Production of feather protein hydrolysate by keratinolytic bacterium Vibrio sp. kr2

Grazziotin, Adriane; Pimentel, Fernanda A.; Sangali, Sidnei; Jong, Erna Vogt de; Brandelli, Adriano

doi:10.1016/j.biortech.2006.10.034

Cited by 63 publications

(42 citation statements)

References 14 publications

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“…The time required for wool decomposition was however not shortened (data not shown). Our results are in good agreement with previous research [40,41].…”

Section: Bacillus Pumilus Strain Cicchlj Q89supporting

confidence: 94%

“…Interestingly, this strain of bacterium appears to develop considerable amounts of soluble proteins in comparison with other feather-degrading microorganisms. For instance, Bacillus subtilis DB100 (p5.2) released a maximum of 0.2 mg/ml soluble proteins after four days of incubation on 10 g/l feather [39], Streptomyces fradiae yielded a maximum of 0.12 mg/ml soluble proteins after 5 days [40,42], while Trichoderma atroviride strain F6 and a Streptomyces strain produced somewhat more, maximum values reaching 0.323 and 0.423 mg/ml after 8 and 3 days, respectively, when cultivated in 10 g/l feathers [43,44]. Growing a recombinant strain of keratinase-producing Bacillus subtilis on sheep wool-based distilled water medium yielded however as much as 1 mg/ml soluble protein after 3 days [45].…”

Section: Bacillus Pumilus Strain Cicchlj Q89mentioning

confidence: 99%

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A Bacillus Strain Able to Hydrolyze Alpha- and Beta-Keratin

Fellahi

Zaghloul²,

Feuk-Lagerstedt³

et al. 2014

J Bioproces Biotech

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The ability to hydrolyze keratin, a rigid and strongly cross-linked protein in the waste of poultry feather and sheep wool, has made keratinase production by microorganisms highly important to the biotechnological industry. A proteindegrading bacterium (C 4 ) was isolated from compost. Based on morphology and biochemical tests, along with 16S rRNA sequencing, the isolated C 4 was tentatively identified as Bacillus sp. C 4 (2008). The proteolytic activity of the Bacillus sp. C 4 strain was broadly specific; it degraded keratinous and non-keratinous proteins to different degrees. Pea pods as substrate generated the highest protease production, followed by soybean meal and sheep wool. Notwithstanding, using wool keratin as a sole source of carbon and nitrogen yielded the highest level of soluble proteins. Furthermore, the C 4 bacterium grew well, and produced a significant level of keratinase when using wool and feather as substrates. Supplementing the medium with yeast extract and peptone shortened the time required for feather degradation, but delayed the onset of the wool keratin hydrolysis with two days. The predominant amino acids released in feather hydrolysate were tyrosine, phenylalanine, and histidine. In contrast, the wool lysate was rich in aspartic acid, methionine, tyrosine, phenylalanine, histidine, and lysine. Results established that utilizing the C 4 strain for keratin degradation in waste management holds considerable potential.

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“…The time required for wool decomposition was however not shortened (data not shown). Our results are in good agreement with previous research [40,41].…”

Section: Bacillus Pumilus Strain Cicchlj Q89supporting

confidence: 94%

Section: Bacillus Pumilus Strain Cicchlj Q89mentioning

confidence: 99%

A Bacillus Strain Able to Hydrolyze Alpha- and Beta-Keratin

Fellahi

Zaghloul²,

Feuk-Lagerstedt³

et al. 2014

J Bioproces Biotech

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“…On the other hand, our finding was in agreement with others stated that, by increasing feather percent from 1-10% (w/v) in the growth media of two keratinolytic bacteria Vibrio sp. Kr2 and mutant strain of a B.subtilis, the percent of feather hydrolysis was increased (Grazziotin et al 2007;Cai et al 2008). Concerning the insignificant effect of inoculum size, our finding is in partial agreement with that of other report stated that by increasing the inoculum ratio from 2-10% there was no increase in the percent of feather hydrolysis (Cai et al 2008 On the other hand, the levels of NH 2 -free amino groups and soluble proteins verified in three optimized sheep wool -based media were 290.9-302.0 lmol leucine/ml and 3.4-4.6 mg/ml, respectively when these two combination sets [8.3% (w/v) sheep wool, 5.9% (v/v) inoculum size, 63.46 h] and [8.2% (w/v) sheep wool, 5.52% (v/v) inoculum size, 46.58 h] were applied, respectively.…”

