Production of human CD59-transgenic pigs by embryonic germ cell nuclear transfer

Ahn, Kwang Sung; Jung, Won Seok; Park, Jin-Ki; Sorrell, Alice M.; Heo, Soon Young; Kang, Jee Hyun; Woo, Jonghye; Choi, Bong-Hwan; Chang, Won-Kyong; Shim, Hosup

doi:10.1016/j.bbrc.2010.08.125

Cited by 9 publications

(6 citation statements)

References 21 publications

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“…In fact, we observed more severe developmental defects in the cloned piglets derived from EGCs than when using comparable transgenic Yucatan fibroblasts (see (Schmidt et al, 2015) for a thorough description on developmental defects in piglets made by SCNT). Ahn and coworkers (Ahn et al, 2010) also succeed in creating CD59-transgenic piglets using porcine EGC for SCNT. They transferred 1980 reconstructed embryos to 10 recipient sows resulting in 5 pregnancies and 15 piglets of which 5 died within the first 24 hours (Ahn et al, 2010) and thus also indicating that the EGCs do not have the same clonogenicity as murine embryonic cells (Oback, 2009).…”

Section: Discussionmentioning

confidence: 99%

“…Ahn and coworkers (Ahn et al, 2010) also succeed in creating CD59-transgenic piglets using porcine EGC for SCNT. They transferred 1980 reconstructed embryos to 10 recipient sows resulting in 5 pregnancies and 15 piglets of which 5 died within the first 24 hours (Ahn et al, 2010) and thus also indicating that the EGCs do not have the same clonogenicity as murine embryonic cells (Oback, 2009). Finally, creating and maintaining porcine EGCs are laborious processes that require special technical skills (Petkov et al, 2011) It should be mentioned that the data for this paper is not collected as part of an experimental set up made to explore the correlation between cell differentiation potency and clonogenicity.…”

Section: Discussionmentioning

confidence: 99%

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Evaluation of porcine stem cell competence for somatic cell nuclear transfer and production of cloned animals

Secher

Liu

Petkov³

et al. 2017

Animal Reproduction Science

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Porcine somatic cell nuclear transfer (SCNT) has been used extensively to create genetically modified pigs, but the efficiency of the methodology is still low. It has been hypothesized that pluripotent or multipotent stem cells might result in increased SCNT efficacy as these cells are closer than somatic cells to the epigenetic state found in the blastomeres and therefore need less reprogramming. Our group has worked with porcine SCNT during the last 20 years and here we describe our experience with SCNT of 3 different stem cell lines. The porcine stem cells used were: Induced pluripotent stem cells (iPSCs) created by lentiviral doxycycline-dependent reprogramming and cultered with a GSK3β- and MEK-inhibitor (2i) and leukemia inhibitor factor (LIF) (2i LIF DOX-iPSCs), iPSCs created by a plasmid-based reprogramming and cultured with 2i and fibroblast growth factor (FGF) (2i FGF Pl-iPSCs) and embryonic germ cells (EGCs), which have earlier been characterized as being multipotent. The SCNT efficiencies of these stem cell lines were compared with that of the two fibroblast cell lines from which the iPSC lines were derived. The blastocyst rates for the 2i LIF DOX-iPSCs were 14.7%, for the 2i FGF Pl-iPSC 10.1%, and for the EGCs 34.5% compared with the fibroblast lines yielding 36.7% and 25.2%. The fibroblast- and EGC-derived embryos were used for embryo transfer and produced live offspring at similar low rates of efficiency (3.2 and 4.0%, respectively) and with several instances of malformations. In conclusion, potentially pluripotent porcine stem cells resulted in lower rates of embryonic development upon SCNT than multipotent stem cells and differentiated somatic cells.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Evaluation of porcine stem cell competence for somatic cell nuclear transfer and production of cloned animals

Secher

Liu

Petkov³

et al. 2017

Animal Reproduction Science

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“…These include pronuclear injection [4], oocyte transduction [34,35], embryo transduction [36,37] fibroblast transduction followed by SCNT [35], sperm-mediated gene transfer (SMGT) [38], fibroblast transfection followed by SCNT [34,35], and embryonic germ cell transfection followed by NT [39]. The main advantage of pronuclear injection is that large constructs can be integrated.…”

Section: Methodsmentioning

confidence: 99%

Completion of the swine genome will simplify the production of swine as a large animal biomedical model

Wolf²,

et al. 2012

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BackgroundAnatomic and physiological similarities to the human make swine an excellent large animal model for human health and disease.MethodsCloning from a modified somatic cell, which can be determined in cells prior to making the animal, is the only method available for the production of targeted modifications in swine.ResultsSince some strains of swine are similar in size to humans, technologies that have been developed for swine can be readily adapted to humans and vice versa. Here the importance of swine as a biomedical model, current technologies to produce genetically enhanced swine, current biomedical models, and how the completion of the swine genome will promote swine as a biomedical model are discussed.ConclusionsThe completion of the swine genome will enhance the continued use and development of swine as models of human health, syndromes and conditions.

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“…To overcome the problem of HAR, GM pigs expressing the human membrane-bound complementary proteins have been generated (Table 1) such as GM pigs expressing the human decay-accelerating factor, DAF (CD55, MIM125240) (Murakami et al 2000;Lavitrano et al 2002;Lee et al 2011), the human complement regulatory protein CD59 (MIM107271) (Fodor et al 1994;Ahn et al 2010), and the human membrane cofactor protein CD46 (MIM120920) (Diamond et al 2001). Protection against HAR and extended survival have been observed upon transplantation of hearts, kidneys, or vascularized organs from these GM pigs into nonhuman primates (McCurry et al 1995;Zaidi et al 1998;Chen et al 1999;Diamond et al 2001).…”

Section: Gm Pigs For Xenotransplantationmentioning

confidence: 99%

Genetically modified pigs for biomedical research

Luo

Lin

Bolund

et al. 2012

J of Inher Metab Disea

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During the last two decades, pigs have been used to develop some of the most important large animal models for biomedical research. Advances in pig genome research, genetic modification (GM) of primary pig cells and pig cloning by nuclear transfer, have facilitated the generation of GM pigs for xenotransplantation and various human diseases. This review summarizes the key technologies used for generating GM pigs, including pronuclear microinjection, sperm‐mediated gene transfer, somatic cell nuclear transfer by traditional cloning, and somatic cell nuclear transfer by handmade cloning. Broadly used genetic engineering tools for porcine cells are also discussed. We also summarize the GM pig models that have been generated for xenotransplantation and human disease processes, including neurodegenerative diseases, cardiovascular diseases, eye diseases, bone diseases, cancers and epidermal skin diseases, diabetes mellitus, cystic fibrosis, and inherited metabolic diseases. Thus, this review provides an overview of the progress in GM pig research over the last two decades and perspectives for future development.

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Production of human CD59-transgenic pigs by embryonic germ cell nuclear transfer

Cited by 9 publications

References 21 publications

Evaluation of porcine stem cell competence for somatic cell nuclear transfer and production of cloned animals

Evaluation of porcine stem cell competence for somatic cell nuclear transfer and production of cloned animals

Completion of the swine genome will simplify the production of swine as a large animal biomedical model

Genetically modified pigs for biomedical research

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