Production of extracellular enzymes in the entomopathogenic fungus Verticillium lecanii

Hasan, Sadid A.; Ahmad, Anis; Purwar, Abhinav; Khan, Nausheen; Kundan, Rishi; Gupta, Garima

doi:10.6026/97320630009238

Cited by 39 publications

(22 citation statements)

References 14 publications

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“…Proteinases were secreted by fungi growing in the media with both the maximal and minimal nitrogen content (Table 1), which suggests that their synthesis was constitutive. Pronounced activity of proteolytic enzymes in ento mopathogenic and carnivorous micromycetes may be associated with their involvement in the pathogenic processes in invertebrates [20,21]. It should be noted that zygomycetes were less active towards the protein substrates utilized than asco mycetes.…”

Section: Resultsmentioning

confidence: 99%

Screening of producers of proteinases with fibrinolytic and collagenolytic activities among micromycetes

et al. 2015

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Screening for producers of proteinases with fibrinolytic (plasmin like and plasminogen activa ting) and collagenolytic activities was carried out among 83 strains of microscopic fungi belonging to various ecological groups. Entomopathogenic micromycetes secreted proteinases with higher fibrinolytic and col lagenolytic activity than saprotrophic, potentially phytopathogenic, and epiphytic strains. Micromycete strains possessing proteolytic enzymes with collagenase activity were revealed, as were the strains producing proteinases with plasmin like activity. None of the strains studied secreted proteinases possessing only plas minogen activating activity. Tolypocladium inflatum k1 was found to be a producer of extracellular proteinases with high plasminogen activating, plasmin like, and collagenolytic activities. The specific plasminogen activating activity of T. inflatum k1 was shown to be 20% higher than its plasmin like activity.Specific values of plasminogen activating (1) and plasmin like (2) fibrinolytic activity of T. inflatum k1.

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Section: Resultsmentioning

confidence: 99%

Screening of producers of proteinases with fibrinolytic and collagenolytic activities among micromycetes

et al. 2015

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“…In Figure 6, V. lecanii colonization in the abdomen and the insect's body appear as a mass of mycelium with a few spores with cleavage of the cuticle and the presence of the fungal growth inside the body. The strain of V. lecanii was screened in a study (Hasan et al, 2013) and proved to be an efficient producer of protein and polysaccharide degrading enzymes (amylase, protease, and lipase). It was found to be an outstanding source of enzyme secretion and can be rationally utilized towards the production of improved mycopesticides.…”

Section: Resultsmentioning

confidence: 99%

The electron microscopic examination of fungal distortions in the adult red flour beetle, Tribolium castaneum H. (Coleoptera: Tenebrionidae)

Hanan¹

2017

J. Entomol. Nematol.

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The red flour beetle (Tribolium castaneum) is one of the main pests infecting cereals and causes damage to stored grains. Many pests, including beetles, are susceptible to infection by naturally occurring insect-pathogenic fungi (entomopathogenic fungi). In the present study, wheat flour collected from the local markets in the Jazan region of Saudi Arabia and dead bodies of T. castaneum adults were separated. The fungi associated with these insect dead bodies were identified. The result concluded that the most dominant fungi were Beauveria bassiana (61.67%) and Verticillium lecanii (38.33%). Fungi showed different stages of infection, such as adhesion, spore germination and mycelium colonization in the insect cadavers, as illustrated by scanning electron microscopy (SEM). The deformations, mycelium extortion and colonization, decomposition and erosion of the cuticle occurring in the different parts of the insects' cadavers were recorded by SEM. The results showed the presence of entomopathogenic fungi B. bassiana or V. lecanii on T. castaneum, as well as the susceptibility of T. castaneum adults to these fungi.

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“…However, Ali et al (2011) reported that the optimum extracellular protease activity of M. anisopliae was at pH 8. On the other hand, Hasan et al (2013) revealed that the highest proteolytic activity of the Verticillium lecanii was at pH 7-9.…”

Section: Effect Of Phmentioning

confidence: 96%

Assessment of the virulence and proteolytic activity of three native entomopathogenic fungi against the larvae of Oryctes agamemnon (Burmeister) (Coleoptera: Scarabaeidae)

Saleem

Ibrahim

2019

Egypt J Biol Pest Control

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Susceptibility of the third larval instar of the rhinoceros beetle, Oryctes agamemnon (Burmeister) (Coleoptera: Scarabaeidae), to the infection with three native entomopathogenic fungi (EPFs), Beauveria bassiana, Metarhizium anisopliae, and Lecanicillium lecanii, was determined under laboratory conditions. Three concentrations (10 4 , 10 6 , and 10 8 conidia/ml) of each EPF isolate were tested. Respective mortality rates of the larvae reached 34.2, 48.1, and 55.5% for B. bassiana, 39.5, 57.4, and 60.8% for M. anisopliae, and 17.4, 26.4, and 29.2% for L. lecanii. Mycosis test was performed to confirm the fungal infection of dead larvae. The LT 50 values for O. agamemnon larvae were 9.9 and 11.3 days, for the concentration which achieved 50% mortality for B. bassiana and M. anisopliae, respectively. The proteolytic activity of the three EPFs was investigated under different factors (incubation periods, temperature, and pH of fungal cultures). The results showed that the maximum activity of protease, produced by the three EPFs, was recorded after 6 days of incubation periods at 28°C, in a medium initially adjusted to pH 6.

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Production of extracellular enzymes in the entomopathogenic fungus Verticillium lecanii

Cited by 39 publications

References 14 publications

Screening of producers of proteinases with fibrinolytic and collagenolytic activities among micromycetes

Screening of producers of proteinases with fibrinolytic and collagenolytic activities among micromycetes

The electron microscopic examination of fungal distortions in the adult red flour beetle, Tribolium castaneum H. (Coleoptera: Tenebrionidae)

Assessment of the virulence and proteolytic activity of three native entomopathogenic fungi against the larvae of Oryctes agamemnon (Burmeister) (Coleoptera: Scarabaeidae)

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