Production of exopolysaccharide by Botryococcus braunii CCALA 778 under laboratory simulated Mediterranean climate conditions

García-Cubero, Rafael; Cabanelas, Iago Teles Dominguez; Sijtsma, L.; Kleinegris, Dorinde M.M.; Barbosa, María J.

doi:10.1016/j.algal.2017.12.003

Cited by 30 publications

(13 citation statements)

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“…This parameter determines the cell growth rate because it influences directly nutrient and light availability; therefore, having a direct impact in the biochemical composition. A steady state is achieved once both rates are equal (D = μ) [34]. Our data suggested that C. vulgaris possesses a resilient growth, adapting to different dilution rates.…”

Section: Discussionmentioning

confidence: 64%

“…The incident irradiance on reactor's surface was also evaluated. We tested a range of light intensities that embraced the maximum irradiances that typically can be found outdoors at different locations and/ or environmental situations (from low intensities typically found in northern latitudes, 1000 mol PAR m −2 s −1 to extremely high light intensities found in more meridional locations, 3000 mol PAR m −2 s −1 ) [14,34,40]. However, as San Pedro et al showed [41], the effect of light might be considered as light availability (I av ) and not just as the light irradiance that impinges on the reactor's surface.…”

Section: Discussionmentioning

confidence: 99%

“…However, these models were obtained from cultures under 24 h of continuous light, conditions that are not realistic when compared with outdoor cultivations. Regarding light intensities and photo-period, the range of irradiances used in our research simulated light conditions that typically are found outdoors [34] and therefore is more applicable to large-scale cultivation. The model predicted that C. vulgaris shows a slightly better yields under similar light intensities than Scenedesmus vacuolatus, strain previously modelled for CO 2 abatement purposes by our group [14].…”

Section: Discussionmentioning

confidence: 99%

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How to combine CO2 abatement and starch production in Chlorella vulgaris

et al. 2018

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Microalgae production has gained attention in recent years as promising systems for CO 2 abatement as well as a source of proteins, pigments, vitamins, lipids, and carbohydrates. Particularly, starch can be used for bioethanol production in a well-established fermentative process. The aim of this work was to maximize and model biomass productivity and CO 2 assimilation in continuous cultures of Chlorella vulgaris. The following culture parameters were studied: dilution rate, pH, temperature, light intensity, and nitrogen supply. The proposed model (r 2 = 0.95) predicted a maximum biomass productivity of 0.7 g L −1 d −1 and CO 2 assimilation of 1.3 g L −1 d −1. The experimental data agreed with these predictions, resulting in a maximum biomass productivity of 0.67 g L −1 d −1 (resulting in a CO 2 assimilation of 1.23 g L −1 d −1). In addition, the starch content was determined, and the results were used as input into a second model, which aimed at predicting starch accumulation during CO 2 abatement processes (r 2 = 0.84). This second model predicted a daily and continuous production of biomass with a maximum starch content of 0.25 g g −1 d −1 (25% dcw), but under different culture conditions than those found for maximizing biomass productivity and CO 2 assimilation. The maximum starch content experimentally determined was 0.2 g g −1 d −1 (20% dcw). Thus, to implement a biological system for CO 2 abatement coupled to starch accumulation, it is necessary to find a compromise between these two processes. Hence, although yield in both processes would be reduced, a simultaneous process for CO 2 mitigation and starch production would be feasible.

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Section: Discussionmentioning

confidence: 64%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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How to combine CO2 abatement and starch production in Chlorella vulgaris

et al. 2018

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“…The function of B. braunii colony size changes and the relation with light intensity are unclear (Zhang and Kojima, 1998;Khatri et al, 2014;García-Cubero et al, 2018). However, previous studies were performed in the presence of high CO 2 concentration, which does not correspond to physiological conditions.…”

