Production of eosinophil chemoattractant activity by ovine gastrointestinal nematodes

Wildblood, L. A.; Kerr, Karen; Clark, Douglas; Cameron, Alisdair; Turner, Darryl; Jones, Douglas

doi:10.1016/j.vetimm.2005.03.010

Cited by 25 publications

(25 citation statements)

References 29 publications

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“…ES has been reported to inhibit acid secretion in vitro [191], as well as restrain the enterochromaffin-like cells, which may indirectly cause diminished acid secretion by parietal cells [187; 192]. Ovine gastrointestinal nematode chemotaxins recruit granulocytes in vitro [193], which could be another way of damaging parietal cells in vivo [163; 167].…”

mentioning

confidence: 99%

Haemonchus Contortus and Ovine Host: A Retrospective Review.

Ashram¹,

Nasr²,

Mehmood³

et al. 2017

IJAR

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Gastrointestinal (GI) parasitic infections are a world-wide problem for both small-and large-scale farmers. Infection by GI parasites in ruminants, including sheep and goat can result in harsh economic losses in a variety of ways: reproductive inefficiency, decreased work capacity, involuntary culling, diminished food intake, poor animal growth rates and lower weight gains, treatment and management costs, and mortality in heavily parasitized animals. Among the GI parasites that cause losses to the farming industry, the barber"s pole worm Haemonchus contortus is the predominant, blood-sucking, highly pathogenic, and economically important nematode that infects small ruminants. Here, we review the historical and recent literature on the ovine-parasite-environment interaction for H. contortus to bring avenues where advances in the understanding of these interactions is an indispensible to develop a cost effective control strategies as potential options for the haemonchosis control in sheep and the proper management of sheep in various production systems.

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mentioning

confidence: 99%

Haemonchus Contortus and Ovine Host: A Retrospective Review.

Ashram¹,

Nasr²,

Mehmood³

et al. 2017

IJAR

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“…Both ECF and NCF activities were demonstrated in ES products and homogenates of H. contortus and T. circumcincta larvae and adult worms in a modified checkerboard assay based on Zigmond and Hirsch (1973), using a microchemotaxis chamber similar to those used for other studies of helminth products (Tanaka and Torisu 1978;Klesius et al 1985;Horii et al 1988;Ishida and Yoshimura 1990;Falcone et al 2001;Wildblood et al 2005). Neutrophil chemotaxis was demonstrated in 28 of 35 experiments and eosinophil chemotaxis in 20 of 38 experiments.…”

Section: Discussionmentioning

confidence: 91%

Section: Introductionmentioning

confidence: 99%

“…An eosinophil chemotactic factor (ECF) is not secreted by the free-living nematode Caenorhabditis elegans (Wildblood et al 2005), but is present in numerous parasitic cestodes (Horii et al 1984a;Potter and Leid 1986;Niwa et al 1998), trematodes (Owashi andIshii 1982;Horii et al 1984b;1986a), Anopheles saliva (Owhashi et al 2008), ES products of Psoroptes ovis (Wildblood and Jones 2007) and nematodes. These include L3, L4 and adult Teladorsagia circumcincta and Haemonchus contortus (Wildblood et al 2005), L3 Ostertagia ostertagi (Klesius et al 1985(Klesius et al , 1986, L3 Anisakis (Tanaka and Torisu 1978), adult Ascaris (Tanaka et al 1979), adult Angiostrongylus cantonensis (Ishida and Yoshimura 1990), adult Dirofilaria immitis (Owhashi et al 1996), Metastrongylus apri (Sasaki and Katsuno 1983), larval Trichinella spiralis and adult Trichuris muris (Dixon et al 2006). Neutrophil chemotactic factors (NCF) have been identified in parasitic cestodes (Horii et al 1984a) and trematodes (Horii et al 1984b;Jefferies et al 1996;Owhashi et al 2005), as well as Tritrichomonas foetus (Owhashi et al 1994), Necator americanus (Bower et al 2008), Oesophagostomum aculeatum , adult Ascaris (Tanaka et al 1979), D. immitis (Horii et al 1986b) and Oesophagostomum volvulus (Rubio de Krömer et al 1998), Trichinella spp.…”