Section: Attaining Optimized Conditionsmentioning

confidence: 97%

“…Isolation and purification of several keratinolytic enzymes from keratinolytic microorganisms including bacteria, actinomyctes and a lesser from fungi (Williams et al 1990;Böckle et al 1995;Santos et al 1996;Sangali and Brandelli 2000;Kim et al 2001;Nam et al 2002;Bernal et al 2006;Ionata et al 2008;Syed et al 2009;Khardenavis et al 2009;Cao et al 2009;Prakash et al 2010;Xie et al 2010) have been reported. Moreover, the use of keratinolytic enzymes was reported to be an excellent solution for recycling of these keratinous wastes (Onifade et al 1998;Grazziotin et al 2006;Grazziotin et al 2007;Deivasigamani and Alagappan 2008;Khardenavis et al 2009). …”

Section: Introductionmentioning

confidence: 96%

Optimizing the biodegradation of two keratinous wastes through a Bacillus subtilis recombinant strain using a response surface methodology

2010

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Statistical optimization of the biodegradation of two keratinous wastes directed by Bacillus subtilis recombinant cells was carried out by means of a response surface methodology. A Box-Behnken design was employed to predict the optimal levels of three variables namely, keratin percent, incubation time and inoculum size. Analysis of variance revealed that, only keratin percent had the highest significant effect. Canonical analysis and ridge max analysis were used to get the optimal levels of the three predictors along with the optimum levels of the responses. The optimal sets of predicted and validated levels of the three variables were [7.69% (w/v) feathers, 96.58 h and 1.28% (v/v) inoculum size] and [8% (w/v) feathers, 98.45 h, 3.9% (v/v) inoculum size] to achieve the highest levels of soluble proteins (1.25-1.7 mg/ml) and NH(2)-free amino groups (245.82-270.0 μmol leucine/ml), respectively upon using three optimized feathers-based media. These values represented 83.67-100% and 100% adequacy for the models of soluble proteins and NH(2)-free amino groups, respectively. While, [8.23% (w/v) sheep wool, 5.52% (v/v) inoculum size and 46.58 h] and [8.33% (w/v) sheep wool, 5.89% (v/v) inoculum size and 63.46 h] were the optimal sets of predicted and validated levels of the above variables to achieve the highest yields of soluble proteins (3.4-4.6 mg/ml) and NH(2)-free amino groups (290.9-302.0 μmol leucine/ml), respectively upon using three optimized sheep wool-based media. These values represented 100% adequacy for the models of soluble proteins and NH(2)-free amino groups. By the end of the optimization strategy, a fold enhancement (2.14-2.43 and 1.78-2.12) in the levels of released soluble proteins and NH(2)-free amino groups, respectively was obtained upon using three optimized feathers-based media. However, a fold enhancement (4.25-5.75 and 2.42-2.5) in the levels of soluble proteins and NH(2)-free amino groups, respectively was obtained upon using three optimized sheep wool-based media. Data would encourage pilot scale optimization of the biodegradation of these wastes.

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“…Likewise, similar results were obtained with the Vibrio sp. strain Kr2 but at pH values ranging from 6 to 8 and temperature of 30°C (Grazziotin et al, 2007). Other alkaline proteases from B. pumilus with high keratinolytic activity were also reported to accomplish alone the dehairing process on bovine hair (Kumar et al, 2008), cowhides (Wang et al, 2007a) and goatskins (Huang et al, 2003).…”

Section: Dehairing Utility Of Sapbmentioning

confidence: 99%