Production of<i>Bacillus amyloliquefaciens</i>OG and its metabolites in renewable media: valorisation for biodiesel production and<i>p</i>-xylene decontamination

Etchegaray, Augusto; Coutte, François; Châtaigné, Gabrielle; Béchet, Max; Santos, Ramon Henrick Zorzetto dos; Leclère, Valérie; Jacques, Philippe

doi:10.1139/cjm-2016-0288

Cited by 17 publications

(14 citation statements)

References 46 publications

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“…Lactate production reached 2.83 g/L after 24 h of culture. In addition, acetoin production reached its maximum level after 24 h of growth (7.5 g/L) and then showed a slight decrease and completely converted after 48 h. Similar patterns were observed for the production of these metabolites by Bacillus amyloliquefaciens OG [ 34 ]. The primary metabolite production has been demonstrated previously with other strains of Bacillus using glucose as the main source of carbon and limited oxygen [ 35 , 36 ].…”

Section: Resultssupporting

confidence: 62%

Enhancement of Surfactin and Fengycin Production byBacillus mojavensisA21: Application for Diesel Biodegradation

Hmidet

Ayed

Jacques

2017

BioMed Research International

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This work concerns the study of the enhancement of surfactin and fengycin production by B. mojavensis A21 and application of the produced product in diesel biodegradation. The influences of the culture medium and cells immobilization were studied. The highest lipopeptides production was achieved after 72 hours of incubation in a culture medium containing 30 g/L glucose as carbon source and a combination of yeast extract (1 g/L) and glutamic acid (5 g/L) as nitrogen sources with initial pH 7.0 at 30°C and 90% volumetric aeration. The study of primary metabolites production showed mainly the production of acetoin, with a maximum production after 24 h of strain growth. The use of immobilized cells seemed to be a promising method for improving lipopeptides productivity. In fact, the synthesis of both lipopeptides, mainly fengycin, was greatly enhanced by the immobilization of A21 cells. An increase of diesel degradation capacity of approximately 20, 27, and 40% in the presence of 0.5, 1, and 2 g/L of produced lipopeptides, respectively, was observed. Considering these properties, B. mojavensis A21 strain producing a lipopeptide mixture, containing both surfactin and fengycin, may be considered as a potential candidate for future use in bioremediation and crop protection.

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Section: Resultssupporting

confidence: 62%

Enhancement of Surfactin and Fengycin Production byBacillus mojavensisA21: Application for Diesel Biodegradation

Hmidet

Ayed

Jacques

2017

BioMed Research International

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“…Substitution of glucose with glycerol in LA +G medium resulted not only in maintaining more stable pH levels, but also in slightly better growth and in an almost threefold increase in PF yield (Table 1 , P < 0.005). The beneficial effect of glycerol on surfactin production by B. amyloliquefaciens (velezensis) was recently demonstrated [ 24 ]. This suggests a positive role of glycerol, amino acids, and complex additives (yeast extract and/or peptone) in promoting growth and PF production by strain BD5.…”

Section: Resultsmentioning

confidence: 99%

“…For example, B. subtilis strains are capable of producing up to 12 surfactin analogs and P. fluorescens SS101 produces eight or more massetolide A isoforms [ 1 , 5 ]. Even different LP families can be produced by one strain, as shown for different B. subtilis and B. amyloliquefaciens ( velezensis ) strains that produce several iturin, fengycin, and surfactin analogs [ 13 , 24 ]. Proportions of LP analogs differ among bacterial strains and it can change in response to certain conditions, e.g.…”

Section: Introductionmentioning

confidence: 99%

High-throughput optimization of medium components and culture conditions for the efficient production of a lipopeptide pseudofactin by Pseudomonas fluorescens BD5

