Production of high degree polymerized chitooligosaccharides in a membrane reactor using purified chitosanase from Bacillus cereus

Lin, Yu‐Wei; Hsiao, Yi-Chien; Chiang, Been‐Huang

doi:10.1016/j.foodres.2009.06.008

Cited by 31 publications

(16 citation statements)

References 25 publications

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“…On the other hand, nonspecific enzymes are inexpensive and commercially available and have been used in the industry for years to produce COS with relatively low cost. Lin et al [105] reported the hydrolytic susceptibility of chitosan to a wide range of enzymes, including glycanases, proteases and lipases derived from bacterial, fungal, mammalian and plant sources. Enzymatic chitosan hydrolysis can be performed either with soluble or immobilized enzymes column or membrane reactors.…”

Section: Chitooligosaccharidesmentioning

confidence: 99%

A Future Perspective in Crop Protection: Chitosan and its Oligosaccharides

Hemantaranjan

Katiyar

Singh

et al. 2014

APAR

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Studies on the biological activities of chitosan and its oligomers have been increasing, as no single type of chitosan or its oligomers exerts all of the above bioactivities. Moreover, different chitosan derivatives and enzymatic products have different structures and physicochemical properties, which may result in novel bioactivities or novel findings in known bioactive compounds. Chitosan oligosaccharides also have been shown to induce various plant defense-related cellular responses and possess by themselves antimicrobial properties against a wide spectrum of phytopathogens [18,19]. The biological activity of COS is known to depend on their structure. Chitosan derivatives have a wide scope of application and regulate the immune system of plants and induce the excretion of resistant enzymes. Moreover, chitosan not only activates the cells, but also improves its disease and insect resistant ability. There is a great concern about the consequences of utilization of chemicals on health and environment, so other means of controlling diseases have become a vehement necessity. There are other well-known problems in agriculture sector, such as: disease resistance to bactericides, fungicides and high costs to develop new products, so the scientists are interested in biological control agents and naturally occurring fungicides. Keeping in view the important role of chitosan and COS in agriculture, this review is aimed at investigating the efficacy of chitosan and its derivative on crop protection. ChitosanChitosan is derived from chitin, a polysaccharide found in exoskeleton of shellfish such as shrimp, lobster or crabs and cell wall of fungi [20]. Chitosan poly (1,4)-2-amino-2-deoxy-β-D IntroductionNow-a-day's new approaches and practices are being developed for sustainable agriculture for various crops and vegetables. Therefore, various work is going on chitosan association for different plants growth in various laboratories of India. Chitosan and its oligosaccharides are natural molecule that are applied to crops with the aim of reducing or replacing more costly and environmentally damaging chemical bactericides. With reduced input costs and the potential for increased yields, farmers could gain substantial benefits from these applications of chitosan and its oligosaccharides to crops.Chitosan is a one of the most abundant natural amino polysaccharides extracted from the exoskeleton of crustaceans and insect, from fungal cell walls, etc. These substances have a wide variety of applications in agricultural and biotechnological industries [1,2]. Among the novel families of biological macromolecules, whose relevance is becoming increasingly evident, are chitin and its main derivative, chitosan. Both are the simplest linear polysaccharide composed of α, 1-4 linked D-glucosamine (GlcN) and N-acetyl-D-glucosamine (GlcNAc) with various compositions of these two monomers. Chitin and its derivatives have become a promising alternative treatment due to its natural character, antifungal activity and elicitation of defense resp...

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Section: Chitooligosaccharidesmentioning

confidence: 99%

A Future Perspective in Crop Protection: Chitosan and its Oligosaccharides

Hemantaranjan

Katiyar

Singh

et al. 2014

APAR

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“…Amino columns [18,19,20,21,22,23,24] and size-exclusion columns [25,26] are typically coupled to this detector, which has low sensitivity and is not suitable for gradient elution. With these drawbacks, researchers are compelled to develop another methodology.…”

Section: Introductionmentioning

confidence: 99%

Validated HPAEC-PAD Method for the Determination of Fully Deacetylated Chitooligosaccharides

Cao

Wu²,

et al. 2016

IJMS

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An efficient and sensitive analytical method based on high-performance anion exchange chromatography with pulsed amperometric detection (HPAEC-PAD) was established for the simultaneous separation and determination of glucosamine (GlcN)1 and chitooligosaccharides (COS) ranging from (GlcN)2 to (GlcN)6 without prior derivatization. Detection limits were 0.003 to 0.016 mg/L (corresponding to 0.4–0.6 pmol), and the linear range was 0.2 to 10 mg/L. The optimized analysis was carried out on a CarboPac-PA100 analytical column (4 × 250 mm) using isocratic elution with 0.2 M aqueous sodium hydroxide-water mixture (10:90, v/v) as the mobile phase at a 0.4 mL/min flow rate. Regression equations revealed a good linear relationship (R2 = 0.9979–0.9995, n = 7) within the test ranges. Quality parameters, including precision and accuracy, were fully validated and found to be satisfactory. The fully validated HPAEC-PAD method was readily applied for the quantification of (GlcN)1–6 in a commercial COS technical concentrate. The established method was also used to monitor the acid hydrolysis of a COS technical concentrate to ensure optimization of reaction conditions and minimization of (GlcN)1 degradation.

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“…For example, chitotriose was obtained as the major product by hydrolyzing chitosan with chitosanase from Gongronella sp. JG , while heptamers and oligomers with higher polymerization degrees were obtained by chitosanase CBCIII from Bacillus cereus (Lin et al, 2009). So application of novel and potential chitosanases has recently gained importance as an advanced alternative approach for producing chitooligosaccharides (Wee et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

Statistical optimization of medium compositions for chitosanase production by a newly isolated Streptomyces albus

Cheng

Wang

Hong

2012

Braz. J. Chem. Eng.

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-The nutritional medium compositions required for chitosanase production by Streptomyces albus YT2 were optimized, and the strain was isolated from the coastal soil of Bohai Sea. Plackett-Burman design was applied to evaluate the effect of different medium compositions obtained by one-factor-at-a-time experiments. Glucose, peptone and MgSO 4 were found to influence the activity of chitosanase significantly. The steepest ascent method was employed to approach the experimental design space, followed by the use of Box-Behnken design and response surface methodology for further optimization of the chitosanase production. The medium composition for optimal production of chitosanase was as follows: 0.5 g/L chitosan, 55 g/L glucose, 1 g/L peptone, 2 g/L (NH 4 ) 2 SO 4 , 8 g/L urea, 1 g/L NH 4 Cl, 3 g/L KH 2 PO 4 , 1 g/L FeSO 4 ·7H 2 O, 2 g/L ZnSO 4 ·7H 2 O, 2 g/L CaCl 2 ·6H 2 O, 2 g/L MnSO 4 ·H 2 O and 2 g/L NaCl. The chitosanase production was found to increase from 11.56 U/mL to 39.87 U/mL, a 3.47-fold increase compared with that using the original medium.

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Production of high degree polymerized chitooligosaccharides in a membrane reactor using purified chitosanase from Bacillus cereus

Cited by 31 publications

References 25 publications

A Future Perspective in Crop Protection: Chitosan and its Oligosaccharides

A Future Perspective in Crop Protection: Chitosan and its Oligosaccharides

Validated HPAEC-PAD Method for the Determination of Fully Deacetylated Chitooligosaccharides

Statistical optimization of medium compositions for chitosanase production by a newly isolated Streptomyces albus

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