Production of gluten-free wheat starch by peptidase treatment

Impact of the preparation procedure on gliadin, glutenin and gluten contents of wheat starches determined by RP-HPLC and ELISA

“…These values were in the same order of magnitude as those reported by Walter et al [26], who used the same washing procedure. Commercial industrially purified WSt typically had gluten contents ranging from 5 to 360 mg/kg (ELISA) [9].…”

“…The treatment of WSt with a prolyl endopeptidase from Aspergillus niger (AN-PEP) is a promising option to completely degrade gluten and remove its CD-toxicity [26], and this could be incorporated into the industrial purification process.…”

“…Two 19 g portions (a and b) of each wheat dough (mixing times 2, 12 and 22 min) were washed once with 530 ml distilled water for 10 min by a Glutomatic 2200 gluten washer (Perten, Hägersten, Sweden) [26]. In a similar experiment, two 19 g portions of each wheat dough were washed with 530 ml salt solution (0.4 mol/l NaCl) by the gluten washer.…”

Prolyl-specific peptidases for applications in food protein hydrolysis

“…The hydrolysis products may be detected by means of MALDI-TOF-MS (Stepniak et al 2006), ESI-MS (Mika et al 2015) or HPLC-UV (Shan et al 2004(Shan et al , 2005. Additionally, immunological tests, such as Western blots or enzyme-linked immunosorbent assays (ELISA), have been used (Stepniak et al 2006, Walter et al 2014. Immunological tests are especially important in cases where PsP are used for hydrolysis of gluten which can cause celiac disease (cf.…”

“…As a result, immune-sensitive gluten peptides did not reach the duodenum compartment, where they trigger inflammatory reactions. Walter et al (2014) tested AN-PEP also for the degradation of celiac disease relevant peptides and compared the activity to PsP containing bran extracts from germinated cereals. Different from the extracts of germinated bran, AN-PEP was able to reduce the gluten content of three types of wheat starch below the critical level of 20 mg kg 1 which was determined by means of competitive ELISA using the R5 antibody (RIDASCREEN® Gliadin competitive, R-Biopharm, Darmstadt, Germany).…”