genus Aurantiochytrium, can synthesize large amounts of these biocompounds. However, various parameters that affect the polyunsaturated fatty acids production of these organisms, such as the carbon and nitrogen sources supplied during their cultivation, require further elucidation. The objective of this investigation was to study the effect of different concentrations of carbon and total nitrogen on the production of polyunsaturated fatty acids, particularly docosahexaenoic acid, by Aurantiochytrium sp. ATCC PRA-276. We performed batch system experiments using an initial glucose concentration of 30 g/L and three different concentrations of total nitrogen, including 3.0, 0.44, and 0.22 g/L, and fed-batch system experiments in which 0.14 g/L of glucose and 0.0014 g/L of total nitrogen were supplied hourly. To assess the effects of these different treatments, we determined the biomass, glucose, total nitrogen and polyunsaturated fatty acids concentration. The maximum cell concentration (23.9 g/L) was obtained after 96 h of cultivation in the batch system using initial concentrations of 0.22 g/L total nitrogen and 30 g/L glucose. Under these conditions, we observed the highest level of polyunsaturated fatty acids production (3.6 g/L), with docosahexaenoic acid and docosapentaenoic acid ω6 concentrations reaching 2.54 and 0.80 g/L, respectively.

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“…3) are similar to those previously obtained by Zhu et al 19 and Furlan et al 20 using Schizochytrium limacinum OUC88 and Thraustochytrium sp. ATCC 26185, respectively.…”

Section: Discussionsupporting

confidence: 90%

Section: Discussionmentioning

confidence: 99%

Production of docosahexaenoic acid by Aurantiochytrium sp. ATCC PRA-276

Furlan

Maus

Batista

et al. 2017

Brazilian Journal of Microbiology

Self Cite

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Application of the Response Surface Methodology to Optimize the Fermentation Parameters for Enhanced Docosahexaenoic Acid (DHA) Production by Thraustochytrium sp. ATCC 26185

Ding

Zhu

et al. 2018

Molecules

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The aim of this study was to determine the cumulative effect of fermentation parameters and enhance the production of docosahexaenoic acid (DHA) by Thraustochytrium sp. ATCC 26185 using response surface methodology (RSM). Among the eight variables screened for effects of fermentation parameters on DHA production by Plackett-Burman design (PBD), the initial pH, inoculum volume, and fermentation volume were found to be most significant. The Box-Behnken design was applied to derive a statistical model for optimizing these three fermentation parameters for DHA production. The optimal parameters for maximum DHA production were initial pH: 6.89, inoculum volume: 4.16%, and fermentation volume: 140.47 mL, respectively. The maximum yield of DHA production was 1.68 g/L, which was in agreement with predicted values. An increase in DHA production was achieved by optimizing the initial pH, fermentation, and inoculum volume parameters. This optimization strategy led to a significant increase in the amount of DHA produced, from 1.16 g/L to 1.68 g/L. Thraustochytrium sp. ATCC 26185 is a promising resource for microbial DHA production due to the high-level yield of DHA that it produces, and the capacity for large-scale fermentation of this organism.

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PRODUCTION OF DOCOSAHEXAENOIC ACID (DHA) FROM Thraustochytrium sp. ATCC 26185 USING DIFFERENTS NITROGEN CONCENTRATIONS

Cited by 2 publications

References 22 publications

Production of docosahexaenoic acid by Aurantiochytrium sp. ATCC PRA-276

Production of docosahexaenoic acid by Aurantiochytrium sp. ATCC PRA-276

Application of the Response Surface Methodology to Optimize the Fermentation Parameters for Enhanced Docosahexaenoic Acid (DHA) Production by Thraustochytrium sp. ATCC 26185

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