Production of high-content galacto-oligosaccharide by enzyme catalysis and fermentation with Kluyveromyces marxianus

Cheng, Chao-Chun; Yu, Mei‐Ching; Cheng, Tzu-Chien; Sheu, Dey-Chyi; Duan, Kow‐Jen; Tai, Wei-Lun

doi:10.1007/s10529-006-9169-5

Cited by 7 publications

(4 citation statements)

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“…Among the two strains, K. marxianus DMB Km‐RK had better performance, resulting in a yield of 97.5% and a final purity of 100%. These observations are in good agreement with the yields reported in previous studies on K. lactis (Li et al, 2008; Santibáñez et al, 2017; Sun et al, 2016) and K. marxianus (Cheng et al, 2006; Guerrero et al, 2014). However, initial cell loads (0.0005–0.0007 g cell /g carbohydrate ) used in this study are considerably lower than in the previous reports by Santibáñez et al (2017), Sun et al (2016) and Li et al (2008) (0.375, 0.08 and 2.5 g cell /g carbohydrate , respectively).…”

Section: Resultssupporting

confidence: 92%

“…Cheng et al (2006) showed that K. marxianus ATCC 56497 was able to consume both lactose and monosaccharides when its fermentation was combined with enzymatic synthesis of low‐purity GOS. A disadvantage of K. marxianus is, however, the close to complete removal of disaccharides that differ from lactose structurally (i.e., possess glycosidic bonds other than the ß(1‐6) linkage of lactose).…”

Section: Introductionmentioning

confidence: 99%

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Production of high‐purity galacto‐oligosaccharides by depleting glucose and lactose from galacto‐oligosaccharide syrup with yeasts

Pázmándi

Kovács

Balga

et al. 2020

Yeast

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Galacto-oligosaccharides (GOS) are prebiotic compounds, widely used as ingredients in various food, nutraceutical and pharmaceutical products. Enzymatic synthesis of GOS results in low-purity products that contain high amounts of glucose and lactose beside the valuable GOS. In this study, a systematic approach was used to develop yeast-based fermentation strategies to purify crude GOS. Potentially applicable yeast strains were identified based on an extensive search in literature databases followed by a series of laboratory-scale fermentation tests. Single-and two-step fermentation processes were designed for the removal of glucose alone or together with lactose from crude GOS syrup. Single-step fermentation trials with two strains of previously unreported species, Cyberlindnera jadinii NCAIM Y.00499 and Kluyveromyces nonfermentans NCAIM Y.01443, resulted in purified products free of both glucose and ethanol from a crude GOS syrup diluted to 15 and 10 w/v%, respectively. Simultaneous removal of glucose and lactose was achieved by Kluyveromyces marxianus DMB Km-RK in a single-step fermentation process with a yield of 97.5% and final purity of 100%. A two-step fermentation approach was designed to allow conversion of a glucose-free product into a high-purity GOS by removing glucose with C. jadinii Y.00499 in the first step, and lactose by Kluyveromyces lactis DMB Kl-RK in the second step, resulting in a final product with a yield of 100% and a final purity of 92.1%. These results indicate that the selected nonconventional yeasts are promising candidates for the removal of non-GOS components from commercial crude GOS products by selective fermentation.

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Section: Resultssupporting

confidence: 92%

Section: Introductionmentioning

confidence: 99%

Production of high‐purity galacto‐oligosaccharides by depleting glucose and lactose from galacto‐oligosaccharide syrup with yeasts

Pázmándi

Kovács

Balga

et al. 2020

Yeast

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“…Similar to the synthesis of FOS, the initial concentration of lactose determines the chemical composition of the synthesized GOS, the more concentrated the substrate, the larger the synthesized GOS (129, 142). Some authors (150) claimed that the initial lactose concentration is directly related with the enzyme activity explaining that higher concentrations favor an increase in GOS yield (121, 127, 138).…”

Section: Gosmentioning

confidence: 99%

“…Selective fermentation is another strategy to remove monosaccharides (135, 142, 194, 195). It basically consists on an anaerobic glycolysis by yeasts, in which the monosaccharides are converted into ethanol and CO 2 (72).…”

Section: Gosmentioning

confidence: 99%

Technological Aspects of the Production of Fructo and Galacto-Oligosaccharides. Enzymatic Synthesis and Hydrolysis

et al. 2019

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Fructo- and galacto-oligosaccharides (FOS and GOS) are non-digestible oligosaccharides with prebiotic properties that can be incorporated into a wide number of products. This review details the general outlines for the production of FOS and GOS, both by enzymatic synthesis using disaccharides or other substrates, and by hydrolysis of polysaccharides. Special emphasis is laid on technological aspects, raw materials, properties, and applications.

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Biodegradation of lindane using a novel yeast strain, Rhodotorula sp. VITJzN03 isolated from agricultural soil

Salam

Lakshmi

Das

et al. 2012

World J Microbiol Biotechnol

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Lindane is a notorious organochlorine pesticide due to its high toxicity, persistence in the environment and its tendency to bioaccumulate. A yeast strain isolated from sorghum cultivation field was able to use lindane as carbon and energy source under aerobic conditions. With molecular techniques, it was identified and named as Rhodotorula strain VITJzN03. The effects of nutritional and environmental factors on yeast growth and the biodegradation of lindane was investigated. The maximum production of yeast biomass along with 100 % lindane mineralization was noted at an initial lindane concentration of 600 mg l(-1) within a period of 10 days. Lindane concentration above 600 mg l(-1) inhibited the growth of yeast in liquid medium. A positive relationship was noted between the release of chloride ions and the increase of yeast biomass as well as degradation of lindane. The calculated degradation rate and half life of lindane were found to be 0.416 day(-1) and 1.66 days, respectively. The analysis of the metabolites using GC-MS identified the formation of seven intermediates including γ-pentachlorocyclohexane(γ-PCCH), 1,3,4,6-tetrachloro-1,4-cyclohexadiene(1,4-TCCHdiene), 1,2,4-trichlorobenzene (1,2,4 TCB), 1,4-dichlorobenzene (1,4 DCB), chloro-cis-1,2-dihydroxycyclohexadiene (CDCHdiene), 3-chlorocatechol (3-CC) and maleylacetate (MA) derivatives indicating that lindane degradation follows successive dechlorination and oxido-reduction. Based on the results of the present study, the possible pathway for lindane degradation by Rhodotorula sp. VITJzN03 has been proposed. To the best of our knowledge, this is the first report on lindane degradation by yeast which can serve as a potential agent for in situ bioremediation of medium to high level lindane-contaminated sites.

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Production of high-content galacto-oligosaccharide by enzyme catalysis and fermentation with Kluyveromyces marxianus

Cited by 7 publications

References 0 publications

Production of high‐purity galacto‐oligosaccharides by depleting glucose and lactose from galacto‐oligosaccharide syrup with yeasts

Production of high‐purity galacto‐oligosaccharides by depleting glucose and lactose from galacto‐oligosaccharide syrup with yeasts

Technological Aspects of the Production of Fructo and Galacto-Oligosaccharides. Enzymatic Synthesis and Hydrolysis

Biodegradation of lindane using a novel yeast strain, Rhodotorula sp. VITJzN03 isolated from agricultural soil

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