et al. 2018

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BackgroundLipopeptides are a promising group of surface-active compounds of microbial origin (biosurfactants). These diverse molecules are produced mainly by Bacillus and Pseudomonas strains. Because of their attractive physiochemical and biological properties, biosurfactants are considered to be “green and versatile molecules of the future”. The main obstacles in widespread use of biosurfactants are mainly their low yields and high production costs. Pseudofactin (PF) is a lipopeptide produced by Pseudomonas fluorescens BD5. Recently, we identified two analogues, PF1 (C16-Val) and PF2 (C16-Leu), and reported that PF2 has good emulsification and foaming activities, as well as antibacterial, antifungal, anticancer, and antiadhesive properties. Reported production of PF in a mineral salt medium was approximately 10 mg/L.ResultsHere, we report successful high-throughput optimization of culture medium and conditions for efficient PF production using P. fluorescens BD5. Compared with production in minimal medium, PF yield increased almost 120-fold, up to 1187 ± 13.0 mg/L. Using Plackett–Burman and central composite design methodologies we identified critical factors that are important for efficient PF production, mainly high glycerol concentration, supplementation with amino acids (leucine or valine) and complex additives (e.g. tryptone), as well as high culture aeration. We also detected the shift in a ratio of produced PF analogues in response to supplementation with different amino acids. Leucine strongly induces PF2 production, while valine addition supports PF1 production. We also reported the identification of two new PF analogues: PF3 (C18-Val) and PF4 (C18-Leu).ConclusionsIdentification of critical culture parameters that are important for lipopeptide production and their high yields can result in reduction of the production costs of these molecules. This may lead to the industrial-scale production of biosurfactants and their widespread use. Moreover, we produced new lipopeptide pure analogues that can be used to investigate the relationship between the structure and biological activity of lipopeptides.Electronic supplementary materialThe online version of this article (10.1186/s12934-018-0968-x) contains supplementary material, which is available to authorized users.

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“…Liu, Yang, Yang, Ye, & Mu, reported that the addition of different amino acids to the cultures had a significant influence on the proportion of surfactin variants with different fatty acids. The peptide moiety contains two l ‐Leu and two d ‐Leu residues (Figure ), and the content of Leu in cells is crucial for the synthesis of surfactin (Etchegaray et al, ). Moreover, the research of Coutte et al indicated that leucine overproduction significantly improved the production of surfactin in recombinant Bacillus subtilis 168 derivatives (Coutte et al, ).…”

Section: Introductionmentioning

confidence: 99%

Genome and transcriptome analysis of Bacillus velezensis BS‐37, an efficient surfactin producer from glycerol, in response to d‐/l‐leucine

Zhou

Lin

et al. 2019

MicrobiologyOpen

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Surfactin is one of the most widely studied biosurfactants due to its many potential applications in different fields. In the present study, Bacillus velezensis BS‐37, initially identified as a strain of Bacillus subtilis, was used to efficiently produce surfactin with the addition of glycerol, an inexpensive by‐product of biodiesel production. After 36 hr of growth in glycerol medium, the total surfactin concentration reached more than 1,000 mg/L, which was two times higher than that in sucrose medium. Moreover, the addition of l‐ and d‐Leu to the culture medium had opposite effects on surfactin production by BS‐37. While surfactin production increased significantly to nearly 2,000 mg/L with the addition of 10 mM l‐Leu, it was dramatically reduced to about 250 mg/L with the addition of 10 mM d‐Leu. To systemically elucidate the mechanisms influencing the efficiency of this biosynthesis process, we sequenced the genome of BS‐37 and analyzed changes of the transcriptome in glycerol medium in response to d‐/l‐leucine. The RPKM analysis of the transcriptome of BS‐37 showed that the transcription levels of genes encoding modular surfactin synthase, the glycerol utilization pathway, and branched‐chain amino acid (BCAA) synthesis pathways were all at a relatively high level, which may offered an explanation why this strain can efficiently use glycerol to produce surfactin with a high yield. Neither l‐Leu nor d‐Leu had a significant effect on the expression of genes in these pathways, indicating that l‐Leu plays an important role as a precursor or substrate involved in surfactin production, while d‐Leu appears to act as a competitive inhibitor. The results of the present study provide new insights into the synthesis of surfactin and ways of its regulation, and enrich the genomic and transcriptomic resources available for the construction of high‐producing strains.

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Production ofBacillus amyloliquefaciensOG and its metabolites in renewable media: valorisation for biodiesel production andp-xylene decontamination

Cited by 17 publications

References 46 publications

Enhancement of Surfactin and Fengycin Production byBacillus mojavensisA21: Application for Diesel Biodegradation

Enhancement of Surfactin and Fengycin Production byBacillus mojavensisA21: Application for Diesel Biodegradation

High-throughput optimization of medium components and culture conditions for the efficient production of a lipopeptide pseudofactin by Pseudomonas fluorescens BD5

Genome and transcriptome analysis of Bacillus velezensis BS‐37, an efficient surfactin producer from glycerol, in response to d‐/l‐leucine

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