Section: Unique Hl Acclimation Strategies Of B Braunii: Shading and mentioning

confidence: 99%

Light Acclimation of the Colonial Green Alga Botryococcus braunii Strain Showa

et al. 2019

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In contrast to single cellular species, detailed information is lacking on the processes of photosynthetic acclimation for colonial algae, although these algae are important for biofuel production, ecosystem biodiversity, and wastewater treatment. To investigate differences between single cellular and colonial species, we studied the regulation of photosynthesis and photoprotection during photoacclimation for the colonial green alga Botryococcus braunii and made a comparison with the properties of the single cellular species Chlamydomonas reinhardtii. We show that B. braunii shares some highlight (HL) photoacclimation strategies with C. reinhardtii and other frequently studied green algae: decreased chlorophyll content, increased free carotenoid content, and increased nonphotochemical quenching (NPQ). Additionally, B. braunii has unique HL photoacclimation strategies, related to its colonial form: strong internal shading by an increase of the colony size and the accumulation of extracellular echinenone (a ketocarotenoid). HL colonies are larger and more spatially heterogenous than low-light colonies. Compared with surface cells, cells deeper inside the colony have increased pigmentation and larger photosystem II antenna size. The core of the largest of the HL colonies does not contain living cells. In contrast with C. reinhardtii, but similar to other biofilm-forming algae, NPQ capacity is substantial in low light. In HL, NPQ amplitude increases, but kinetics are unchanged. We discuss possible causes of the different acclimation responses of C. reinhardtii and B. braunii. Knowledge of the specific photoacclimation processes for this colonial green alga further extends the view of the diversity of photoacclimation strategies in photosynthetic organisms.

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“…The CPS remains strongly associated to the cell envelope through covalent bond and plays role in pathogenesis. Many microorganisms including bacteria (Sardari et al, 2017), archaea (Squillaci et al, 2016), cyanobacteria (Bemal and Anil, 2017), fungi (Sun et al, 2016), yeast (Han et al, 2018), and microalgae (García-Cubero et al, 2018) are known to produce EPSs. EPS provides protection against the predators, antimicrobial agents and assist microorganisms to endure extremes of temperature (Casillo et al, 2017), salinity (Bemal and Anil, 2017;Isfahani et al, 2018), and desiccation (Tamaru et al, 2005;Knowles and Castenholz, 2008).…”

Section: Introductionmentioning

confidence: 99%

A Glacier Bacterium Produces High Yield of Cryoprotective Exopolysaccharide

et al. 2020

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Pseudomonas sp. BGI-2 is a psychrotrophic bacterium isolated from the ice sample collected from Batura glacier, Pakistan. This strain produces highly viscous colonies on agar media supplemented with glucose. In this study, we have optimized growth and production of exopolysaccharide (EPS) by the cold-adapted Pseudomonas sp. BGI-2 using different nutritional and environmental conditions. Pseudomonas sp. BGI-2 is able to grow in a wide range of temperatures (4-35 • C), pH (5-11), and salt concentrations (1-5%). Carbon utilization for growth and EPS production was extensively studied and we found that glucose, galactose, mannose, mannitol, and glycerol are the preferable carbon sources. The strain is also able to use sugar waste molasses as a growth substrate, an alternative for the relatively expensive sugars for large scale EPS production. Maximum EPS production was observed at 15 • C, pH 6, NaCl (10 g L −1), glucose as carbon source (100 g L −1), yeast extract as nitrogen source (10 g L −1), and glucose/yeast extract ratio (10/1). Under optimized conditions, EPS production was 2.01 g L −1 , which is relatively high for a Pseudomonas species compared to previous studies using the same method for quantification. High-performance anion-exchange chromatography with pulsed amperometric detection (HPAEC-PAD) analysis of EPS revealed glucose, galactose, and glucosamine as the main sugar monomers. Membrane protection assay using human RBCs revealed significant reduction in cell lysis (∼50%) in the presence of EPS, suggesting its role in membrane protection. The EPS (5%) also conferred significant cryoprotection for a mesophilic Escherichia coli k12 which was comparable to glycerol (20%). Also, improvement in lipid peroxidation inhibition (in vitro) resulted when lipids from the E. coli was pretreated with EPS. Increased EPS production at low temperatures, freeze thaw tolerance of the EPS producing strain, and increased survivability of E. coli in the presence of EPS as cryoprotective agent supports the hypothesis that EPS production is a strategy for survival in extremely cold environments such as the glacier ice.

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Production of exopolysaccharide by Botryococcus braunii CCALA 778 under laboratory simulated Mediterranean climate conditions

Cited by 30 publications

References 50 publications

How to combine CO2 abatement and starch production in Chlorella vulgaris

How to combine CO2 abatement and starch production in Chlorella vulgaris

Light Acclimation of the Colonial Green Alga Botryococcus braunii Strain Showa

A Glacier Bacterium Produces High Yield of Cryoprotective Exopolysaccharide

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