Production of Fructose and Ethanol from Cane Molasses Using Saccharomyces cerevisiae ATCC 36858

Abstract

SummaryThe purpose of this research was to study the possibility of the production of ethanol and enriched fructose syrups from sugar cane molasses using the yeast Saccharomyces cerevisiae ATCC 36858. In batch experiments with a total sugar concentration of between 96.7 g/l and 323.5 g/l, the fructose yield was above 90% of the theoretical value. The ethanol yield and volumetric productivity were in the range of 66% and 77% of the theoretical value, and between 0.53 g ethanol/l × h and 3.15 g ethanol/l × h, respectively. The fructose fraction in the carbohydrates content of the produced syrups was more than 95% when the total initial sugar concentration in the medium was below 273.8 g/l. Some oligosaccharides and glycerol were also produced in all tested media. The maximum amount of produced oligosaccharides including raffinose accounted for 13.4 g/l in the cane molasses medium with 323.5 g/l sugars in the initial phase of the fermentation process. The oligosaccharides produced and raffinose were completely consumed by the end of the fermentation process when the total initial sugar concentration was less than 191.3 g/l. The glycerol concentration was below 9.9 g/l. These findings are useful in the production of ethanol and high fructose syrups using sugar cane molasses.

Improvement of ethanol yield from raw corn flour by Rhizopus sp.

“…5). As the viscosity of the raw corn flour was low, and the content of reducing sugar, which can inhibit ethanol production with high concentration (Atiyeh and Duvnjak 2003) was maintained at subinhibitory levels, therefore, 60 g of raw corn flour were added entirely to the medium at the beginning of the fermentation. The conversion efficiency of corn flour to ethanol was 94.5% of the theoretical ethanol yield.…”

1,3-Propanediol production in a two-step process fermentation from renewable feedstock

“…This concentration is within the range showed to exert inhibition (Heux et al 2006;Remize et al 1999Remize et al , 2001. In a previous work with S. cerevisiae, the increase in molasses concentration resulted in a significant decrease of growth rate, biomass yield, and ethanol productivity, although with a higher range of concentrations tested (from 96.7 till 323.5 g l −1 ) (Atiyeh and Duvnjak 2003). For the second issue, we found that direct inoculation of an engineered strain of C. acetobutylicum DG1 (pSPD5) into the culture broth successfully converted glycerol previously produced by yeast into 1,3-PD.…”

Sugar Beet Cultivation in India: Prospects for Bio-Ethanol Production and Value-Added Co-Products

“…Commercially available yeasts help in the conversion of glucose (produced after enzymatic hydrolysis through invertase enzyme) to ethanol under an environment where oxygen is deficit. Atiyeh and Duvnjak ( 2003 ) revealed that Saccharomyces cerevisiae ATCC 36859 is one of the microbes which have efficiency towards higher production of ethanol from beet molasses. Besides, certain bacteria like Spirochaeta, etc., are also helpful in converting glucose to ethanol by the Embden Meyerhof pathway under an anaerobic environment.…”