Section: Introductionmentioning

confidence: 99%

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Neutrophil and eosinophil chemotactic factors in the excretory/secretory products of sheep abomasal nematode parasites

2011

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Both eosinophil chemotactic factor (ECF) and neutrophil chemotactic factor (NCF) activities were demonstrated in excretory/secretory (ES) products and homogenates of Haemonchus contortus and Teladorsagia circumcincta larvae and adult worms in a modified checkerboard assay using a micro-chemotaxis chamber. Neutrophil chemotaxis was seen in 28 of 35 experiments and eosinophil chemotaxis in 20 of 38 experiments. Chemokinetic activity for neutrophils and eosinophils (accounting for 40-50% of total cell migration) was also apparent in only three parasite products for each cell type. Significant NCF activity was present in six of seven adult worm ES products (three of four from T. circumcincta and in all three from H. contortus) and ECF activity in four of five adult ES products, whereas fewer L3 incubates, particularly of T. circumcincta, contained chemotactic activity. All parasite homogenates, with one exception for ECF, were chemotactic for both neutrophils and eosinophils. The sequential use of cellulose ultrafiltration membranes of decreasing pore size did not identify precisely the molecular weight of the NCF and ECF but indicated that the active chemicals were greater than 10 kDa and probably greater than 30 kDa.

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“…Numerous studies have demonstrated that various parasites synthesize molecules with the capacity to attract leukocytes, and products triggering eosinophil chemotaxis have been isolated from parasitic nematodes [12,13] . Compared to the relatively uniform structure of mammalian chemokines, chemoattractants derived from parasites are a more biochemically diverse collection of molecules.…”

Section: Introductionmentioning

confidence: 99%

Eosinophils Utilize Multiple Chemokine Receptors for Chemotaxis to the Parasitic Nematode <i>Strongyloides stercoralis</i>

Stein

Redding

Lee³

et al. 2009

J Innate Immun

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Protective innate immunity to the nematode Strongyloides stercoralis requires eosinophils in the parasite killing process. Experiments were performed to determine if an extract of S. stercoralis would trigger eosinophil chemotaxis, and to then compare the chemotactic migration response, including second messenger signals and receptors, to those mechanisms triggered by host chemoattractants. Eosinophils undergo both chemotaxis and chemokinesis to soluble parasite extract in transwell plates. Pretreatment of eosinophils with pertussis toxin, a G protein-coupled receptor inhibitor, inhibited migration of the eosinophils to the parasite extract. Likewise, blocking PI3K, tyrosine kinase, p38 and p44/42 inhibited eosinophil chemotaxis to parasite extract. Furthermore, CCR3, CXCR4 or CXCR2 antagonists significantly inhibited eosinophil chemotaxis to the parasite extract. Molecular weight fractionation of parasite extract revealed that molecules attracting eosinophils were present in several fractions, with molecules greater than 30 kDa being the most potent. Treating the extract with proteinase K or chitinase significantly inhibited its ability to induce chemotaxis, thereby demonstrating that the chemoattractants were both protein and chitin. Therefore, chemoattractants derived from parasites and host species stimulate similar receptors and second messenger signals to induce eosinophil chemotaxis. Parasite extract stimulates multiple receptors on the eosinophil surface, which ensures a robust innate immune response to the parasite.

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Production of eosinophil chemoattractant activity by ovine gastrointestinal nematodes

Cited by 25 publications

References 29 publications

Haemonchus Contortus and Ovine Host: A Retrospective Review.

Haemonchus Contortus and Ovine Host: A Retrospective Review.

Neutrophil and eosinophil chemotactic factors in the excretory/secretory products of sheep abomasal nematode parasites

Eosinophils Utilize Multiple Chemokine Receptors for Chemotaxis to the Parasitic Nematode <i>Strongyloides stercoralis</i>

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