Production of high-Quality Ornithogalum thyrsoides cut flowers in one year from in vitro propagated plantlets influenced by plant growth regulators

Joung, Young Hee; Wu, Xiaosheng; Roh, Mark S.

doi:10.1016/j.scienta.2020.109395

Cited by 3 publications

(2 citation statements)

References 16 publications

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“…Therefore, optimization of the somatic embryogenesis protocol is the rst and most important step in gene transformation studies, but it is a laborious, time-consuming, and complex process (Hesami and Jones 2020, . In in vitro cultures, somatic embryogenesis processes such as dedifferentiation, re-differentiation, or differentiation are caused by genetic and environmental factors (Gürel et al 2019, Joung et al 2020). Therefore, it will be di cult to predict various cases, including the rate of callus formation, and the rate of embryogenesis, as well as the optimization of the factors involved in them through conventional mathematical methods (Hesami and Jones 2020, Salehi et al 2020).…”

Section: Introductionmentioning

confidence: 99%

Prediction of the concentration of plant growth regulators for somatic embryogenesis and regeneration of Hyoscyamus niger using Box–Behnken design of response surface methodology

Ahmadpour

Zanjani

Garoosi

et al. 2023

Plant Cell Tiss Organ Cult

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Optimizing of the in vitro culture condition, somatic embryogenesis, and plant regeneration is the rst step in transformation. In this study, the response surface methodology was used to optimize and predict the concentrations of TDZ, NAA, BAP, and kinetin for callus formation, and regeneration by indirect somatic embryogenesis of Hyoscyamus niger. The results proved that TDZ is more e cient than NAA for the regeneration of H. niger. By using different concentrations of TDZ, BAP, and kinetin, the highest percentage of callusing (100.00%), callus fresh weight (1207.75 mg/explant), percentage of somatic embryogenesis (92.66%) and shooting (93.30%), and the number of the shoot (7.75 per callus) was recorded from petiole explants on MS medium containing 0.25 mg/L TDZ and 1 mg/L BAP, leaf explants by using 0.5 mg/L TDZ and 1 mg/L kinetin, petiole explants with addition 0.5 mg/L TDZ, leaf explants by using 0.50 mg/L TDZ and 1 mg/L kinetin, and petiole explants with addition 0.25 mg/L TDZ and 1 mg/L BAP, respectively. Also, the highest percentage of callusing (100.00%), callus fresh weight (3273.64 mg/explant), percentage of somatic embryogenesis (100%), shooting (100.00%), and the number of the shoot (8.61 per callus) were predicted by culturing of petiole explant on the MS medium containing 0.

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Section: Introductionmentioning

confidence: 99%

Prediction of the concentration of plant growth regulators for somatic embryogenesis and regeneration of Hyoscyamus niger using Box–Behnken design of response surface methodology

Ahmadpour

Zanjani

Garoosi

et al. 2023

Plant Cell Tiss Organ Cult

View full text Add to dashboard Cite

show abstract

“…In vitro culture consists of highly complex and nonlinear processes such as dedifferentiation, re-differentiation, or differentiation due to the genetic and environmental factors [18][19][20][21]. Therefore, it would be difficult to predict different in vitro culture parameters such as callogenesis rate, embryogenesis rate, and the number of somatic embryos as well as optimize factors involved in these parameters by simple conventional mathematical methods [22][23][24].…”

mentioning

confidence: 99%

Development of support vector machine-based model and comparative analysis with artificial neural network for modeling the plant tissue culture procedures: effect of plant growth regulators on somatic embryogenesis of chrysanthemum, as a case study

et al. 2020

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Background: Optimizing the somatic embryogenesis protocol can be considered as the first and foremost step in successful gene transformation studies. However, it is usually difficult to achieve an optimized embryogenesis protocol due to the cost and time-consuming as well as the complexity of this process. Therefore, it is necessary to use a novel computational approach, such as machine learning algorithms for this aim. In the present study, two machine learning algorithms, including Multilayer Perceptron (MLP) as an artificial neural network (ANN) and support vector regression (SVR), were employed to model somatic embryogenesis of chrysanthemum, as a case study, and compare their prediction accuracy. Results: The results showed that SVR (R 2 > 0.92) had better performance accuracy than MLP (R 2 > 0.82). Moreover, the Non-dominated Sorting Genetic Algorithm-II (NSGA-II) was also applied for the optimization of the somatic embryogenesis and the results showed that the highest embryogenesis rate (99.09%) and the maximum number of somatic embryos per explant (56.24) can be obtained from a medium containing 9.10 μM 2,4-dichlorophenoxyacetic acid (2,4-D), 4.70 μM kinetin (KIN), and 18.73 μM sodium nitroprusside (SNP). According to our results, SVR-NSGA-II was able to optimize the chrysanthemum's somatic embryogenesis accurately. Conclusions: SVR-NSGA-II can be employed as a reliable and applicable computational methodology in future plant tissue culture studies.

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Prediction of the concentration of plant growth regulators for somatic embryogenesis and regeneration of Hyoscyamus niger using Box-Behnken design of response surface methodology

Ahmadpour

Zanjani

Garoosi

et al. 2023

Preprint

View full text Add to dashboard Cite

Optimizing of the in vitro culture condition, somatic embryogenesis, and plant regeneration is the first step in transformation. In this study, the response surface methodology was used to optimize and predict the concentrations of TDZ, NAA, BAP, and kinetin for callus formation, and regeneration by indirect somatic embryogenesis of Hyoscyamus niger. The results proved that TDZ is more efficient than NAA for the regeneration of H. niger. By using different concentrations of TDZ, BAP, and kinetin, the highest percentage of callusing (100.00%), callus fresh weight (1207.75 mg/explant), percentage of somatic embryogenesis (92.66%) and shooting (93.30%), and the number of the shoot (7.75 per callus) was recorded from petiole explants on MS medium containing 0.25 mg/L TDZ and 1 mg/L BAP, leaf explants by using 0.5 mg/L TDZ and 1 mg/L kinetin, petiole explants with addition 0.5 mg/L TDZ, leaf explants by using 0.50 mg/L TDZ and 1 mg/L kinetin, and petiole explants with addition 0.25 mg/L TDZ and 1 mg/L BAP, respectively. Also, the highest percentage of callusing (100.00%), callus fresh weight (3273.64 mg/explant), percentage of somatic embryogenesis (100%), shooting (100.00%), and the number of the shoot (8.61 per callus) were predicted by culturing of petiole explant on the MS medium containing 0.11 mg/L TDZ and 1.253 mg/L BAP, leaf explant by using 0.52 mg/L TDZ, petiole explant by using 0.533 mg/L TDZ and 1 mg/L BAP, leaf explant with addition 0.18 mg/L TDZ and 2.89 mg/L BAP and petiole explant by using 0.1 mg/L TDZ and 3 mg/L BAP.

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Production of high-Quality Ornithogalum thyrsoides cut flowers in one year from in vitro propagated plantlets influenced by plant growth regulators

Cited by 3 publications

References 16 publications

Prediction of the concentration of plant growth regulators for somatic embryogenesis and regeneration of Hyoscyamus niger using Box–Behnken design of response surface methodology

Prediction of the concentration of plant growth regulators for somatic embryogenesis and regeneration of Hyoscyamus niger using Box–Behnken design of response surface methodology

Development of support vector machine-based model and comparative analysis with artificial neural network for modeling the plant tissue culture procedures: effect of plant growth regulators on somatic embryogenesis of chrysanthemum, as a case study

Prediction of the concentration of plant growth regulators for somatic embryogenesis and regeneration of Hyoscyamus niger using Box-Behnken design of response surface methodology

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